Sithumele i-imeyili ukuze uthole ulwazi olwengeziwe:
E:overseas@foregene.com
I-Direct PCR iyindlela yokukhulisa i-DNA ngqo kusukaigazi, izicubu zezilwane,umsila wegundane,iseli,indlebe yegundane,idube izinhlanzi, amaqanda ezinhlanzi,tshala amaqabunga,imbewunoma elinye isampula lethishu yebhayoloji yoqobo ngaphandle kokwenza ukuhlukaniswa kwe-DNA nezinyathelo zokuyihlanza.Indlela ye-PCR eqondile inganciphisa kakhulu isikhathi sokuhlola, kanye nezindleko kumaphrojekthi we-genotyping kanye nenani eliphezulu.Iphinde inikeze inketho engcono uma ubhekene nezinselele zokukhulisa inani elincane kakhulu lesampula lapho isinyathelo sokuhlanza singase siholele ekulahlekeni kwesampula.
I-ForEGENE's PCR Mix inokumelana nokucindezeleka okuqinile, ngenxa yamandla e-FOREGENE anelungelo lobunikazi be-Taq enzyme( ilungelo lobunikazi eligunyaziwe: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), i-patent 6894926 (Japan)).I-enzyme yayo ye-Taq isebenzisa ubuchwepheshe bokuhlanganiswa kabusha kwe-DNA ukuze ihlanganise kabusha isifunda sesakhiwo esibophezelayo se-DNA ye-archaeal nucleic acid ebopha iphrotheni ene-Taq DNA polymerase engazweli ukushisa ukuze kwakhiwe isifanekiso se-DNA esinobudlelwane obandayo.I-Taq ethuthukisiwe eshisayo egcina imisebenzi ehlukahlukene ye-DNA polymerase yasekuqaleni.Le enzyme ye-Taq ingabophezela kusifanekiso se-DNA futhi iqalise ukuhlanganiswa kwe-DNA ezifanekisweni zokuhlushwa eziphansi nasezindaweni eziyinkimbinkimbi.
For isibonelo,itkulula njengokungeza kwakhoiseli,isitshalo (iqabunga elincane, impande noma imbewu) noma isampula yesilwanebuffer, futhi wengeza okuhlukilemaster mix withethizeama-primers kumashubhu e-PCR futhi ayisebenzise ngebhayisikili eshisayo.Ukusebenza kahle kwekhithi kulungele ukuhlaziya isampula enkulu lapho isikhathi sokulondoloza sibaluleke kakhulu.
Isistimu yokusabela ye-PCR equkethe i-UNG enzyme ne-dUTP ikhethwa ngokuvumelana nezidingo zokuhlola, ezingagwema ngempumelelo ukungcola okubangelwa imikhiqizo yokukhulisa i-PCR.
I-PCR eqondile | Indlela Yendabuko (RNA/DNA Purification+Conventional PCR) | ||
Ukusetshenziswa Kwempahla | Ukusetshenziswa Kwezicubu | Ngaphansi | Kakhulu |
Ukulungiselela isifanekiso | Uhlobo Lwezinto | Akukho ukugaya okudingekayo | Amasampula okugaya |
Ukusebenza | Uhlobo lweshubhu eyodwa, imizuzu eyi-10 | Ukusebenza okuyinkimbinkimbi, 1h | |
I-Flux | 1-96 | 1-24 | |
Ukusabela kwe-PCR | Isistimu yokusabela | Kulungiselelwe imiksi engu-2×PCR | dNTP,MgCl2,10×PCR Buffer,Taq Enzyme |
01 Ukuhlonzwa kwama-microorganisms e-pathogenic
02 Ukuhlonza okuguqulwe ngofuzo, i-genotyping
03 Ukutholwa kwe-Multiplex PCR / SNP / PCR-RFLP
04Ukulandelana/ukuhlanganisa
Igama Lomkhiqizo | Imininingwane | Ikhathalogi Inombolo | Izimo Zokugcina |
QuickEasyTMI-Cell Direct RT-qPCR Kit–SYBR Green I/Taqman | 200T | I-DRT-01011 /DRT-01021 | Ingxenye I 4℃,Ingxenye II -20℃ |
1000T | I-DRT-01012 /DRT-01022 |
Igama Lomkhiqizo | Imininingwane | Ikhathalogi Inombolo | Izimo Zokugcina |
Igazi SuperDirectTMIkhithi ye-PCR (EDTA) | 200 × 20μl rxns | I-TP-04111 | -20 ℃ |
2000 × 20μl rxns | TP-04113 | ||
Igazi SuperDirectTMI-PCR Kit(EDTA) -UNG | 200 × 20μl rxns | I-TP-04121 | -20 ℃ |
2000 × 20μl rxns | TP-04123 | ||
Igazi SuperDirectTMPCR Kit (Heparin) | 200 × 20μl rxns | TP-04211 | -20 ℃ |
