Khipha ngokushesha nangempumelelo izingcezu ezincane ze-RNA ze-20-200nt miRNA, siRNA, snRNA ezicutshini zezilwane ezihlukahlukene namaseli.
◆Yonke le nqubo iqhutshwa ekamelweni lokushisa (15-25°C), ngaphandle kokugeza kweqhwa kanye ne-centrifugation enezinga lokushisa eliphansi.
◆Isethi egcwele yekhithi i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
◆Ikholomu Yokuhlanza I-DNA ibophezela ngokuqondile i-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
◆Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
◆Isivinini esisheshayo: Ukusebenza kulula futhi kungaqedwa phakathi nemizuzu engama-30.
◆Ukuphepha: akukho kukhishwa kwe-organic reagent edingekayo.
◆Ikhwalithi ephezulu: Izingcezu ze-RNA ezikhishiwe zinokuhlanzeka okuphezulu, azinawo amaprotheni nokunye ukungcola, futhi zingahlangabezana nezidingo zomfula ongezansi.
◆Izinhlelo zokusebenza zokuhlola ezihlukahlukene