Hlanza ngokushesha i-plasmid DNA yekhwalithi ephezulu kubhaktheriya eguquliwe ukuze uthole ukuhlolwa okujwayelekile kwebhayoloji yamangqamuzana njengokuguqulwa nokugaya kwe-enzyme.
◮Susa i-RNA ngaphandle kokwengeza i-RNase
◮Elula-i-centrifugation yenziwa ekamelweni lokushisa
◮Ngokushesha-ukuhlinzwa kungaqedwa ngemizuzu engama-20
◮Kuphephile-akukho reagent ephilayo esetshenzisiwe
◮Ukuhlanzeka okuphezulu—OD260/280≈1.7-1.9