Iseli Ingqikithi ye-RNA Isolate Kit

Iseli Ingqikithi ye-RNA Isolate Kit

Incazelo Yekhithi:

Le khithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle i-RNA esezingeni eliphezulu nekhwalithi ephezulu kumaseli akhuliswe kuma-96, 24, 12, kanye nama-6-well plate. Ikhithi inikeza i-DNA-Cleaning Column ephumelelayo, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA. Ukusebenza kulula futhi konga isikhathi; Ikholomu ye-RNA kuphela ingabopha i-RNA kahle ngefomula elihlukile. Inani elikhulu lamasampuli lingacutshungulwa ngasikhathi sinye.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ehlanziwe ingehliswa; iBuffer RW1, iBuffer RW2 buffer washing system ibeka i-RNA etholakalayo ngaphandle kwamaprotheni, i-DNA, i-ion, kanye nokungcola kwe-organic compound.


Imininingwane yomkhiqizo

Amathegi womkhiqizo

Imininingwane

Ama-preps angama-50, ama-preps angama-200

Izingxenye Kit

I-Buffer cRL1, I-Buffer cRL2

Ibhafa RW1, Ibhafa RW2

I-RNase-Free ddH2O, Ikholomu Yokuhlanza i-DNA

Ikholomu RNA-Kuphela

Izici nezinzuzo

The Yonke le nqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza ngeqhwa kanye nokushisa okuncane.
■ Ikhithi yonke ayi-RNase-Free, asikho isidingo sokukhathazeka ngokonakala kwe-RNA.
■ Ikholomu Yokuhlanza iDNA ibopha i-DNA ngokukhethekile, ukuze ikhithi isuse ukungcoliswa kwe-genomic DNA ngaphandle kokungeza i-DNase eyengeziwe.
■ Ukukhiqizwa okuphezulu kwe-RNA: Ikholomu ye-RNA kuphela nefomula eyingqayizivele ingahlanza kahle i-RNA.
■ Isivinini esisheshayo: kulula ukusebenza futhi kungaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Akukho reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe ihlanzekile kakhulu, ayinayo amaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlolwa okuhlukahlukene okulandelayo.

Kit isicelo

Kukulungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulile kuma-96, 24, 12, nama-6-well plates.

Ukuhamba komsebenzi

cell total RNA

Umdwebo

Cell Total RNA Isolation Kit Work Flow1

Umdwebo webhethri we-agarose we-Cell Total RNA Isolate Kit uphathe izinombolo ezihlukene ezingenhla zamaseli, i-20μl yevolumu yokuxhunyaniswa, yathatha i-R2 1% ehlanjululwe ngokuphelele.

Isitoreji nempilo yeshalofu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemuva kokungeza i-2-hydroxy-1-ethanethiol (ngokuzithandela).


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