Izindaba Zezimboni
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Mayelana namasosha omzimba e-monoclonal, i-athikili enhle ongeke uyiphuthele!
Ama-antibodies, abizwa nangokuthi ama-immunoglobulins (Ig), angama-glycoprotein abopha ngokuqondile kuma-antigen.Ukulungiswa okujwayelekile kwama-antibody kukhiqizwa ngokugoma izilwane nokuqoqa i-antiserum.Ngakho-ke, i-antiserum ngokuvamile iqukethe amasosha omzimba ngokumelene namanye ama-antigen angahlobene namanye amaprotheni ...Funda kabanzi -
Izidakamizwa ze-Nucleic acid sezingene enkathini yegolide, yibuphi ubuchwepheshe obubalulekile obudinga ukuxazululwa ngokuphuthumayo?
Umthombo: I-Medical Micro Ngemuva kokugqashuka kwe-COVID-19, imigomo emibili ye-mRNA yagunyazwa ngokushesha ukuthi ikhangiswe, osekudonse ukunakwa okwengeziwe ekwakhiweni kwezidakamizwa ze-nucleic acid.Eminyakeni yamuva nje, inani lezidakamizwa ze-nucleic acid ezinamandla okuba izidakamizwa ze-blockbuster ziye zashicilela ...Funda kabanzi -
Ukuhlaziywa Kwezinkomba Zokuqinisekisa zama-Primer Probes Esikhathini sokuqala sama-PCR Reagents
Ukuqinisekisa ukusebenza kwama-primer nama-probes esigabeni sokuqala sama-reagents e-PCR kanye nokuthola izimo zokusabela ezifaneleka kakhulu kuyizimfuneko zokuqinisekisa ukuqhubeka kahle kokuhlolwa okusemthethweni.Ngakho-ke sidinga kanjani ukuqinisekisa uphenyo lwe-primer ekuqaleni ...Funda kabanzi -
Izibonelo zamaphuzu olwazi okufanele abahloli bawabone
Ukuhlolwa kwaselabhorethri kuqala ngokuqoqwa kwesampula, futhi ukuqoqwa kwesampula kulula kakhulu ukungakunaki.Into ebaluleke kakhulu ekuqoqweni kwesampula ukukhetha uhlobo olulungile lwesampula, ukusebenzisa amathuluzi amasampula afanelekile, nokwenza ukuthutha nokucubungula okuphusile.I.Sample type Common sam...Funda kabanzi -
Isixazululo esiphezulu sokungcoliswa kwe-aerosol ye-nucleic acid
Izindlela ze-PCR kanye nokungcoliswa kwe-aerosol ye-nucleic acid kumalabhoratri okuhlola i-nucleic acid kufana nezinhlangothi ezimbili zohlamvu lwemali.Singakhetha kuphela ukuba nayo noma cha, kodwa ngeke sikhethe ukuthi siyayifuna noma siyayisebenzisa.1. Ukuhlolwa kwesikhiphi se-DNA Ukufeza ukususwa kwendawo...Funda kabanzi -
Ukuhlonza ngokushesha izitshalo ezishintshashintshayo
Njengentsha elabhorethri, akuwona umsebenzi omuhle ukuhlola izitshalo ezinhle esixukwini sezitshalo ezinezinga eliphansi lokuguqulwa.Okokuqala, i-DNA kufanele ikhishwe enanini elikhulu lamasampuli ngalinye ngalinye, bese kuthi ufuzo lwangaphandle luzotholwa yi-PCR.Nokho, imiphumela ivamise ukungabi nalutho...Funda kabanzi -
Ukuchazwa okuphelele kwamaphuzu abalulekile okuhlolwa kwe-SARS-CoV-2 nucleic acid, kungani kukhona okungalungile okungamanga kanye nokuphinda kuhlolwe okuhle?
Esigabeni sokuqala sokuqubuka kwalesi sifo, ngenxa yentuthuko esheshayo, ukutholwa ngokushesha kweziguli ezisolwayo kuwukhiye wokuvimbela i-COVID-19.Amanye ama-reagents okutholwa kwe-nucleic acid agunyaziwe anesikhathi esifushane sokuthuthukiswa, futhi kunezinkinga ezifana nokuqinisekiswa kokusebenza okusheshayo, i-reaction enganele...Funda kabanzi -
Iyini i-SNP?Izihloko ze-Population Genetics
Izinhlamvu ezintathu ze-SNP zitholakala yonke indawo ocwaningweni lwezakhi zofuzo zabantu.Kungakhathalekile ukuthi kwenziwa ucwaningo ngezifo zomuntu, ukubekwa kwesimo sezitshalo, ukuvela kwezilwane kanye nemvelo yamangqamuzana, ama-SNP ayadingeka njengesisekelo.Kodwa-ke, uma ungenakho ukuqonda okujulile kofuzo lwesimanje...Funda kabanzi -
Ungakuthuthukisa kanjani ukusebenza kahle kokubhala okuhlanekezelwe kwe-LncRNA?
Izici ze-lncRNA: 1. Ama-lncRNA avame ukuba made, anenkulumo eguquguqukayo nezindlela ezihlukene zokuhlanganisa ngesikhathi sokuhlukaniswa;2. Uma kuqhathaniswa nofuzo lwekhodi, i-lncRNA ivamise ukuba ngaphansi;3. Iningi lama-lncRNA anokucaciswa okusobala kwesikhashana kanye nendawo kunqubo ...Funda kabanzi -
Izixazululo Ezine Eziyinhloko Zokulawula Ukungcola Komkhiqizo we-PCR
1:Miselela okokusebenza kokuhlola ngesikhathi sokusethwa kwesikhathi (NTC) isilawuli esinegethivu bese ukuphinda izikhathi eziningi.Uma sekutholakele ukuthi kukhona ukungcoliswa komkhiqizo we-PCR elabhorethri, buyisela zonke izinto zokuhlola ngesikhathi.Okufana nokuthi: hlambulula kabusha futhi ulungise iziqalo, phinda ugcwalise ithiphu le-pipette, E...Funda kabanzi -
Ama-enzyme amabili e-RT-PCR asebenza kabili
Imibhalo yendabuko ehlanekezelwe ayikwazi ukubekezelela izinga lokushisa eliphezulu (izinga lokushisa elilungile lomsebenzi we-MMLV ngu-37-50°C, futhi i-AMV ingu-42-60°C).I-RNA yegciwane eyinkimbinkimbi kakhulu ayikwazi ukuhlehliswa ngokuyimpumelelo ibhalwe ku-cDNA emazingeni okushisa aphansi, okuholela ekusebenzeni kahle kokutholwa Ukuncipha.Tra...Funda kabanzi -
Ubuchwepheshe be-nucleic acid isothermal amplification
I-PCR ubuchwepheshe bokukhulisa i-nucleic acid obusetshenziswa kakhulu futhi busetshenziswa kakhulu ngenxa yokuzwela nokucaciswa kwayo.Kodwa-ke, i-PCR idinga ukukhishwa kwe-thermal okuphindaphindiwe futhi ayikwazi ukususa imikhawulo yokuthembela kumathuluzi nemishini, ekhawulela ukusetshenziswa kwayo emitholampilo ...Funda kabanzi
