◮Ukuze uthole i-RNA eqondile yokukhulisa isikhathi sangempela kusuka kumaqoqo we-swab ngaphandle kwezinqubo zokuhlanza i-RNA.Le khithi iqeda imijikelezo ye-qRT-PCR phakathi nehora elilodwa.Ingxube ye-enzyme iyinhlanganisela elungiselelwe ye-Reverse Transcriptase, i-Hot-Start Taq DNA polymerase, i-RNase inhibitor.I-Reaction Buffer iqukethe zonke izingxenye ezidingekayo, okuhlanganisa izingxenye zebhafa ezithuthukisiwe, uMg2+, i-dUTP, nama-dNTP.