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I-RT-PCR Easyᵀᴹ I(Isinyathelo esisodwa)
◮Ikhithi yesinyathelo esisodwa yenza ukuba ukuloba okuhlanekezelwe kanye ne-PCR kwenziwe ngeshubhu efanayo.Idinga kuphela ukwengeza isifanekiso se-RNA, iziqalisi ezithile ze-PCR kanye ne-RNase-Free ddH2O.
◮Ukuhlaziywa kobuningi besikhathi sangempela se-RNA kungenziwa ngokushesha nakancane.
◮Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukukhulisa amandla kanye nokucaciswa kokusabela.
◮Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela okuphezulu kokutholwa, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.
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