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I-Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Umsila Wegundane)(ye-Genotyping)
Sebenzisa ngokuqondile i-lysate yomsila wegundane njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
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I-Animal Tissue Direct PCR kit-UNG (ngaphandle Kwamathuluzi Okusampula) Iphrothokholi
Sebenzisa ngokuqondile izicubu zezilwane i-lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.Kwengezwe uhlelo lwe-UNG lokulwa nokunukubezeka ukuze kuqedwe imibono engamanga.
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
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I-Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent(Umsila Wegundane)(ye-Genotyping)
Sebenzisa ngokuqondile i-lysate yomsila wegundane njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
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I-Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Umsila Wegundane)(ye-Genotyping)
I-lysate yomsila wegundane isetshenziswa ngokuqondile njengesifanekiso sokukhulisa i-PCR ngaphandle kokukhipha okuhlukile kwe-DNA, esheshayo futhi ezwela kakhulu.Kwengezwe uhlelo lwe-UNG lokulwa nokunukubezeka ukuze kuqedwe imibono engamanga.
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
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I-Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent(i-zebrafish)
Sebenzisa ngokuqondile i-zebrafish lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
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Ikhithi yesitshalo eqondile ye-PCR Plus (ngaphandle kwamathuluzi okusampula) I-Protocol Direct PCR evela ku-Plant Material
Sebenzisa ngokuqondile i-polysaccharide ne-polyphenol leaf lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.
◮Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)
◮Impahla encane
◮Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
◮2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela
◮Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50
◮Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96
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I-Plant Seed Direct PCR Kit I/II (ngaphandle kwamathuluzi okusampula) I-Protocol Plant Direct PCR Kits
◮Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)
◮Impahla encane
◮Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
◮2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela
◮Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50
◮Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96
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Iqabunga lesitshalo I-PCR eqondile kanye nekhit-UNG (ngaphandle kwamathuluzi okusampula) Iphrothokholi eqondile ye-PCR evela ku-Plant Material
Sebenzisa ngokuqondile i-polysaccharide ne-polyphenol leaf lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.
◮Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)
◮Impahla encane
◮Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
◮2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela
◮Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50
◮Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96
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I-RT-qPCR Easyᵀᴹ (Isinyathelo Esisodwa)-Taqman
- One-Ikhithi yesinyathelo inika amandla ukulotshwa okuhlanekezelwe kanye ne-PCR ukuthi kwenziwe ngeshubhu efanayo, kudingeka kuphela ukwengeza isifanekiso se-RNA, iziqalo ezithile ze-PCR kanye ne-RNase-Free ddH2O.
- Ukuhlaziywa kobuningi besikhathi sangempela kwe-RNA yegciwane noma ukulandelela i-RNA kungenziwa ngokushesha nangokunembile.
- Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
- Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela okuphezulu kokutholwa, ukuqina okushisayo okuqinile, nokubekezelelana okungcono.
- RT-qPCR EasyTM(One Isinyathelo) -Ikhithi ye-Taqman iza nodayi wereferensi wangaphakathi we-ROX, ongasetshenziswa ukuqeda ingemuva lesignali namaphutha esignali phakathi kwemithombo, elungele abasebenzisi bokugcina ukuyisebenzisa kumamodeli ahlukene wamathuluzi e-PCR amaningi.
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I-RNAlater (Yokuqiniswa kwe-RNA) Isixazululo Sokuqinisa I-RNAlater
Ukulungiswa okusheshayo kwezicubu zesampula yezilwane ezisanda kuthathwa, kuvimbela umsebenzi we-RNase, kuvikela i-RNA ekonakalisweni.
-Ama-reagents asetshenziswa ngokuqondile, isinyathelo esisodwa sisendaweni, futhi i-RNA iqiniswa ngokushesha futhi ivikelwe.
-Ukusebenza ekamelweni lokushisa, kulula, kuphephile futhi akunabuthi.
-Ukuqeda inkathazo yeqhwa elomile noma isiqandisi esinezinga lokushisa eliphansi lokulondoloza izicubu.
-Izicubu zingagcinwa isikhathi eside ngaphandle kwengozi yokuwohloka kwe-RNA, kuqinisekiswe idatha ethembekile yephrofayili yokubonakaliswa kofuzo.
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Iqhawe le-PCR
◮Ukwethembeka okuphezulu: 6izikhathi ezejwayelekile ze-Taq enzyme;
◮Ngokushesha aisivinini sokukhuliswa:isivinini sokukhulisa i-5-10sec/kb, okuyizikhathi ezingu-6-12 kune-enzyme ye-Taq evamile;
◮Ukuvumelana nezimo kwesifanekiso: ingakhulisa ngempumelelo izifanekiso ze-DNA eziyinkimbinkimbi ezinokuqukethwe okuphezulu kwe-GC futhi okunzima ukukukhulisa;
◮PhezuluUkusebenza kahle kokukhulisa: inani lemijikelezo yokukhulisa i-amplification liphansi kunalelo le-enzyme ye-Taq evamile;
◮Skakhudlwana Ukubekezelelana kwemvelo: ibekwe ku-37°C isonto lonke, igcina ngaphezu kwalokho90%umsebenzi;
◮Inomsebenzi we-DNA polymerase ongu-5'→3' kanye nomsebenzi we-exonuclease ongu-5'→3', ngaphandle komsebenzi we-exonuclease ongu-3'→5'.
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I-PCR Easy
Isistimu yokusabela eyingqayizivele kanye nokusebenza kahle okuphezulu kwe-Taq DNA Polymerase kwenza ukusabela kwe-PCR kube nokusebenza kahle kokukhulisa ukuphakama, ukucacisa nokuzwela.
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