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I-PCR eqondile

Iyini i-Direct PCR

I-Direct PCR iyindlela yokukhulisa i-DNA ngqo kusukaigazi, izicubu zezilwane,umsila wegundane,iseli,indlebe yegundane,idube izinhlanzi, amaqanda ezinhlanzi,tshala amaqabunga,imbewunoma elinye isampula lethishu yebhayoloji yoqobo ngaphandle kokwenza ukuhlukaniswa kwe-DNA nezinyathelo zokuyihlanza.Indlela ye-PCR eqondile inganciphisa kakhulu isikhathi sokuhlola, kanye nezindleko kumaphrojekthi we-genotyping kanye nenani eliphezulu.Iphinde inikeze inketho engcono uma ubhekene nezinselele zokukhulisa inani elincane kakhulu lesampula lapho isinyathelo sokuhlanza singase siholele ekulahlekeni kwesampula.

I-ForEGENE's PCR Mix inokumelana nokucindezeleka okuqinile, ngenxa yamandla e-FOREGENE anelungelo lobunikazi be-Taq enzyme( ilungelo lobunikazi eligunyaziwe: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), i-patent 6894926 (Japan)).I-enzyme yayo ye-Taq isebenzisa ubuchwepheshe bokuhlanganiswa kabusha kwe-DNA ukuze ihlanganise kabusha isifunda sesakhiwo esibophezelayo se-DNA ye-archaeal nucleic acid ebopha iphrotheni ene-Taq DNA polymerase engazweli ukushisa ukuze kwakhiwe isifanekiso se-DNA esinobudlelwane obandayo.I-Taq ethuthukisiwe eshisayo egcina imisebenzi ehlukahlukene ye-DNA polymerase yasekuqaleni.Le enzyme ye-Taq ingabophezela kusifanekiso se-DNA futhi iqalise ukuhlanganiswa kwe-DNA ezifanekisweni zokuhlushwa eziphansi nasezindaweni eziyinkimbinkimbi.

For isibonelo,itkulula njengokungeza kwakhoiseli,isitshalo (iqabunga elincane, impande noma imbewu) noma isampula yesilwanebuffer, futhi wengeza okuhlukilemaster mix withethizeama-primers kumashubhu e-PCR futhi ayisebenzise ngebhayisikili eshisayo.Ukusebenza kahle kwekhithi kulungele ukuhlaziya isampula enkulu lapho isikhathi sokulondoloza sibaluleke kakhulu.

Isistimu yokusabela ye-PCR equkethe i-UNG enzyme ne-dUTP ikhethwa ngokuvumelana nezidingo zokuhlola, ezingagwema ngempumelelo ukungcola okubangelwa imikhiqizo yokukhulisa i-PCR.

Izinzuzo kanye nezicelo ze-Direct PCR

idada (5)
idada (1)
Izinzuzo ze-Direct PCR
Isicelo
Izinzuzo ze-Direct PCR
    I-PCR eqondile Indlela Yendabuko
(RNA/DNA Purification+Conventional PCR)
Ukusetshenziswa Kwempahla Ukusetshenziswa Kwezicubu Ngaphansi Kakhulu
Ukulungiselela isifanekiso Uhlobo Lwezinto Akukho ukugaya okudingekayo Amasampula okugaya
Ukusebenza Uhlobo lweshubhu eyodwa, imizuzu eyi-10 Ukusebenza okuyinkimbinkimbi, 1h
I-Flux 1-96 1-24
Ukusabela kwe-PCR Isistimu yokusabela Kulungiselelwe imiksi engu-2×PCR dNTP,MgCl2,10×PCR Buffer,Taq Enzyme
Isicelo

