Iqhawe le-PCR
Incazelo yekhithi:
◮Ukwethembeka okuphezulu: 6izikhathi ezejwayelekile ze-Taq enzyme;
◮Ngokushesha aisivinini sokukhuliswa:isivinini sokukhulisa i-5-10sec/kb, okuyizikhathi ezingu-6-12 kune-enzyme ye-Taq evamile;
◮Ukuvumelana nezimo kwesifanekiso: ingakhulisa ngempumelelo izifanekiso ze-DNA eziyinkimbinkimbi ezinokuqukethwe okuphezulu kwe-GC futhi okunzima ukukukhulisa;
◮PhezuluUkusebenza kahle kokukhulisa: inani lemijikelezo yokukhulisa i-amplification liphansi kunalelo le-enzyme ye-Taq evamile;
◮Skakhudlwana Ukubekezelelana kwemvelo: ibekwe ku-37°C isonto lonke, igcina ngaphezu kwalokho90%umsebenzi;
◮Inomsebenzi we-DNA polymerase ongu-5'→3' kanye nomsebenzi we-exonuclease ongu-5'→3', ngaphandle komsebenzi we-exonuclease ongu-3'→5'.
Izincazelo zekhithi
Lo mkhiqizo uhlanganisa isizukulwane esisha esiyingqayizivele sikaForegene se-Taq——Forregene Taq Hero, i-dNTP elungiselelwe, i-MgCl2, isilondolozi sokusabela, isithuthukisi sokusabela kwe-PCR, isikhuthazi kanye nesiqinisi.Iqhawe le-2 × PCRTMIsistimu ye-Mix inokubekezelela okuphezulu kuma-PCR inhibitors kunesistimu evamile ye-PCR Mix, futhi ingabhekana kalula nokukhulisa i-PCR yezifanekiso eziyinkimbinkimbi ezihlukahlukene.Isistimu yokusabela eyingqayizivele kanye nokusebenza kahle okuphezulu kwe-Taq Hero kwenza ukusabela kwe-PCR kube nokusebenza kahle kokukhulisa ukuphakama, ukucacisa nokuzwela.
Imininingwane
Ikhithi Series
PH-01013:2× PCR IqhaweTMHlanganisa 1.7ml × 3
PH-01014:2× PCR IqhaweTMHlanganisa 1.7ml × 6
PH-01015:2× PCR IqhaweTMHlanganisa 1.7ml × 24
Izingxenye zekhithi
2 × PCR IqhaweTMImiksi ihlanganisa:0.1U Taq Hero/μl, 400μM dNTPs, 20mM Tris-HCl(pH8.8),100mM KCl, 5mM MgCl2, ezinye iziqinisi nezithuthukisi.
Izici&izinzuzo
-Ukwethembeka okuphezulu:6izikhathi ezejwayelekile ze-Taq enzyme;
- Ngokushesha aisivinini sokukhuliswa:isivinini sokukhulisa i-5-10sec/kb, okuyizikhathi ezingu-6-12 kune-enzyme ye-Taq evamile;
-Ukuvumelana nezimo kwesifanekiso: ingakhulisa ngempumelelo izifanekiso ze-DNA eziyinkimbinkimbi ezinokuqukethwe okuphezulu kwe-GC futhi okunzima ukukukhulisa;
- EphakemeUkusebenza kahle kokukhulisa: inani lemijikelezo yokukhulisa i-amplification liphansi kunalelo le-enzyme ye-Taq evamile;
-Skakhudlwana Ukubekezelelana kwemvelo: ibekwe ku-37°C isonto lonke, igcina ngaphezu kwalokho90%umsebenzi;
-Inomsebenzi we-DNA polymerase ongu-5'→3' kanye nomsebenzi we-exonuclease ongu-5'→3', ngaphandle komsebenzi we-exonuclease ongu-3'→5'.
Isicelo sekhithi
- I-PCR yokukhulisa izingcezu ze-DNA
- Ukulebula kwe-DNA
- Ukulandelana kwe-DNA
- PCR plus A umsila
Isitoreji nempilo yeshelufu
Ingagcinwa isikhathi eside ku -20°C;ukuze isetshenziswe njalo, ingagcinwa ku-4°C isikhathi esifushane.
