I-Animal Total RNA Isolate Kit

I-Animal Total RNA Isolate Kit

Incazelo Yekhithi:

Ikhithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle i-RNA ephelele nekhwalithi ephezulu kuzicubu zezilwane ezahlukahlukene. Inikeza ikholomu yokuhlanza i-DNA engasusa kalula i-genomic DNA kwi-supernatant ne-lysate yezicubu. Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA. Ikhithi ingacubungula inani elikhulu lamasampula ngasikhathi sinye.

Uhlelo lonke alunayo i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe. IBuffer RW1 neBuffer RW2 zingaqinisekisa ukuthi i-RNA etholakele ayingcoliswanga amaprotheni, iDNA, ions, kanye nezinhlanganisela zemvelo.


Imininingwane yomkhiqizo

Amathegi womkhiqizo

Imininingwane

Ama-preps angama-50, ama-preps angama-200

Izingxenye Kit

IBuffer RL1, iBuffer RL2

Ibhafa RW1, Ibhafa RW2

I-RNase-Free ddH2O, Ikholomu Yokuhlanza i-DNA

Ikholomu RNA-Kuphela

Izici nezinzuzo

■ Akunasidingo sokukhathazeka ngokonakala kwe-RNA; lonke uhlelo luyi-RNase-Free
■ Susa ngempumelelo i-DNA-using Column-Cleaning Column
■ Susa i-DNA ngaphandle kokungeza i-DNase
■ Ukusebenza okulula konke kuqedwa ekamelweni lokushisa
■ Ukusebenza ngokushesha kungaqedwa ngemizuzu engama-30
■ Ukuphepha-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260 / 280≈1.8-2.1

Kit isicelo

Kuyafaneleka ukukhishwa nokuhlanzwa kwe-RNA ephelele kusuka ezinhlobonhlobo zezicubu zezilwane ezintsha noma ezibandisiwe noma amaseli akhulisiwe.

Amapharamitha womkhiqizo

■ Izicelo ezisezansi: i-first-strand cDNA synthesis, i-RT-PCR, i-cloning cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Isikali: Izicubu 10-20mg, Amaseli (2-5) × 106
■ Ubukhulu besibopho se-DNA esibopha ikholomu yokuhlanzwa: 80 μg
■ Elution ivolumu: 50-200 μl

Ukuhamba komsebenzi

animal total RNA-simple workflow

Umdwebo

3 Animal Total RNA Isolation Kit7

I-Animal Total RNA Isolate Kit iphathwe ngama-20mg
Amasampula wegundane amasha, thatha i-RNA 1% ye-agar ehlanzekile engu-5%

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshalofu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside. I-Buffer RL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemuva kokungeza i-β- mercaptoethanol (ngokuzithandela).


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