I-Foreasy M-MLV Reverse Transcriptase
Incazelo yekhithi:
I-Foreasy M-MLV Reverse Transcriptase iyireverse transcriptase evezwa kubhaktheriya enziwe nge-E. coli kusetshenziswa ubuchwepheshe bokuhlanganisa kabusha izakhi zofuzo.Kuyi-recombinant DNA polymerase ehlanganisa umucu we-DNA ohambisanayo kusukela ku-RNA enomucu owodwa, i-DNA, noma i-RNA:ingxube ye-DNA.Ayinawo umsebenzi we-RNase H, ukuzinza okuqinile, ukuhlobana okuqinile kwe-RNA, nokuzwela okuphezulu kokutholwa.
Imininingwane
I-Foreasy M-MLV Reverse Transcriptase iyireverse transcriptase evezwa kubhaktheriya enziwe nge-E. coli kusetshenziswa ubuchwepheshe bokuhlanganisa kabusha izakhi zofuzo.Kuyi-recombinant DNA polymerase ehlanganisa umucu we-DNA ohambisanayo kusukela ku-RNA enomucu owodwa, i-DNA, noma i-RNA:ingxube ye-DNA.Ayinawo umsebenzi we-RNase H, ukuzinza okuqinile, ukuhlobana okuqinile kwe-RNA, nokuzwela okuphezulu kokutholwa.
Izingxenye zekhithi
| Isakhi | I-IM-02011 | I-IM-02012 | I-IM-02013 |
| I-Foreasy Reverse Transcriptase (200 U/μL) | 200 KU (1 mL) | 2000 KU (10 mL) | 5000 KU (25 mL) |
| 5 × Kulula I-RT Buffer | 1 mL × 5 | 10 mL × 5 | 10 mL × 12 |
Izici&izinzuzo
-Awukho umsebenzi we-RNase H
-Ukuzinza okuqinile
-Ukuhlangana okuqinile kwe-RNA
-Ukuzwela okuphezulu
Isicelo sekhithi
Ukuhlanganiswa komucu wokuqala we-cDNA ye-RT-PCR kanye ne-RT-PCR yesikhathi sangempela
I-cDNA synthesis ye-cloning nenkulumo.
Isizukulwane se-cDNA probes yama-microarrays anelebuli.
Ukuhlaziywa kwe-Primer extension RNA
Isitoreji kanye Nempilo yeshelufu
Gcina ku -20±5℃ iminyaka emi-2 noma ku--80℃ ukuze uthole isitoreji eside.
