I-China Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(for Genotyping) Umkhiqizi Nomhlinzeki |I-Foregene

I-Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Umsila Wegundane)(ye-Genotyping)

I-Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(for Genotyping) Isithombe Esifakiwe

Incazelo yekhithi:

I-lysate yomsila wegundane isetshenziswa ngokuqondile njengesifanekiso sokukhulisa i-PCR ngaphandle kokukhipha okuhlukile kwe-DNA, esheshayo futhi ezwela kakhulu.Kwengezwe uhlelo lwe-UNG lokulwa nokunukubezeka ukuze kuqedwe imibono engamanga.

◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.

◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Izincazelo

Le kit isebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze ikhulule ngokushesha i-DNA ye-genomic emisileni yegundane, ezindlebeni, emisipha kanye namanye amasampula ezicubu zokusabela kwe-PCR, ngakho ifaneleka ngokukhethekile ukuhlolwa kofuzo okukhulu.

Inqubo yokukhipha i-DNA ye-genomic ku-lysis buffer iqedwa phakathi kwemizuzu eyi-10-30 ku-65°C.Azikho ezinye izinqubo ezidingekayo njengokususwa kwamaprotheni kanye ne-RNA, futhi i-trace DNA ekhishiwe ingasetshenziswa njengesifanekiso sokusabela kwe-PCR.

2× M1-PCR Easy^TMI-Mix (UNG) isebenzisa i-dUTP esikhundleni se-dTTP ngesisekelo se-2× M1-PCR Easy^TMMix (UNG), bese wengeza i-UNG enzyme (Uracil-N-glycosylase) engehlisa isithunzi isifanekiso esiqukethe i-dUTP ngesikhathi esifanayo.Ngaphambi kokusabela kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukuthunaza umkhiqizo we-PCR oqukethe i-uracil.I-enzyme ye-UNG ngeke ibe namuphi umthelela kusifanekiso esingaqukethe i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa nokuvimbela amathuba okuhlolwa kofuzo okukhulu.Ukungcoliswa kwemikhiqizo ye-PCR kwenzekile.

I-D-Taq DNA polymerase iyi-polymerase ye-DNA ethuthukiswe ngokukhethekile yi-Foregene ukuze kutholakale ukusabela okuqondile kwe-PCR.I-D-Taq DNA polymerase inokubekezelela okunamandla ezinhlobonhlobo ze-PCR reaction inhibitors, futhi ingakhulisa kahle amanani omkhondo we-DNA ezinhlelweni ezihlukene zokusabela eziyinkimbinkimbi, futhi isivinini sokukhulisa singafinyelela ku-2Kb/min, okulungele ikakhulukazi ukusabela kwe-Direct PCR.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I	Buffer MP
	I-Foregene Protease Plus
	I-6× I-DNA Yokulayisha Ibhafa
Ingxenye II	2× M1 PCR Kulula^TMImiksi(UNG)
Iziyalezo

Izici&izinzuzo

■Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

■ Isidingo sesampula sincane, njengoba nje kungahlolwa izicubu zegundane eziyi-5mg noma umsila wegundane ongu-2-5mm.

■ Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

■ Uhlelo lwe-PCR lokulwa nokunukubezeka 2×I-M1-PCR Kulula^TMI-Mix (UNG), eqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi iqinisekise ukucaciswa nokunemba kokukhulisa.

Imingcele yekhithi

Ububanzi bokusebenza: umsila wegundane, indlebe yegundane, imisipha nezinye izicubu.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Ukugeleza komsebenzi

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer AL ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

I-Reagent Foregene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-Reagent 2×M1 PCR EasyTM Mix(UNG), uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe ngaphakathi kwezinsuku eziyi-10).

Okwedlule: I-Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Umsila Wegundane)(ye-Genotyping)

Olandelayo: I-Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent(Umsila Wegundane)(ye-Genotyping)

Bhala umyalezo wakho lapha futhi usithumelele wona