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I-RT-PCR Easyᵀᴹ I(Isinyathelo esisodwa)
◮Ikhithi yesinyathelo esisodwa yenza ukuba ukuloba okuhlanekezelwe kanye ne-PCR kwenziwe ngeshubhu efanayo.Idinga kuphela ukwengeza isifanekiso se-RNA, iziqalisi ze-PCR ezithile kanye ne-ddH Yamahhala ye-RNase2O.
◮Ukuhlaziywa komthamo wesikhathi sangempela se-RNA kungenziwa ngokushesha nangokunembile.
◮Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
◮Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela kokutholwa okuphezulu, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.
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