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I-RT-PCR Easyᵀᴹ I(Isinyathelo esisodwa)
◮Ikhithi yesinyathelo esisodwa yenza ukuba ukuloba okuhlanekezelwe kanye ne-PCR kwenziwe ngeshubhu efanayo.Idinga kuphela ukwengeza isifanekiso se-RNA, iziqalisi ze-PCR ezithile kanye ne-ddH Yamahhala ye-RNase2O.
◮Ukuhlaziywa komthamo wesikhathi sangempela se-RNA kungenziwa ngokushesha nangokunembile.
◮Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
◮Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela kokutholwa okuphezulu, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.
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I-Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits from Mouse Tail
Ikhithi eshesha kakhulu emhlabeni yokukhipha i-genomic DNA yomsila wegundane, engakhipha i-DNA yekhwalithi ephezulu emsileni wegundane lingakapheli ihora elingu-1.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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Ikhithi ye-Bacterial DNA Isolation Kit I-Bacterial Genomic DNA Extraction Purification Kits
Hlanza ngokushesha i-DNA ye-genomic yekhwalithi ephezulu kubhaktheriya esigabeni sokukhula se-logarithmic.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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I-ATM/CSP11 I-Dual Color Probe
Ngokomgomo wokumataniswa okuhambisanayo kwezisekelo ze-DNA, i-ATM orange probe kanye ne-CSP11 eluhlaza okotshani kwasetshenziswa ukuze kuhlanganiswe nokulandelana okuqondiwe kwe-DNA ku-nucleus, kanye nolwazi lwesimo sofuzo ku-nucleus lwabonwa futhi lwahlaziywa ngaphansi kwesibonakhulu se-fluorescence.
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I-ERG1/TERT I-Dual Color Probe
I-Fluorescence in situ hybridization (FISH) isuselwe kumgomo wokumataniswa okuhambisanayo kwezisekelo ze-DNA, kanye nokubonwa kwamasiginali we-hybridization we-DNA probes enelebuli ye-fluorescence nokulandelana kwayo okuqondiwe kwe-DNA ku-nucleus yeseli ngaphansi kwesibonakhulu se-fluorescence.
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Ikhithi yokukhipha i-DNA/RNA (Ubuhlalu obuzibuthe)
- Yonke inqubo yokuzihlukanisa iphephile futhi ifanelekile
- I-DNA yamahhala yeseli ekhishiwe inesivuno esikhulu, ukuhlanzeka okuphezulu kanye nekhwalithi ethembekile
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20q12/20q13.33 I-Dual Color Probe
Ngokomgomo wokumataniswa okuhambisanayo kwezisekelo ze-DNA, uphenyo olusawolintshi lwe-20q12 (D20S108) kanye ne-20q33.3 eluhlaza okotshani kwasetshenziswa ukuze kuhlanganiswe nokulandelana kwethagethi ye-DNA ku-nucleus, kanye nolwazi lwesimo sofuzo ku-nucleus lwabonwa futhi lwahlaziywa ngaphansi kwe-fluorescence microscope.
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p53/D13S319 Ukuhlolwa Kombala Okabili
I-Fluorescence in situ hybridization (FISH) isuselwe kumgomo wokumataniswa okuhambisanayo kwezisekelo ze-DNA, kanye nokubonwa kwamasiginali we-hybridization we-DNA probes enelebuli ye-fluorescence nokulandelana kwayo okuqondiwe kwe-DNA ku-nucleus yeseli ngaphansi kwesibonakhulu se-fluorescence.
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I-EndoFree Maxi Plasmid Kit (Ikholomu ye-Spin)
Yonke inqubo yokukhipha ithatha ihora elingu-1 kuphela, iqinisekisa ukusebenza okulula nokusheshayo.
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I-MALT1 Dual Color Break Apart probe
Ngokomgomo wokumatanisa okuhambisanayo kwesisekelo se-DNA, i-MALT1 i-orange-red probe kanye ne-MALT1 eluhlaza okotshani kwasetshenziswa ukuze kuhlanganiswe nokulandelana okuqondiwe kwe-DNA ku-nucleus, kanye nolwazi lwesimo sofuzo ku-nucleus lwabonwa futhi lwahlaziywa ngaphansi kwesibonakhulu se-fluorescence.
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I-D7S522/CSP7 I-Dual Color Probe
I-Fluorescence in situ hybridization (FISH) isuselwe kumgomo wokumataniswa okuhambisanayo kwezisekelo ze-DNA, kanye nokubonwa kwamasiginali we-hybridization we-DNA probes enelebuli ye-fluorescence nokulandelana kwayo okuqondiwe kwe-DNA ku-nucleus yeseli ngaphansi kwesibonakhulu se-fluorescence.
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I-DAPI Counterstain
Ingxenye eyinhloko yesisombululo se-DAPI sokubuyisela kabusha i-4-phenylindole, engangena ku-membrane yeseli futhi ibophe ngokuqinile ku-DNA.
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