Ikhithi yesitshalo eqondile ye-PCR Plus (ngaphandle kwamathuluzi okusampula) I-Protocol Direct PCR evela ku-Plant Material
Izincazelo
Lo mkhiqizo usebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze uhlukanise amaqabunga ezitshalo.I-lysate ingasetshenziswa njengesifanekiso ngaphandle kokuhlanza.Ngemva kokwengeza ama-primer, i-PCR Mix yale khithi ingasetshenziselwa ukukhulisa.
Imininingwane
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Izingxenye zekhithi
Ingxenye I | Buffer PS1 |
Isilondolozi se-P2 | |
6×I-DNA Loading Buffer | |
Ingxenye II | Buffer PS2 |
2× Leaf PCR EasyTMHlanganisa |
Izici&izinzuzo
■ Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo
■ Impahla encane
■ Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
■ 2 × Mix, ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela uhlelo lokusabela
■ Ukulungiselela isifanekiso esisheshayo kungaqedwa ngemizuzu eyi-10, ukusabela kwe-PCR kungaqedwa okungenani ngemizuzu engama-50
■ Ukusabela okuphezulu kwe-throughput-lysis kungaqedwa ngepuleti le-PCR le-96-well
Imingcele yekhithi
Isicelo: Ukuhlonza izitshalo ze-transgenic, i-genotyping, njll.
Isampula: amaqabunga esitshalo anothe nge-polysaccharide noma i-polyphenol
Usayizi wesampula: Ububanzi 2-3mm (indlela eqondile), ububanzi 5-7mm (indlela ye-lysis)
Ibanga lokutholwa: Umkhiqizo we-PCR ≤1kb