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Ikhithi yokuhlukanisa i-RNA yezilwane

Incazelo yekhithi:

Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.Lonke uhlelo alunayo i-RNase-Free

Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column

Khipha i-DNA ngaphandle kokwengeza i-DNase

Kulula—yonke imisebenzi iqedwa ezingeni lokushisa legumbi

Ukusheshisa—ukusebenza kungaqedwa ngemizuzu engu-30

Iphephile—ayikho i-organic reagent esetshenzisiwe

Ukuhlanzeka okuphezulu—OD260/280≈1.8-2.1

foregene strength


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

FAQ

Kits Izincazelo

50 Preps, 200 Preps

Le kit isebenzisa i-spin ikholomu kanye nefomulaithuthukiswe inkampani yethu, ekwazi ukukhipha i-RNA ehlanzekile nesezingeni eliphezulu ezicutshini zezilwane ezihlukahlukene ngokusebenza kahle okuphezulu.Ihlinzeka ngekholomu ye-DNA-Cleaning ephumelelayo, engahlukanisa kalula futhi ikhangise i-DNA ye-genomic ku-supernatant kanye ne-tissue lysate, elula. kanye nokonga isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA futhi ingacutshungulwa kanyekanye ngefomula eyingqayizivele Amasampuli amaningi.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ekhishiwe ingonakalisiwe;I-Buffer RW1, i-Buffer RW2 isistimu yokuwasha ibhafa, ukuze i-RNA etholiwe ingabi nawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwenhlanganisela yezinto eziphilayo.

Izingxenye zekhithi

Ikhithi yokuhlukanisa i-RNA yezilwane

Izingxenye zekhithi RE-03011 RE-03014
50T 200T
I-Buffer RL1* 25ml 100ml
Isilondolozi RL2 15ml 60ml
Isilondolozi RW1* 25ml 100ml
Isilondolozi se-RW2 24ml 96ml
I-RNase-Free ddH2O 10ml 40ml
Ikholomu ye-RNA kuphela 50 200
Ikholomu Yokuhlanza I-DNA 50 200
Incwadi Yeziqondiso 1ucezu 1ucezu

Ulwazi lomkhiqizo

Ifomethi Spin ikholomu Ingxenye yokuhlanza I-Foregeneikholomu, reakahle
Flux 1-24amasampula Isikhathi sokulungiselela ~30imiz(amasampula angama-24)
Cukungena Ideskii-centrifuge Ukuhlukaniswa kwe-pyrolysis Ukuhlukaniswa kwe-Centrifugal
Isampula Izicubu zezilwane;iseli Inani lamasampuli Tudaba:10-20 mg; Iseli:(1-5)×106
Ivolumu ye-Elution 50-200μL Ivolumu yokulayisha ephezulu 850 ml

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

https://www.foreivd.com/reagent/rna-isolation-series/

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×106
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Ukugeleza komsebenzi

animal total RNA-simple workflow

Umdwebo