• facebook
  • linkedin
  • youtube
English
isibhengezo
  • I-RT-qPCR Easyᵀᴹ (Isinyathelo esisodwa)-SYBR Green I

    I-RT-qPCR Easyᵀᴹ (Isinyathelo esisodwa)-SYBR Green I

    ◮Ikhithi yesinyathelo esisodwa yenza ukuloba okuhlanekezelwe kanye ne-qPCR ukusabela okubili kushubhu efanayo, idinga kuphela ukwengeza ithempulethi i-RNA, iziqalo ezithile ze-PCR kanye ne-RNase-Free ddH.2O.

    ◮Ikhithi ingahlaziya ngokushesha nangempumelelo i-RNA yegciwane noma ilandele i-RNA.

    ◮Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.

    ◮Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela kokutholwa okuphezulu, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.

    ◮I-RT-qPCR EasyTM(One Step) -SYBR Green I kit iza nodayi wereferensi wangaphakathi we-ROX, ongasetshenziswa ukuqeda ingemuva lesignali namaphutha esignali phakathi kwemithombo, okulula ukuthi amakhasimende ayisebenzise kumamodeli ahlukene wamathuluzi e-PCR amaningi.

    amandla angaphambili

    uphenyoimininingwane
  • I-RT-qPCR Easyᵀᴹ (Isinyathelo Esisodwa)-Taqman

    I-RT-qPCR Easyᵀᴹ (Isinyathelo Esisodwa)-Taqman

    - One-Ikhithi yesinyathelo inika amandla ukulotshwa okuhlanekezelwe kanye ne-PCR ukuthi kwenziwe ngeshubhu efanayo, kudingeka kuphela ukwengeza isifanekiso se-RNA, iziqalo ezithile ze-PCR kanye ne-RNase-Free ddH2O.

    - Ukuhlaziywa kobuningi besikhathi sangempela kwe-RNA yegciwane noma ukulandelela i-RNA kungenziwa ngokushesha nangokunembile.

    - Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.

    - Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela okuphezulu kokutholwa, ukuqina okushisayo okuqinile, nokubekezelelana okungcono.

    - RT-qPCR EasyTM(One Isinyathelo) -Ikhithi ye-Taqman iza nodayi wereferensi wangaphakathi we-ROX, ongasetshenziswa ukuqeda ingemuva lesignali namaphutha esignali phakathi kwemithombo, elungele abasebenzisi bokugcina ukuyisebenzisa kumamodeli ahlukene wamathuluzi e-PCR amaningi.

    amandla angaphambili

    uphenyoimininingwane
Chofoza u-enter ukuze useshe noma u-ESC ukuze uvale