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Plant DNA Isolation Kit Genomic Plant DNA Purification Kits Reagents Protocol
Cat.No.DE-06111/06112/06113
Ukuhlanzwa kwe-DNA ye-genomic kusuka ezicutshini zezitshalo ezahlukahlukene.
Hlanza ngokushesha futhi uthole i-DNA ye-genomic yekhwalithi ephezulu kumasampula ezitshalo (okuhlanganisa ama-polysaccharides namasampuli ezitshalo ze-polyphenol).
◮Akukho ukungcola kwe-RNase
◮Isivinini esisheshayo
◮Kulula: Umsebenzi wokuhlanza ungaqedwa ngemizuzu engama-30.
◮Elula: Izinga lokushisa legumbi, i-4℃ centrifugation kanye nemvula ye-ethanol ye-DNA ayidingeki.
◮Ukuphepha: Ayikho i-reagent ye-organic esetshenziswayo.
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Ikhithi Yezicubu Zenyama Yezilwane I-DNA Isolation Kit DNA Extraction Kits from Animal Tissue Samples
Ukukhishwa okusheshayo nokuhlanzwa kwe-genomic DNA emithonjeni eminingi, njengezicubu zezilwane, amaseli, njll.
◮Isivinini esisheshayo:umsebenzi ophezulu kunama-protease afanayo futhi igaye amasampula ezicubu ngokushesha;
◮Kulula:ingaqedwa ngemizuzu engama-50.
◮Kulula:Kwenziwa ezingeni lokushisa legumbi.
◮Ukuphepha:akukho reagent ephilayo esetshenziswayo.
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Ikhithi encane ye-DNA yegazi
Hlanza ngokushesha i-genomic DNA yekhwalithi ephezulu egazini elinqanyuliwe (<1ml).
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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I-General Plasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits
Hlanza ngokushesha i-plasmid DNA yekhwalithi ephezulu kubhaktheriya eguquliwe ukuze uthole ukuhlolwa okujwayelekile kwebhayoloji yamangqamuzana njengokuguqulwa nokugaya kwe-enzyme.
◮Susa i-RNA ngaphandle kokwengeza i-RNase
◮Elula-i-centrifugation yenziwa ekamelweni lokushisa
◮Ngokushesha-ukuhlinzwa kungaqedwa ngemizuzu engama-20
◮Kuphephile-akukho reagent ephilayo esetshenzisiwe
◮Ukuhlanzeka okuphezulu—OD260/280≈1.7-1.9
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I-Blood DNA Midi Kit (1-5ml) I-Blood Isolation Midi Kits ye-DNA Egazini
Hlanza ngokushesha i-genomic DNA yekhwalithi ephezulu egazini elinqanyuliwe (1-5ml).
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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I-Buccal Swab/FTA Card DNA Isolation Kit Genomic DNA Extraction noma Ikhithi Yokuhlanzwa ku-Buccal Swabs
Hlanza ngokushesha i-DNA yezinga eliphezulu ye-genomic kusuka kumasampula e-buccal swab/FTA Card.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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I-Genomic DNA Micro Kit Ikhithi encane yeGenomic DNA
Hlanza ngokushesha amasampula amancane ukuze uthole i-genomic DNA yekhwalithi ephezulu.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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Ikhithi yokuhlukanisa i-DNA yamahhala
Ukuhlukaniswa kwe-cfDNA kumasampula e-plasmaInombolo yekhathalogi TQ01BT0050, TQ01BT0100 -
Ikhithi Yokuhlukanisa I-DNA Yamanzi I-DNA Isizinda kanye Nekhithi Yokuhlanza Yamanzi
Hlanza ngokushesha i-DNA ye-genomic yekhwalithi ephezulu kuma-microorganisms kumasampula amanzi ahlukahlukene.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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Ikhithi Yokuhlukanisa I-DNA Yesitulo I-DNA Extraction Amakhithi Okuhlanza Esitokisini
Hlanza ngokushesha futhi uthole i-DNA ye-genomic yekhwalithi ephezulu kumasampula ezihlalo ahlukahlukene.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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I-PCR Purification Kit PCR Clean-up System Ye-Ultra-pure DNA
Hlanza ngokushesha futhi uthole izingcezu ze-DNA zekhwalithi ephezulu ohlelweni lwe-PCR;uma ufuna ukuhlukanisa futhi uthole izingcezu ezithile ze-DNA, sicela ukhethe ikhithi yokutakula ijeli.
◮ Uhlu olubanzi lokutholwa kwe-DNA: Izingcezu ze-DNA ezimfishane njengo-60bp futhi ezinkulu njengo-10kb zingatholwa.
◮ Ukusebenza kahle kokutakula: Ukusebenza kahle kokutakula ngokuvamile kungaphezu kwama-80%, futhi okuphezulu kakhulu kungafinyelela ngaphezu kwama-95%.
◮ Isivinini esisheshayo:Kulula ukusebenza, ukutakula kwe-DNA fragment kungaqedwa kungakapheli imizuzu eyi-15 futhi i-primer dimer ingasuswa ngokuqondile ngaphandle kokutholwa kwejeli.
◮ Safety:Akukho kukhishwa kwe-organic reagent edingekayo.
◮ Ikhwalithi ephezulu: Izingcezu ze-DNA ezitholiwe zimsulwa kakhulu, ezingahlangabezana nokuhlolwa okuhlukahlukene okulandelayo.
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Ikhithi Yokuhlukanisa I-FFPE DNA Ikhithi Yokuhlanjululwa Kwe-DNA evela kumasampuli e-FFPE
Hlanza ngokushesha i-genomic DNA yeqophelo eliphezulu kusukela kumathishu ashumekwe upharafini njengezingxenye zamapharafini namabhulokhi epharafini.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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