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I-RT-PCR ubuchwepheshe obuhlanganisa ukubhalwa kabusha kwe-RT ye-RNA kanye ne-polymerase chain amplification (PCR) ye-cDNA.Ubuchwepheshe be-RT-PCR buyazwela futhi buguquguquka, futhi bungasetshenziswa ukuthola izinga lokuvezwa kofuzo kumaseli/izicubu, okuqukethwe kwamagciwane e-RNA kumaseli, kanye nokuhlanganisa okuqondile kokulandelana kwe-cDNA kwezakhi zofuzo ezithile.

UBUHLE OMUNTU:

✱ Ikhithi ye-RT ye-Foregene inokuqina okuqinile ku-50-65℃ lokushisa lokusabela (65°C ingavula ileveli yesibili ye-RNA ukuze yenze ukusabela kokuhlehla kokulotshiweyo kuqhubeke kahle)

✱ Ukubekezelela okuqinile, kungabekezelela i-ethanol ethile kanye nosawoti we-guanidine

I-sub-Uchungechunge

Igama Lomkhiqizo

Imininingwane

Inombolo Yekhathalogi

Izimo Zokugcina

PCR Mix Series

PCR IqhaweTM

5ml

PH-01013

-20 ℃

40ml

PH-01015

PCR IqhaweTM(Ngodayi)

5ml

PH-01013-B

-20 ℃

40ml

PH-01015-B

I-2×Isistimu ye-PCR yokusabela esebenza kahle kakhulu
I-PCR elulaTM

1ml

DP-20031

-20 ℃

3×1.7ml

DP-20032

I-PCR elulaTM(ngodayi

3×1.7ml

DP-20032-B

-20 ℃

50ml

DP-20034-B

2×Rapid PCR reaction premix system 2× PCR Mix

1ml

DP-20041

-20 ℃

3×1.7ml

DP-20042

Isikhathi Sangempela PCR EasyTM-SYBR Green I

500 × 20μl rxns

QP-01012

-20 ℃

2000 × 20μl rxns

QP-01014

Isikhathi Sangempela PCR EasyTMTaqman

500 × 20μl rxns

QP-01022

-20 ℃

2000 × 20μl rxns

QP-01024

Uchungechunge lwe-RT

I-RT EasyTM I

(I-Master Premix ye-first-strand cDNA synthesis)

25×20μl Ukusabela

I-RT-01011

-20 ℃

100 × 20μl Ukusabela

I-RT-01012

I-RT EasyTM II

(I-Master Premix ye-first-strand cDNA synthesis ye-Real Time PCR)

200 × 10μl Ukusabela

I-RT-01022

-20 ℃

800 × 10μl Ukusabela

RT-01023

I-RT EasyTM II(Nge-gDNase)

I-Master Premix ye-first-strand cDNA synthesis ye-Real Time PCR ene-gDNase

100 × 20μl Ukusabela

I-RT-01032

-20 ℃

Lnc-RT IqhaweTM I(With gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA)

25 × 20μl Ukusabela

I-RTL-09098

-20 ℃

50 × 20μl Ukusabela

I-RTL-09099

I-RT-PCR Series

I-RT-PCR KululaTM I(Isinyathelo esisodwa)

100 rxn

RT-02011

-20 ℃

500 rxn

RT-02012

I-RT-PCR KululaTM II(Izinyathelo ezimbili)

100 rxn

RT-02021

-20 ℃

500 rxn

RT-02022

Uchungechunge lwe-RT-qPCR

I-RT-qPCR EasyTM Mina (Isinyathelo esisodwa)-SYBR Green I

100 rxn

RT-02111

-20 ℃

500 rxn

RT-02112

I-RT-qPCR EasyTM II (Isinyathelo esisodwa)- Taqman

100 rxn

RT-02131

-20 ℃

500 rxn

RT-02132

I-DNA Marker

100bp DNA Ladder

50 Preps

I-DL-20011

4℃

200 Preps

I-DL-20012

1kb DNA Ladder

50 Preps

I-DL-30011

4℃

200 Preps

I-DL-30012