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Ikhithi yokuhlukanisa i-RNA yezilwane
Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.Lonke uhlelo alunayo i-RNase-Free
Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
Khipha i-DNA ngaphandle kokwengeza i-DNase
Kulula—yonke imisebenzi iqedwa ezingeni lokushisa legumbi
Ukusheshisa—ukusebenza kungaqedwa ngemizuzu engu-30
Iphephile—ayikho i-organic reagent esetshenzisiwe
Ukuhlanzeka okuphezulu—OD260/280≈1.8-2.1
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Ikhithi Yokuzihlukanisa Yeseli Ye-RNA
I-RNase-Free
Isebenza ekamelweni lokushisa (15-25 ℃)
Ngekholomu Yokuhlanza I-DNA
Inani eliphakeme kakhulu lama-RNA
Ukushesha:Qeda ukukhipha emizuzwini eyi-11
Ukuphepha:Ayikho i-Organic chemical
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I-Plant Total RNA Isolation Kit Plus
I-RNase-Free Ukusebenzisa Ikholomu Yokuhlanza I-DNA Ilula—yonke imisebenzi iqedwa ekamelweni lokushisa Ngokushesha—ukusebenza kungaqedwa emizuzwini engu-30 Kuphephile—akukho mshini wezinto eziphilayo osetshenziswayo.
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I-Animal miRNA Isolation Kit
◆ Yonke le nqubo iqhutshwa ekamelweni lokushisa (15-25°C), ngaphandle kokugeza kweqhwa kanye ne-centrifugation enezinga lokushisa eliphansi.
◆Isethi egcwele yekhithi i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
◆Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
◆Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngempumelelo.
◆Isivinini esisheshayo: Ukusebenza kulula futhi kungaqedwa phakathi nemizuzu engama-30.
◆Ukuphepha: akukho kukhishwa kwe-organic reagent edingekayo.
◆Ikhwalithi ephezulu: Izingcezu ze-RNA ezikhishiwe zinokuhlanzeka okuphezulu, azinawo amaprotheni nokunye ukungcola, futhi zingahlangabezana nezidingo zomfula ongezansi.
◆Izinhlelo zokusebenza zokuhlola ezihlukahlukene
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Ikhithi Yokuhlukanisa Yezitshalo Isamba se-RNA
I-RNase-Free
Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
Khipha i-DNA ngaphandle kokwengeza i-DNase
Kulula—yonke imisebenzi iqedwa ezingeni lokushisa legumbi
Ukusheshisa—ukusebenza kungaqedwa ngemizuzu engu-30
Iphephile—ayikho i-organic reagent esetshenzisiwe
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Ikhithi yokuhlukanisa i-Viral DNA&RNA
Akukho ukucekelwa phansi kwe-RNA.Yonke ikhithi i-RNase-Free
Kulula—yonke imisebenzi iqedwa ezingeni lokushisa legumbi
Ukusheshisa—ukusebenza kungaqedwa ngemizuzu engu-20
Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngempumelelo
Iphephile—ayikho i-organic reagent esetshenzisiwe
Umthamo omkhulu wokucubungula isampula—amasampula angafika ku-200μl angacutshungulwa isikhathi ngasinye.
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I-RNAlater (Yokuqiniswa kwe-RNA)
-Ama-reagents asetshenziswa ngokuqondile, isinyathelo esisodwa sisendaweni, futhi i-RNA iqiniswa ngokushesha futhi ivikelwe.
-Ukusebenza ekamelweni lokushisa, kulula, kuphephile futhi akunabuthi.
-Ukuqeda inkathazo yeqhwa elomile noma isiqandisi esinezinga lokushisa eliphansi lokulondoloza izicubu.
-Izicubu zingagcinwa isikhathi eside ngaphandle kwengozi yokuwohloka kwe-RNA, kuqinisekiswe idatha ethembekile yephrofayili yokubonakaliswa kofuzo.
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Ikhithi yokuhlukanisa i-Viral RNA
-Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.Yonke ikhithi i-RNase-Free
-Kulula—yonke imisebenzi iqedwa ezingeni lokushisa elilingana negumbi
-Fast-ukusebenza kungaqedwa ngemizuzu engama-20
-Isivuno esiphezulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo
-Kuphephile—akukho mshini wokwenza izinto eziphilayo osetshenzisiwe
-Umthamo omkhulu wokucubungula isampula - amasampula angafika ku-200μl angacutshungulwa isikhathi ngasinye.
-Ikhwalithi ephezulu—i-RNA ecwengiwe imsulwa kakhulu, ayinaprotheyini nokunye ukungcola, futhi ingahlangabezana nezinhlelo zokusebenza zokuhlola ezehla nomfula.
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I-imeyili
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