Iseli Direct RT qPCR Kit

Iseli Direct RT qPCR Kit

Incazelo Yekhithi:

Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer olungakhipha ngokushesha i-RNA kusuka kumasampuli amaseli akhulisiwe okuphendula kwe-RT-qPCR, ngaleyo ndlela kuqedwe inqubo yokuhlanzwa ye-RNA echitha isikhathi futhi ekhandlayo. Isifanekiso se-RNA singatholakala ngemizuzu engu-7 nje kuphela. Ama-reagents we-5 × Direct RT kanye ne-2 × Direct qPCR Mix-SYBR anikezwe yikhithi angathola ngokushesha nangempumelelo imiphumela yangempela ye-PCR yokulinganisa.

I-5 × Direct RT Mix ne-2 × Direct qPCR Mix-SYBR inokubekezelelana okuqinile kwe-inhibitor, futhi i-lysate yamasampuli ingasetshenziswa njengethempulethi ye-RT-qPCR ngqo. Le khithi iqukethe i-RNA ephezulu enobudlelwano obuphezulu be-Foregene reverse transcriptase, ne-Hot D-Taq DNA polymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.


Imininingwane yomkhiqizo

Amathegi womkhiqizo

Imininingwane

200×20μl Ama-Rxns, 1000×20μl Ama-Rxns

Izingxenye Kit

Ingxenye I

Ibhafa CL

I-Foregene Protease Plus II

IBuffer ST

Ingxenye II

Isisuli seDNA

I-5 × Direct RT Mix

2 × I-qPCR Mix-SYBR eqondile

Udayi wereferensi ongu-50 × ROX

I-dNH2O yamahhala ye-RNase

Imiyalo

Izici nezinzuzo

■  Ilula futhi isebenza ngempumelelo: ngobuchwepheshe be-Cell Direct RT, amasampula e-RNA angatholakala ngemizuzu engu-7 nje.

The Ukufunwa kwesampula kuncane, njengoba amaseli ayi-10 angahlolwa.

■ Ukwenza okuphezulu: ingathola ngokushesha i-RNA kumaseli akhiwe ngamapuleti angama-384, 96, 24, 12, 6-well.

■ I-DNA Eraser ingasusa ngokushesha izakhi zofuzo ezikhishiwe, inciphise kakhulu umthelela emiphumeleni yokuhlola elandelayo.

■ Uhlelo olwenzelwe i-RT ne-qPCR lwenza ukuthi ukuloba okubhalwe phansi kwe-RT-PCR okuyizinyathelo ezimbili kusetshenziswe kakhudlwana futhi i-PCR icace kakhudlwana, futhi imelane kakhulu nama-RT-qPCR reaction inhibitors.

Kit isicelo

Ububanzi besicelo: amaseli akhulile.

- I-RNA ekhishwe yisampula lysis: isebenza kuphela kuthempulethi ye-RT-qPCR yale khithi.

- Ikhithi ingasetshenziselwa izinhloso ezilandelayo: ukuhlaziywa kwezakhi zofuzo, ukuqinisekiswa komphumela wokuthulisa izakhi zofuzo ze-siRNA, ukuhlolwa kwezidakamizwa, njll.

Umdwebo

Cell Direct RT qPCR diagram

Isitoreji nempilo yeShelf

Ingxenye I yale kit kufanele igcinwe ku-4 ℃; Ingxenye II kufanele igcinwe ku- -20 ℃.

 I-Foregene Protease Plus II kufanele igcinwe ku-4Yebo, ungafrizi ngo -20 ℃.

 Reagent 2×I-Direct qPCR Mix-SYBR kufanele igcinwe ku -20ebumnyameni; uma isetshenziswa njalo, ingagcinwa naku-4℃ ukugcina okwesikhashana (sebenzisa kuze kuphele izinsuku eziyi-10).


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