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  • I-RT-qPCR Kit II eqondile

    I-RT-qPCR Kit II eqondile

    ◮Ikhithi eqondile ye-RT-qPCR, isebenzisa i-Foreasy Reverse Transcriptase ne-Foreasy HS Taq DNA Polymerase eyakhiwe yi-Foregene, ene-react buffer eyingqayizivele, yenza le khithi ibe nokumelana okuqinile nokuhambisana, futhi ingahlola ngokuqondile ukusetshenziswa kwesistimu ye-Foregene Lysis njengesifanekiso.Le khithi ingasebenza ekutholeni ikhithi ye-COVID-19 nucleic acid kanye namanye amabhaktheriya e-pathogenic nucleic acid yokuthuthukiswa kwekhithi.

     

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  • I-RT-qPCR Kit III eqondile

    I-RT-qPCR Kit III eqondile

    ◮Ikhithi eqondile ye-RT-qPCR, isebenzisa i-Foreasy Reverse Transcriptase ne-Foreasy HS Taq DNA Polymerase eyakhiwe yi-Foregene, ene-react buffer eyingqayizivele, yenza le khithi ibe nokumelana okuqinile nokuhambisana, futhi ingahlola ngokuqondile ukusetshenziswa kwesistimu ye-Foregene Lysis njengesifanekiso.Le khithi ingasebenza ekutholeni ikhithi ye-COVID-19 nucleic acid kanye namanye amabhaktheriya e-pathogenic nucleic acid yokuthuthukiswa kwekhithi.

     

     

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  • Ikhithi ye-RT-qPCR eqondile

    Ikhithi ye-RT-qPCR eqondile

    ◮ Ikhithi eqondile ye-RT-qPCR, isebenzisa i-Foreasy Reverse Transcriptase ne-Foreasy HS Taq DNA Polymerase eyakhiwe yi-Foregene, ene-react buffer eyingqayizivele, yenza le khithi ibe nokumelana okuqinile nokuhambisana, futhi ingahlola ngokuqondile ukusetshenziswa kwesistimu ye-Foregene Lysis njengesifanekiso.Le khithi ingasebenza ekutholeni ikhithi ye-COVID-19 nucleic acid kanye namanye amabhaktheriya e-pathogenic nucleic acid yokuthuthukiswa kwekhithi.

     

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  • Umenzeli Wokukhishwa Kwesampula

    Umenzeli Wokukhishwa Kwesampula

    ◮Kulula ukusebenza:isampula lysis ngenkathi isampula iqoqo

    ◮Kushesha futhi kulula:Asikho isidingo sokukhishwa kwe-nucleic acid, okufanele ukutholwa ngezinga elikhulu

    ◮Ububanzi bohlelo lokusebenza:I-PCR ngokuqondile ngemva kokuqoqwa kwesampula

    ◮Iphephile futhi inokwethenjelwa:Yenza igciwane lingasebenzi ngokushesha, qinisekisa ukuphepha ngezinga elikhulu kakhulu

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  • Ikhithi ye-ForeDirect RT-qPCR

    Ikhithi ye-ForeDirect RT-qPCR

    ◮Ukuze uthole i-RNA eqondile yokukhulisa isikhathi sangempela kusuka kumaqoqo we-swab ngaphandle kwezinqubo zokuhlanza i-RNA.Le khithi iqeda imijikelezo ye-qRT-PCR phakathi nehora elilodwa.Ingxube ye-enzyme iyinhlanganisela elungiselelwe ye-Reverse Transcriptase, i-Hot-Start Taq DNA polymerase, i-RNase inhibitor.I-Reaction Buffer iqukethe zonke izingxenye ezidingekayo, okuhlanganisa izingxenye zebhafa ezithuthukisiwe, uMg2+, i-dUTP, nama-dNTP.

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  • I-Foreasy M-MLV Reverse Transcriptase

    I-Foreasy M-MLV Reverse Transcriptase

    I-Foreasy M-MLV Reverse Transcriptase iyireverse transcriptase evezwa kubhaktheriya enziwe nge-E. coli kusetshenziswa ubuchwepheshe bokuhlanganisa kabusha izakhi zofuzo.Kuyi-recombinant DNA polymerase ehlanganisa umucu we-DNA ohambisanayo kusukela ku-RNA enomucu owodwa, i-DNA, noma i-RNA:ingxube ye-DNA.Ayinawo umsebenzi we-RNase H, ukuzinza okuqinile, ukuhlobana okuqinile kwe-RNA, nokuzwela okuphezulu kokutholwa.

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  • I-Foreasy RNase Inhibitor

    I-Foreasy RNase Inhibitor

    ◮I-RNase inhibitor entsha etholakala kumagundane evezwa kubhaktheriya eyenziwe yi-E. coli isebenzisa ubuchwepheshe bokuhlanganiswa kabusha kwezakhi zofuzo.I-inhibitor ivimbela umsebenzi we-RNase ngokungahlanganisi ne-RNase 1:1, ngaleyo ndlela ivikele.I-RNAubuqotho , futhi ingavimbela ngempumeleloiRNase A, B, C  umsebenzi , kodwa hhayi i-RNase T1, T2, H, njll.; Iyabuyiseleka emuva ekubopheni kwe I-RNase Inhibitor ne-RNase, futhi inkimbinkimbi ingahlukaniswa ngama-reagents e-urea kanye ne-sulfhydryl, ukuze ukuguqulwa kabusha kwe-RNase kanye ne-inhibitor kungasebenzi ngendlela engenakuhlehliswa.

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  • I-Foreasy HS Taq DNA Polymerase

    I-Foreasy HS Taq DNA Polymerase

    ◮Ukucaciswa okuphezulu: I-enzyme enomsebenzi ophezulu wokuqalisa ukushisa.

    ◮Ukukhulisa Ngokushesha: 10 sec/kb.

    ◮Ukuvumelana nezimo kwesifanekiso esiphezulu : kungasetshenziswa ukukhulisa kahle OkuphezuluGCinanifuthiizifanekiso ezihlukahlukene ze-DNA okunzima ukuzikhulisa.

    ◮Ukwethembeka okuqinile: Ukwethembeka izikhathi ezingu-6of i-Taq enzyme ejwayelekile.

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  • I-Foreasy Taq DNA Polymerase

    I-Foreasy Taq DNA Polymerase

    ◮Ukucaciswa okuphezulu: I-enzyme inomsebenzi othile wokuqalisa ukushisa.

    ◮Ukukhulisa Ngokushesha: 10 sec/kb.

    ◮Isifanekiso esizivumelanisa nezimo kakhulu: singasetshenziswa ukuze kukhuliswe kahle inani eliphakeme le-GC, izifanekiso ezihlukahlukene okunzima ukuzikhulisa ze-DNA.

    ◮Ukuthembeka okuqinile: I-Taq Enzyme evamile izikhathi ezi-6.

    ◮Ukuzinza okuqinile kokushisa: Ingabekwa ku-37 °C isonto lonke futhi igcine umsebenzi ongaphezu kuka-90%.

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