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I-Foreasy M-MLV Reverse Transcriptase

Incazelo yekhithi:

I-Foreasy M-MLV Reverse Transcriptase iyireverse transcriptase evezwa kubhaktheriya enziwe nge-E. coli kusetshenziswa ubuchwepheshe bokuhlanganisa kabusha izakhi zofuzo.Kuyi-recombinant DNA polymerase ehlanganisa umucu we-DNA ohambisanayo kusukela ku-RNA enomucu owodwa, i-DNA, noma i-RNA:ingxube ye-DNA.Ayinawo umsebenzi we-RNase H, ukuzinza okuqinile, ukuhlobana okuqinile kwe-RNA, nokuzwela okuphezulu kokutholwa.

foregene strength


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane

I-Foreasy M-MLV Reverse Transcriptase iyireverse transcriptase evezwa kubhaktheriya enziwe nge-E. coli kusetshenziswa ubuchwepheshe bokuhlanganisa kabusha izakhi zofuzo.Kuyi-recombinant DNA polymerase ehlanganisa umucu we-DNA ohambisanayo kusukela ku-RNA enomucu owodwa, i-DNA, noma i-RNA:ingxube ye-DNA.Ayinawo umsebenzi we-RNase H, ukuzinza okuqinile, ukuhlobana okuqinile kwe-RNA, nokuzwela okuphezulu kokutholwa.

Izingxenye zekhithi

Isakhi

I-IM-02011

I-IM-02012

I-IM-02013

I-Foreasy Reverse Transcriptase

(200 U/μL)

200 KU (1 mL)

2000 KU (10 mL)

5000 KU (25 mL)

5× Kulula

I-RT Buffer

1 mL × 5

10 mL × 5

10 mL × 12

 

Izici&izinzuzo

-Awukho umsebenzi we-RNase H

-Ukuzinza okuqinile

-Ukuhlangana okuqinile kwe-RNA

-Ukuzwela okuphezulu

 

Isicelo sekhithi

Ukuhlanganiswa komucu wokuqala we-cDNA ye-RT-PCR kanye ne-RT-PCR yesikhathi sangempela

I-cDNA synthesis ye-cloning nenkulumo.

Isizukulwane se-cDNA probes yama-microarrays anelebuli.

Ukuhlaziywa kwe-Primer extension RNA

Isitoreji kanye Nempilo yeshelufu

Gcina kokuthi -20±5℃ iminyaka emi-2 noma ku--80℃ ukuze uthole isitoreji sesikhathi eside.


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