01 Ukuhlonzwa kwama-microorganisms e-pathogenic

02 Ukuhlonza okuguqulwe ngofuzo, i-genotyping

03 Ukutholwa kwe-Multiplex PCR / SNP / PCR-RFLP

04Ukulandelana/ukuhlanganisa

Imikhiqizo yekhithi ye-Foregene Direct PCR

I-Cell Direct RT-qPCR Kits
I-Blood Direct PCR Kits
I-Animal Direct PCR Kits
Plant Direct PCR Kits
I-IVD Kits
I-Cell Direct RT-qPCR Kits
Igama Lomkhiqizo Imininingwane Ikhathalogi
Inombolo
Izimo Zokugcina
QuickEasyTMI-Cell Direct RT-qPCR Kit–SYBR Green I/Taqman 200T I-DRT-01011
/DRT-01021
Ingxenye I 4℃,Ingxenye II -20℃
1000T I-DRT-01012
/DRT-01022
I-Blood Direct PCR Kits
Igama Lomkhiqizo Imininingwane Ikhathalogi
Inombolo
Izimo Zokugcina
Igazi SuperDirectTMIkhithi ye-PCR (EDTA) 200 × 20μl rxns I-TP-04111 -20 ℃
2000 × 20μl rxns TP-04113
Igazi SuperDirectTMI-PCR Kit(EDTA) -UNG 200 × 20μl rxns I-TP-04121 -20 ℃
2000 × 20μl rxns TP-04123
Igazi SuperDirectTMPCR Kit (Heparin) 200 × 20μl rxns TP-04211 -20 ℃
2000 × 20μl rxns TP-04213
Igazi SuperDirectTMPCR Kit(Heparin) -UNG 200 × 20μl rxns TP-04221 -20 ℃
2000 × 20μl rxns TP-04223
I-Animal Direct PCR Kits
Igama Lomkhiqizo Imininingwane Ikhathalogi
Inombolo
Izimo Zokugcina
I-Animal Tissue Direct PCR Kit 200 × 20μl rxns I-TP-01111 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns I-TP-01113
I-Animal Tissue Direct PCR Kit(65℃ lysis) -UNG 200 × 20μl rxns I-TP-01121 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns I-TP-01123
I-Mouse Tail SuperDirectTM PCR Kit(izinga lokushisa legumbi) 200 × 20μl rxns I-TP-01311 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns I-TP-01313  
I-Mouse Tail Direct PCR Kit
(65℃ lysis)
200 × 20μl rxns I-TP-01331 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-01333
I-Mouse Tail Direct PCR Kit(65℃ lysis) -UNG 200 × 20μl rxns I-TP-01341 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns I-TP-01343
I-Zebra Fish Direct PCR Kit(65℃ lysis) 200 × 20μl rxns I-TP-01411 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns I-TP-01413
I-Zebra Fish Direct PCR Kit(65℃ lysis) -UNG 200 × 20μl rxns I-TP-01421 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns I-TP-01423
Plant Direct PCR Kits
Igama Lomkhiqizo Imininingwane Ikhathalogi
Inombolo
Izimo Zokugcina
Plant Leaf Direct PCR Kit 200 × 20μl rxns I-TP-02111 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns I-TP-02113
Plant Leaf Direct PCR Kit -UNG 200 × 20μl rxns I-TP-02121 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-02123
Plant Leaf Direct PCR Plus Kit
(Isitshalo se-polysaccharide polyphenol)
200 × 20μl rxns I-TP-02131 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-02133
Plant Leaf Direct PCR Plus Kit -UNG (Polysaccharide polyphenol plant) 200 × 20μl rxns I-TP-02141 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-02143
Plant Seed Direct PCR Kit I 200 × 20μl rxns I-TP-03111 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-03113
Plant Seed Direct PCR Kit I -UNG 200 × 20μl rxns I-TP-03121 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-03123
Plant Seed Direct PCR Kit II 200 × 20μl rxns TP-03131 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-03133
Plant Seed Direct PCR Kit II -UNG 200 × 20μl rxns I-TP-03141 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-03143
Plant Seed Direct PCR Plus Kit I 200 × 20μl rxns TP-03151 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-03153
Plant Seed Direct PCR Plus Kit I -UNG 200 × 20μl rxns I-TP-03161 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns I-TP-03163
Plant Seed Direct PCR Plus Kit II 200 × 20μl rxns TP-03171 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-03173
Plant Seed Direct PCR Plus Kit II -UNG 200 × 20μl rxns I-TP-03181 Ingxenye I 4℃,Ingxenye II -20℃
2000 × 20μl rxns TP-03183

 

I-IVD Kits

*Ikhithi ye-Covid Nucleic acid RT-PCR, ye-ORF1ab, N, E,S, nezinye eziguquguqukayo, ezifana nokuhluka kwe-Delta, njll.

96Rxns/ikhithi

Izinzuzo:

-Qedela ukuhlolwa esikhathini esingangehora elingu-1

-Asikho isidingo sokukhishwa kwe-RNA nokuzihlukanisa

-Ukucaciswa okuphezulu nokuzwela

Imininingwane yekhithi yokuhlola ye-Covid chofoza lapha