I-China Mouse Tail I-SuperDirect PCR Kit Direct PCR Lysis Reagent(Umsila Wegundane)(ye-Genotyping) Umkhiqizi Nomhlinzeki |I-Foregene

I-Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Umsila Wegundane)(ye-Genotyping)

I-Mouse Tail I-SuperDirect PCR Kit Direct PCR Lysis Reagent(Umsila Wegundane)(ye-Genotyping) Isithombe Esifakiwe

Incazelo yekhithi:

Sebenzisa ngokuqondile i-lysate yomsila wegundane njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.

◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.

◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane

Le khithi isebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze ikhulule ngokushesha i-DNA ye-genomic kusuka kumsila wegundane namasampula endlebe yegundane ukuze kutholakale ukusabela kwe-PCR, ngakho ifaneleka ngokukhethekile ukuhlolwa kofuzo okukhulu.

Inqubo yokukhipha i-genomic DNA ku-lysis buffer iqediwephakathi kwemizuzu eyi-10-30kweUkushisa kwegumbi (20-25).°C).Akukho ukushisisa okudingekayo, futhi ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA azidingeki.Amanani omkhondo akhishiwe we-DNA angasetshenziswa njengesifanekiso sokusabela kwe-PCR.

2×M-PCR Kulula^TMI-Mix inokubekezelela okuqinile kwe-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ukuze ihlolwe njengesifanekiso sokukhulisa kahle nokuqondile.Lesi sikhungo siqukethe i-Foregene D-Taq DNAPolymerase, i-dNTPs, i-MgCl₂, isigcinalwazi sokusabela, i-PCR optimizer nesiqinisi.

I-D-Taq DNA polymerase iyi-polymerase ye-DNA ethuthukiswe ngokukhethekile yi-Foregene ukuze kutholakale ukusabela okuqondile kwe-PCR.I-D-Taq DNA polymerase inokubekezelela okunamandla ezinhlobonhlobo ze-PCR reaction inhibitors, futhi ingakhulisa kahle amanani omkhondo we-DNA ezinhlelweni ezihlukene zokusabela eziyinkimbinkimbi, futhi isivinini sokukhulisa singafinyelela ku-2Kb/min, okulungele ikakhulukazi ukusabela kwe-Direct PCR.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I	I-Buffer NP
	I-Foregene Protease Plus I
	I-6× I-DNA Yokulayisha Ibhafa
Ingxenye II	2× M PCR EasyTM Mix
Iziyalezo

Izici&izinzuzo

■Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

■ Isidingo sesampula sincane, thatha nje imisila yegundane (noma izindlebe zegundane ezinobubanzi obungu-2-5mm) ngaphezu kuka-2mm.

■ Kulula ukuyisebenzisa, ingafakwa i-lysed ngaphandle kokushisisa ukuze yenze i-protease ingasebenzi, futhi umkhiqizo owenziwe nge-lysed ungaba ngaphansi kokusabela kwe-PCR ngokuqondile.

■ I-Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) ingasetshenziswa ukukhipha i-DNA ye-genomic emsileni wegundane ocutshunguliwe.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

Imingcele yekhithi

Isicelo: Umsila wegundane, izindlebe zegundane, imisipha nezinye izicubu.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Ukugeleza komsebenzi

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer FP ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

I-Reagent Foregene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-Reagent 2×PCR EasyTM Mix, uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).

Okwedlule: I-Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(i-zebrafish)

Olandelayo: I-Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Umsila Wegundane)(ye-Genotyping)

Bhala umyalezo wakho lapha futhi usithumelele wona