Uchungechunge oluqondile lwe-RT-qPCR

I-PCR eqondile

Iyini i-Direct PCR

I-Direct PCR iyindlela yokukhulisa i-DNA ngokuqondile egazini, izicubu zesilwane, umsila wegundane, iseli, indlebe yegundane, idube inhlanzi, amaqanda ezinhlanzi, amaqabunga ezitshalo, imbewu noma esinye isampula sokuqala sezicubu zebhayoloji ngaphandle kokwenza ukuhlukaniswa kwe-DNA kanye nezinyathelo zokuhlanza.Indlela ye-PCR eqondile inganciphisa kakhulu isikhathi sokuhlola, kanye nezindleko kumaphrojekthi we-genotyping kanye nenani eliphezulu.Iphinde inikeze inketho engcono uma ubhekene nezinselele zokukhulisa inani elincane kakhulu lesampula lapho isinyathelo sokuhlanza singase siholele ekulahlekeni kwesampula.

I-ForEGENE's PCR Mix inokumelana nokucindezeleka okuqinile, ngenxa yamandla e-FOREGENE anelungelo lobunikazi be-Taq enzyme( ilungelo lobunikazi eligunyaziwe: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), i-patent 6894926 (Japan)).I-enzyme yayo ye-Taq isebenzisa ubuchwepheshe bokuhlanganiswa kabusha kwe-DNA ukuze ihlanganise kabusha isifunda sesakhiwo esibophezelayo se-DNA ye-archaeal nucleic acid ebopha iphrotheni ene-Taq DNA polymerase engazweli ukushisa ukuze kwakhiwe isifanekiso se-DNA esinobudlelwane obandayo.I-Taq ethuthukisiwe eshisayo egcina imisebenzi ehlukahlukene ye-DNA polymerase yasekuqaleni.Le enzyme ye-Taq ingabophezela kusifanekiso se-DNA futhi iqalise ukuhlanganiswa kwe-DNA ezifanekisweni zokuhlushwa eziphansi nasezindaweni eziyinkimbinkimbi.

Isibonelo, kulula njengokwengeza iseli yakho, isitshalo (iqabunga elincane, impande noma imbewu) noma isampula yesilwane ku-buffer, futhi wengeze ukuxuba okuyinhloko okuyingqayizivele nama-primers athile kumashubhu e-PCR nokuwusebenzisa ngomjikelezo oshisayo.Ukusebenza kahle kwekhithi kulungele ukuhlaziya isampula enkulu lapho isikhathi sokulondoloza sibaluleke kakhulu.

Isistimu yokusabela ye-PCR equkethe i-UNG enzyme ne-dUTP ikhethwa ngokuvumelana nezidingo zokuhlola, ezingagwema ngempumelelo ukungcola okubangelwa imikhiqizo yokukhulisa i-PCR.

Izinzuzo zeDI-PCR eqondile

		I-PCR eqondile	Indlela yendabuko (I-RNA purification+ Coventional PCR)
Ukusetshenziswa kwezinto	Ukusetshenziswa kwezicubu	Ngaphansi	Kakhulu
Ukulungiselela isifanekiso	Uhlobo lwezinto	Akukho ukugaya okudingekayo	Amasampula okugaya
	Ukusebenza	Uhlobo lweshubhu eyodwa, 10min	ukusebenza okuyinkimbinkimbi, 1h
	I-Flux	1-96	1-24
Ukusabela kwe-PCR	uhlelo lokusabela	Kulungiselelwe imiksi engu-2×PCR	Dntp, MgCl₂,10×PCR Buffer,Taq enzyme,

Aisicelo

01 Ukuhlonzwa kwama-microorganisms e-pathogenic

02 Ukuhlonza okuguqulwe ngofuzo, i-genotyping

03 Ukutholwa kwe-Multiplex PCR / SNP / PCR-RFLP

04Ukulandelana/ukuhlanganisa

Uchungechunge Lomkhiqizo

Uchungechunge	Igama Lomkhiqizo	Imininingwane	Inombolo Yekhathalogi	Izimo Zokugcina
Uchungechunge oluqondile lwe-RT-qPCR	QuickEasy^TMI-Cell Direct RT-qPCR Kit–SYBR Green I	200T	I-DRT-01011	Ingxenye I 4℃，Ingxenye II -20℃
Uchungechunge oluqondile lwe-RT-qPCR	QuickEasy^TMI-Cell Direct RT-qPCR Kit–SYBR Green I	1000T	I-DRT-01012	Ingxenye I 4℃，Ingxenye II -20℃

