I-RNAlater (Yokuqiniswa kwe-RNA) Isixazululo Sokuqinisa I-RNAlater
Incazelo yekhithi:
Ukulungiswa okusheshayo kwezicubu zesampula yezilwane ezisanda kuthathwa, kuvimbela umsebenzi we-RNase, kuvikela i-RNA ekonakalisweni.
-Ama-reagents asetshenziswa ngokuqondile, isinyathelo esisodwa sisendaweni, futhi i-RNA iqiniswa ngokushesha futhi ivikelwe.
-Ukusebenza ekamelweni lokushisa, kulula, kuphephile futhi akunabuthi.
-Ukuqeda inkathazo yeqhwa elomile noma isiqandisi esinezinga lokushisa eliphansi lokulondoloza izicubu.
-Izicubu zingagcinwa isikhathi eside ngaphandle kwengozi yokuwohloka kwe-RNA, kuqinisekiswe idatha ethembekile yephrofayili yokubonakaliswa kofuzo.
Izincazelo
I-RNAlater iyi-reagent egcina izicubu zesilwane eziwuketshezi ezingenabuthi.Ingangena ngokushesha kumangqamuzana ezicubu futhi ivikele i-RNA yeselula engaqandisiwe ekuwohlokeni ngokuvimbela ngempumelelo umsebenzi we-RNase, ukuze ngemva kokutholwa kwesampula yethishu, akudingekile ukucubungula isampula ngokushesha, futhi akudingekile ukufriza isampula ku-nitrogen ewuketshezi.,ngakhoilungele kakhulu imisebenzi yokuhlola elandelayo.Ukusetshenziswa kwesisombululo sokuvikela izicubu ze-RNA kungagwema ukuphazamiseka kokusebenzisa i-nitrogen ewuketshezi noma iziqandisi zokushisa eziphansi kakhulu, futhi ukugcina amaqoqo ahlukene ezibonelo zethishu kusixazululo sokuvikela kungamisa ngokushesha futhi kulungise izinguquko zokulandelana kwesisho se-RNA, okunganciphisa iphutha phakathi kwamaqembu okuhlola .
I-RNAlater ingasetshenziswa kabanzi kumasampula ezilwane ezinomgogodla ahlukahlukene, okuhlanganisa ubuchopho, inhliziyo, izinso, ubende, isibindi, namaphaphu.Ngemva kokuba izicubu ezintsha ezingaqandisiwe zicwiliswe ku-Buffer RNAlater ngesilinganiso esingu-1:10, amasampula angagcinwa endaweni yokushisa yasekamelweni isonto eli-1, 37°C usuku olu-1, 4°C okungenani inyanga engu-1;izicubu zingacwiliswa ku-4°C ku -20°C nomasidabukile ku -80 ℃ isikhathi eside.
Imininingwane
50 ml, 100 ml, 100*4 ml
Izingxenye zekhithi
| I-RNAkamuvaOkokuqiniswa kwe-RNA | |||
| Ikati.No. | I-RL-01011 | I-RL-01012 | I-RL-01013 |
| Isilondolozi se-RNAkamuva | 50ml | 100ml | 100ml×4 |
| Incwadi Yeziqondiso | 1 ucezu | 1 ucezu | 1 ucezu |
Izici&izinzuzo
-Ama-reagents asetshenziswa ngokuqondile, isinyathelo esisodwa sisendaweni, futhi i-RNA iqiniswa ngokushesha futhi ivikelwe.
-Ukusebenza ekamelweni lokushisa, kulula, kuphephile futhi akunabuthi.
-Ukuqeda inkathazo yeqhwa elomile noma isiqandisi esinezinga lokushisa eliphansi lokulondoloza izicubu.
-Izicubu zingagcinwa isikhathi eside ngaphandle kwengozi yokuwohloka kwe-RNA, kuqinisekiswe idatha ethembekile yephrofayili yokubonakaliswa kofuzo.
Imingcele yekhithi
I-RNAkamuva Isicelo
Isetshenziselwa ukulondoloza ngokushesha izicubu zezilwane ezisanda kuqoqwa futhi kumiswe ngokushesha i-RNA kumasampula ezicubu ukuze kulondolozwe amaphrofayili wokuvezwa kofuzo.
Izimo zokugcina
Ingagcinwa ngokuzinza isikhathi esingaphezu konyaka ongu-1 ekamelweni lokushisa (15-25℃).
