I-PCR eqondile

Iyini i-Direct PCR

I-Direct PCR iyindlela yokukhulisa i-DNA ngqo kusukaigazi, izicubu zezilwane,umsila wegundane,iseli,indlebe yegundane,idube izinhlanzi, amaqanda ezinhlanzi,tshala amaqabunga,imbewunoma elinye isampula lethishu yebhayoloji yoqobo ngaphandle kokwenza ukuhlukaniswa kwe-DNA nezinyathelo zokuyihlanza.Indlela ye-PCR eqondile inganciphisa kakhulu isikhathi sokuhlola, kanye nezindleko kumaphrojekthi we-genotyping kanye nenani eliphezulu.Iphinde inikeze inketho engcono uma ubhekene nezinselele zokukhulisa inani elincane kakhulu lesampula lapho isinyathelo sokuhlanza singase siholele ekulahlekeni kwesampula.

I-ForEGENE's PCR Mix inamandla okumelana nengcindezi, ngenxa yamandla e-FOREGENE anelungelo lobunikazi be-Taq enzyme( ilungelo lobunikazi eligunyaziwe: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), ilungelo lobunikazi 6894926 (Japan)I-enzyme yayo ye-Taq isebenzisa ubuchwepheshe bokuhlanganiswa kabusha kwe-DNA ukuze kuhlanganiswe kabusha isifunda sesakhiwo esibophezelayo se-DNA ye-archaeal nucleic acid ebopha iphrotheni ene-Taq DNA polymerase engazweli ukushisa ukuze kwakhiwe isifanekiso se-DNA esinobudlelwane obandayo.I-Taq ethuthukisiwe eqala ukushisa egcina imisebenzi ehlukahlukene ye-DNA polymerase yasekuqaleni.Le enzyme ye-Taq ingabophezela kusifanekiso se-DNA futhi iqalise ukuhlanganiswa kwe-DNA ezifanekisweni zokuhlushwa eziphansi nasezindaweni eziyinkimbinkimbi.

For isibonelo,itkulula njengokungeza kwakhoiseli,isitshalo (iqabunga elincane, impande noma imbewu) noma isampula yesilwanebuffer, futhi wengeza okuhlukilemaster mix withethizeama-primers kumashubhu e-PCR futhi ayisebenzise ngomjikelezo oshisayo.Ukusebenza kahle kwekhithi kulungele ukuhlaziya isampula enkulu lapho isikhathi sokulondoloza sibaluleke kakhulu.

Isistimu yokusabela ye-PCR equkethe i-UNG enzyme kanye ne-dUTP ikhethwa ngokuya ngezidingo zokuhlola, ezingagwema ngempumelelo ukungcola okubangelwa imikhiqizo yokukhulisa i-PCR.

asdada (5)
asdada (1)

Izinzuzo zeDI-PCR eqondile

 

 

I-PCR eqondile

Indlela yendabuko

(I-RNA purification+ Coventional PCR)

Ukusetshenziswa kwezinto ezibonakalayo

Ukusetshenziswa kwezicubu

Ngaphansi

Okuningi

Ukulungiselela isifanekiso

Uhlobo lwezinto

Akukho ukugaya okudingekayo

Amasampula okugaya

Ukusebenza

Uhlobo lwe-tube eyodwa, i-10min

ukusebenza okuyinkimbinkimbi, 1h

Flux

1-96

1-24

Ukusabela kwe-PCR

uhlelo lokusabela

Kulungiselelwe imiksi engu-2×PCR

Dntp, MgCl2,10×PCR Buffer,Taq enzyme,

Aisicelo

01 Ukuhlonzwa kwama-microorganisms e-pathogenic

02 Ukuhlonza okuguqulwe ngofuzo, i-genotyping

03 Ukutholwa kwe-Multiplex PCR / SNP / PCR-RFLP

04Ukulandelana/ukuhlanganisa

Uchungechunge Lomkhiqizo

 

*Ikhithi ye-Covid Nucleic acid RT-PCR, ye-ORF1ab, N, E,S , nezinye eziguquguqukayo, ezifana nokuhluka kwe-Delta, njll.