2000 × 20μl rxns | TP-04213 | ||
Igazi SuperDirectTMPCR Kit(Heparin) -UNG | 200 × 20μl rxns | TP-04221 | -20 ℃ |
2000 × 20μl rxns | TP-04223 |
Igama Lomkhiqizo | Imininingwane | Ikhathalogi Inombolo | Izimo Zokugcina |
I-Animal Tissue Direct PCR Kit | 200 × 20μl rxns | I-TP-01111 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | I-TP-01113 | ||
I-Animal Tissue Direct PCR Kit(65℃ lysis) -UNG | 200 × 20μl rxns | I-TP-01121 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | I-TP-01123 | ||
I-Mouse Tail SuperDirectTM PCR Kit(izinga lokushisa legumbi) | 200 × 20μl rxns | I-TP-01311 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | I-TP-01313 | ||
I-Mouse Tail Direct PCR Kit (65℃ lysis) | 200 × 20μl rxns | I-TP-01331 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-01333 | ||
I-Mouse Tail Direct PCR Kit(65℃ lysis) -UNG | 200 × 20μl rxns | I-TP-01341 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | I-TP-01343 | ||
I-Zebra Fish Direct PCR Kit(65℃ lysis) | 200 × 20μl rxns | I-TP-01411 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | I-TP-01413 | ||
I-Zebra Fish Direct PCR Kit(65℃ lysis) -UNG | 200 × 20μl rxns | I-TP-01421 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | I-TP-01423 |
Igama Lomkhiqizo | Imininingwane | Ikhathalogi Inombolo | Izimo Zokugcina |
Plant Leaf Direct PCR Kit | 200 × 20μl rxns | I-TP-02111 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | I-TP-02113 | ||
Plant Leaf Direct PCR Kit -UNG | 200 × 20μl rxns | I-TP-02121 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-02123 | ||
Plant Leaf Direct PCR Plus Kit (Isitshalo se-polysaccharide polyphenol) | 200 × 20μl rxns | I-TP-02131 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-02133 | ||
Plant Leaf Direct PCR Plus Kit -UNG (Polysaccharide polyphenol plant) | 200 × 20μl rxns | I-TP-02141 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-02143 | ||
Plant Seed Direct PCR Kit I | 200 × 20μl rxns | I-TP-03111 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-03113 | ||
Plant Seed Direct PCR Kit I -UNG | 200 × 20μl rxns | I-TP-03121 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-03123 | ||
Plant Seed Direct PCR Kit II | 200 × 20μl rxns | TP-03131 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-03133 | ||
Plant Seed Direct PCR Kit II -UNG | 200 × 20μl rxns | I-TP-03141 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-03143 | ||
Plant Seed Direct PCR Plus Kit I | 200 × 20μl rxns | TP-03151 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-03153 | ||
Plant Seed Direct PCR Plus Kit I -UNG | 200 × 20μl rxns | I-TP-03161 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | I-TP-03163 | ||
Plant Seed Direct PCR Plus Kit II | 200 × 20μl rxns | TP-03171 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-03173 | ||
Plant Seed Direct PCR Plus Kit II -UNG | 200 × 20μl rxns | I-TP-03181 | Ingxenye I 4℃,Ingxenye II -20℃ |
2000 × 20μl rxns | TP-03183 |
*Ikhithi ye-Covid Nucleic acid RT-PCR, ye-ORF1ab, N, E,S, nezinye eziguquguqukayo, ezifana nokuhluka kwe-Delta, njll.
96Rxns/ikhithi
Izinzuzo:
-Qedela ukuhlolwa esikhathini esingangehora elingu-1
-Asikho isidingo sokukhishwa kwe-RNA nokuzihlukanisa
-Ukucaciswa okuphezulu nokuzwela
Sithumele i-imeyili ukuze uthole ulwazi olwengeziwe:
E:overseas@foregene.com