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Ikhithi Yokuhlukanisa Yezitshalo Isamba se-RNA

Incazelo yekhithi:

I-RNase-Free

Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column

Khipha i-DNA ngaphandle kokwengeza i-DNase

Kulula—yonke imisebenzi iqedwa ezingeni lokushisa legumbi

Ukusheshisa—ukusebenza kungaqedwa ngemizuzu engu-30

Iphephile—ayikho i-organic reagent esetshenzisiwe

foregene strength


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula ethuthukiswe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezahlukahlukene ezinokuqukethwe okuphansi kwama-polysaccharides nama-polyphenols.Kumasampula ezitshalo anama-polysaccharides aphezulu noma okuqukethwe kwama-polyphenols, kunconywa ukusebenzisa i-Plant Total RNA Isolation Plus Kit ukuze uthole imiphumela engcono yokukhipha i-RNA.Ikhithi inikeza ikholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Buffer PSL1, Buffer PS, Buffer PSL2

Isilondolozi PRW1, Isivimbeli PRW2

I-ddH yamahhala ye-RNase2O, Ikholomu Yokuhlanza I-DNA

Ikholomu Ye-RNA Kuphela

Iziyalezo

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-genomic DNA ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngempumelelo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

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Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Ukugeleza komsebenzi

plant total RNA-simple workflow

Umdwebo

Plant Total RNA Isolation Kit6

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, kanye ne-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalananda

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) endaweni eyomile, kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer PSL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).


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