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I-Plant Seed Direct PCR Kit I/II (ngaphandle kwamathuluzi okusampula) I-Protocol Plant Direct PCR Kits

I-Plant Seed Direct PCR Kit I/II (ngaphandle kwamathuluzi okusampula) Iphrothokholi Yesitshalo Esiqondile Se-PCR Kits Isithombe Esifakiwe
  • I-Plant Seed Direct PCR Kit I/II (ngaphandle kwamathuluzi okusampula) I-Protocol Plant Direct PCR Kits
  • I-Plant Seed Direct PCR Kit I/II (ngaphandle kwamathuluzi okusampula) I-Protocol Plant Direct PCR Kits
  • I-Plant Seed Direct PCR Kit I/II (ngaphandle kwamathuluzi okusampula) I-Protocol Plant Direct PCR Kits
  • I-Plant Seed Direct PCR Kit I/II (ngaphandle kwamathuluzi okusampula) I-Protocol Plant Direct PCR Kits

Incazelo yekhithi:

◮Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)

◮Impahla encane

◮Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya

◮2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela

◮Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50

◮Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96

amandla angaphambili


Landa njenge-PDF

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Izincazelo

Lo mkhiqizo usebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze ukhiphe imbewu yezitshalo.I-lysate ingasetshenziswa njengesifanekiso ngaphandle kokuhlanza.Ngemva kokwengeza ama-primer, i-PCR Mix yale khithi ingasetshenziselwa ukukhulisa.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Plant Seed Direct PCR Kit I

Ingxenye I

Isilondolozi SP1

Isilondolozi se-SP2

I-6×I-DNA Yokulayisha Ibhafa

Ingxenye II

2×Imbewu PCR KululaTMHlanganisa

Plant Seed Direct PCR Kit II

Ingxenye I

Isilondolozi SP1

Isilondolozi se-SP2

I-6×I-DNA Yokulayisha Ibhafa

Ingxenye II

2×Imbewu PCR KululaTMHlanganisa

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo

■ Impahla encane

■ Simple-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya

■ 2X Mix-ukunciphisa amaphutha okulayisha isampula kanye nesikhathi sokulungiselela uhlelo lokusabela

■ Ngokushesha-ukulungiswa kwesifanekiso kungaqedwa ngemizuzu eyi-10, ukusabela kwe-PCR kungaqedwa ngemizuzu engama-50

■ Ukusebenza okuphezulu-ukusabela kwe-lysis kungaqedwa ngepuleti le-PCR elingu-96.

Imingcele yekhithi

Isicelo: Ukuhlonza izitshalo ze-transgenic, i-genotyping, njll.

Isampula: Tshala imbewu noma izicubu ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Usayizi wesampula: Imbewu eyodwa yonke, izicubu zezitshalo eziyi-1-100mg

Ibanga lokutholwa: Umkhiqizo we-PCR ≤1kb

Ukugeleza komsebenzi

tshala imbewu ngqo PCR

Electropherogram

tshala imbewu ngqo i-PCR-DIAGRAM
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