96Rxns/ikhithi

Izinzuzo:

-Qedela ukuhlolwa esikhathini esingangehora elingu-1

-Asikho isidingo sokukhishwa kwe-RNA nokuzihlukanisa

-Ukucaciswa okuphezulu nokuzwela

Imininingwane yekhithi yokuhlola ye-Covid chofoza lapha

 

 

 

asdada (3)

Uchungechunge

Igama Lomkhiqizo

Imininingwane

Inombolo Yekhathalogi

Izimo Zokugcina

Uchungechunge oluqondile lwe-RT-qPCR

QuickEasyTMI-Cell Direct RT-qPCR Kit–SYBR Green I

200T

I-DRT-01011

Ingxenye I 4℃,Ingxenye II -20℃

1000T

I-DRT-01012

asdada (12)

 

Uchungechunge

Igama Lomkhiqizo

Imininingwane

Inombolo Yekhathalogi

Izimo Zokugcina

 

 

 

Igazi Direct PCR Series

Igazi SuperDirectTMI-PCR Kit (EDTA)

200 × 20μl rxns

TP-04111

-20 ℃

2000 × 20μl rxns

TP-04113

Igazi SuperDirectTMI-PCR Kit(EDTA)-UNG

200 × 20μl rxns

TP-04121

-20 ℃

2000 × 20μl rxns

TP-04123

Igazi SuperDirectTMIkhithi ye-PCR
(iHeparin)

200 × 20μl rxns

TP-04211

-20 ℃

2000 × 20μl rxns

TP-04213

Igazi SuperDirectTMPCR Kit(Heparin) -UNG

200 × 20μl rxns

TP-04221

-20 ℃

2000 × 20μl rxns

TP-04223

 

asdada (7)
asdada (4)

 

Series

Igama Lomkhiqizo

Imininingwane

Inombolo Yekhathalogi

Izimo Zokugcina

Animal Direct PCR Series

I-Animal Tissue Direct PCR Kit(65℃ lysis)

200 × 20μl rxns

I-TP-01111

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

I-TP-01113

I-Animal Tissue Direct PCR Kit(65℃ lysis) -UNG

200 × 20μl rxns

I-TP-01121

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

I-TP-01123

Igundane Umsila SuperDirectTMIkhithi ye-PCR
(izinga lokushisa legumbi)

200 × 20μl rxns

I-TP-01311

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

I-TP-01313

I-Mouse Tail Direct PCR Kit(65℃ lysis)

200 × 20μl rxns

I-TP-01331

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-01333

I-Mouse Tail Direct PCR Kit(65℃ lysis) - UNG

200 × 20μl rxns

I-TP-01341

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

I-TP-01343

I-Zebra Fish Direct PCR Kit(65℃ lysis)

200 × 20μl rxns

I-TP-01411

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

I-TP-01413

I-Zebra Fish Direct PCR Kit(65℃ lysis) - UNG

200 × 20μl rxns

I-TP-01421

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-01423

asdada (8)
asdada (10)
asdada (9)
asdada (11)

Uchungechunge

Igama Lomkhiqizo

Imininingwane

Inombolo Yekhathalogi

Izimo Zokugcina

Plant Direct PCR Series

Plant Leaf Direct PCR Kit

200 × 20μl rxns

I-TP-02111

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-02113

Plant Leaf Direct PCR Kit -UNG

200 × 20μl rxns

I-TP-02121

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-02123

Plant Leaf Direct PCR Plus Kit
(Isitshalo se-polysaccharide polyphenol)

200 × 20μl rxns

TP-02131

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-02133

Plant Leaf Direct PCR Plus Kit -UNG
(Isitshalo se-polysaccharide polyphenol)

200 × 20μl rxns

I-TP-02141

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-02143

Plant Seed Direct PCR Kit I

200 × 20μl rxns

I-TP-03111

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-03113

Plant Seed Direct PCR Kit I -UNG

200 × 20μl rxns

TP-03121

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-03123

Plant Seed Direct PCR Kit II

200 × 20μl rxns

TP-03131

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-03133

Plant Seed Direct PCR Kit II -UNG

200 × 20μl rxns

TP-03141

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-03143

Plant Seed Direct PCR Plus Kit I(Polysaccharide polyphenol plant)

200 × 20μl rxns

TP-03151

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-03153

Plant Seed Direct PCR Plus Kit I - UNG(Polysaccharide polyphenol plant)

200 × 20μl rxns

I-TP-03161

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-03163

Plant Seed Direct PCR Plus Kit II

200 × 20μl rxns

TP-03171

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-03173

Plant Seed Direct PCR Plus Kit II - UNG(Polysaccharide polyphenol plant)

200 × 20μl rxns

I-TP-03181

Ingxenye I 4℃,Ingxenye II -20℃

2000 × 20μl rxns

TP-03183