Uchungechunge	Igama Lomkhiqizo	Imininingwane	Inombolo Yekhathalogi	Izimo Zokugcina
Igazi Direct PCR Series	Igazi SuperDirect^TMIkhithi ye-PCR (EDTA)	200 × 20μl rxns	I-TP-04111	-20 ℃
		2000 × 20μl rxns	TP-04113
	Igazi SuperDirect^TMI-PCR Kit(EDTA)-UNG	200 × 20μl rxns	I-TP-04121	-20 ℃
		2000 × 20μl rxns	TP-04123
	Igazi SuperDirect^TMIkhithi ye-PCR (I-Heparin)	200 × 20μl rxns	TP-04211	-20 ℃
		2000 × 20μl rxns	TP-04213
	Igazi SuperDirect^TMPCR Kit(Heparin) -UNG	200 × 20μl rxns	TP-04221	-20 ℃
		2000 × 20μl rxns	TP-04223

Series	Igama Lomkhiqizo	Imininingwane	Inombolo Yekhathalogi	Izimo Zokugcina
Animal Direct PCR Series	I-Animal Tissue Direct PCR Kit(65℃ lysis)	200 × 20μl rxns	I-TP-01111	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	I-TP-01113
	I-Animal Tissue Direct PCR Kit(65℃ lysis) -UNG	200 × 20μl rxns	I-TP-01121	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	I-TP-01123
	Igundane Umsila SuperDirect^TMIkhithi ye-PCR (izinga lokushisa legumbi)	200 × 20μl rxns	I-TP-01311	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	I-TP-01313
	I-Mouse Tail Direct PCR Kit(65℃ lysis)	200 × 20μl rxns	I-TP-01331	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-01333
	I-Mouse Tail Direct PCR Kit(65℃ lysis) - UNG	200 × 20μl rxns	I-TP-01341	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	I-TP-01343
	I-Zebra Fish Direct PCR Kit(65℃ lysis)	200 × 20μl rxns	I-TP-01411	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	I-TP-01413
	I-Zebra Fish Direct PCR Kit(65℃ lysis) - UNG	200 × 20μl rxns	I-TP-01421	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	I-TP-01423

Uchungechunge	Igama Lomkhiqizo	Imininingwane	Inombolo Yekhathalogi	Izimo Zokugcina
Plant Direct PCR Series	Plant Leaf Direct PCR Kit	200 × 20μl rxns	I-TP-02111	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	I-TP-02113
	Plant Leaf Direct PCR Kit -UNG	200 × 20μl rxns	I-TP-02121	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-02123
	Plant Leaf Direct PCR Plus Kit (Isitshalo se-polysaccharide polyphenol)	200 × 20μl rxns	I-TP-02131	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-02133
	Plant Leaf Direct PCR Plus Kit -UNG (Isitshalo se-polysaccharide polyphenol)	200 × 20μl rxns	I-TP-02141	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-02143
	Plant Seed Direct PCR Kit I	200 × 20μl rxns	I-TP-03111	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-03113
	Plant Seed Direct PCR Kit I -UNG	200 × 20μl rxns	I-TP-03121	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-03123
	Plant Seed Direct PCR Kit II	200 × 20μl rxns	TP-03131	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-03133
	Plant Seed Direct PCR Kit II -UNG	200 × 20μl rxns	I-TP-03141	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-03143
	Plant Seed Direct PCR Plus Kit I(Polysaccharide polyphenol plant)	200 × 20μl rxns	TP-03151	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-03153
	Plant Seed Direct PCR Plus Kit I - UNG(Polysaccharide polyphenol plant)	200 × 20μl rxns	I-TP-03161	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	I-TP-03163
	Plant Seed Direct PCR Plus Kit II	200 × 20μl rxns	TP-03171	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-03173
	Plant Seed Direct PCR Plus Kit II - UNG(Polysaccharide polyphenol plant)	200 × 20μl rxns	I-TP-03181	Ingxenye I 4℃，Ingxenye II -20℃
		2000 × 20μl rxns	TP-03183