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Ikhithi yesitshalo eqondile ye-PCR Plus (ngaphandle kwamathuluzi okusampula) I-Protocol Direct PCR evela ku-Plant Material

Incazelo yekhithi:

Sebenzisa ngokuqondile i-polysaccharide ne-polyphenol leaf lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.

Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)

Impahla encane

Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya

2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela

Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50

Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96

amandla angaphambili

 

 

 


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Izincazelo

Lo mkhiqizo usebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze uhlukanise amaqabunga ezitshalo.I-lysate ingasetshenziswa njengesifanekiso ngaphandle kokuhlanza.Ngemva kokwengeza ama-primer, i-PCR Mix yale khithi ingasetshenziselwa ukukhulisa.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I Buffer PS1
Isilondolozi se-P2
6×I-DNA Loading Buffer
Ingxenye II Buffer PS2
2× Leaf PCR EasyTMHlanganisa

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo
■ Impahla encane
■ Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
■ 2 × Mix, ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela uhlelo lokusabela
■ Ukulungiselela isifanekiso esisheshayo kungaqedwa ngemizuzu eyi-10, ukusabela kwe-PCR kungaqedwa okungenani ngemizuzu engama-50
■ Ukusabela okuphezulu kwe-throughput-lysis kungaqedwa ngepuleti le-PCR le-96-well

Imingcele yekhithi

Isicelo: Ukuhlonza izitshalo ze-transgenic, i-genotyping, njll.

Isampula: amaqabunga esitshalo anothe nge-polysaccharide noma i-polyphenol

Usayizi wesampula: Ububanzi 2-3mm (indlela eqondile), ububanzi 5-7mm (indlela ye-lysis)

Ibanga lokutholwa: Umkhiqizo we-PCR ≤1kb

Ukugeleza komsebenzi

plant leaf direct PCR plus-workflow

Electropherogram

plant leaf ngqo PCR plus

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