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Ikhithi yesitshalo eqondile ye-PCR Plus (ngaphandle kwamathuluzi okusampula) I-Protocol Direct PCR evela ku-Plant Material
Sebenzisa ngokuqondile i-polysaccharide ne-polyphenol leaf lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.
◮Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)
◮Impahla encane
◮Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
◮2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela
◮Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50
◮Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96
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Iqabunga lesitshalo I-PCR eqondile kanye nekhit-UNG (ngaphandle kwamathuluzi okusampula) Iphrothokholi eqondile ye-PCR evela ku-Plant Material
Sebenzisa ngokuqondile i-polysaccharide ne-polyphenol leaf lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.
◮Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)
◮Impahla encane
◮Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
◮2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela
◮Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50
◮Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96
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Iqabunga lesitshalo Direct PCR kit-UNG (ngaphandle kwamathuluzi okusampula) Iphrothokholi eqondile ye-PCR evela ku-Plant Material
Sebenzisa ngokuqondile iqabunga noma i-lysate yeqabunga njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.
◮Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)
◮Impahla encane
◮Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
◮2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela
◮Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50
◮Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96
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Ikhithi ye-PCR eqondile yeqabunga (ngaphandle kwamathuluzi okusampula) Iphrothokholi eqondile ye-PCR evela ku-Plant Material
Sebenzisa ngokuqondile iqabunga noma i-lysate yeqabunga njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.
◮Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)
◮Impahla encane
◮Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
◮2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela
◮Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50
◮Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96
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