I-RT-PCR ubuchwepheshe obuhlanganisa ukubhalwa kabusha kwe-RT ye-RNA kanye ne-polymerase chain amplification (PCR) ye-cDNA.Ubuchwepheshe be-RT-PCR buyazwela futhi buguquguquka, futhi bungasetshenziswa ukuthola izinga lokuvezwa kofuzo kumaseli/izicubu, okuqukethwe kwamagciwane e-RNA kumaseli, kanye nokuhlanganisa okuqondile kokulandelana kwe-cDNA kwezakhi zofuzo ezithile.
UBUHLE OMUNTU:
✱ Ikhithi ye-RT ye-Foregene inokuqina okuqinile ku-50-65℃ lokushisa lokusabela (65°C ingavula ileveli yesibili ye-RNA ukuze yenze ukusabela kokuhlehla kokulotshiweyo kuqhubeke kahle)
✱ Ukubekezelela okuqinile, kungabekezelela i-ethanol ethile kanye nosawoti we-guanidine
I-sub-Uchungechunge | Igama Lomkhiqizo | Imininingwane | Inombolo Yekhathalogi | Izimo Zokugcina |
PCR Mix Series | 5ml | PH-01013 | -20 ℃ | |
40ml | PH-01015 | |||
5ml | PH-01013-B | -20 ℃ | ||
40ml | PH-01015-B | |||
I-2×Isistimu ye-PCR yokusabela esebenza kahle kakhulu | 1ml | DP-20031 | -20 ℃ | |
3×1.7ml | DP-20032 | |||
3×1.7ml | DP-20032-B | -20 ℃ | ||
50ml | DP-20034-B | |||
2×Rapid PCR reaction premix system 2× PCR Mix | 1ml | DP-20041 | -20 ℃ | |
3×1.7ml | DP-20042 | |||
500 × 20μl rxns | QP-01012 | -20 ℃ | ||
2000 × 20μl rxns | QP-01014 | |||
500 × 20μl rxns | QP-01022 | -20 ℃ | ||
2000 × 20μl rxns | QP-01024 | |||
Uchungechunge lwe-RT | 25×20μl Ukusabela | I-RT-01011 | -20 ℃ | |
100 × 20μl Ukusabela | I-RT-01012 | |||
(I-Master Premix ye-first-strand cDNA synthesis ye-Real Time PCR) | 200 × 10μl Ukusabela | I-RT-01022 | -20 ℃ | |
800 × 10μl Ukusabela | RT-01023 | |||
I-Master Premix ye-first-strand cDNA synthesis ye-Real Time PCR ene-gDNase | 100 × 20μl Ukusabela | I-RT-01032 | -20 ℃ | |
Lnc-RT IqhaweTM I(With gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA) | 25 × 20μl Ukusabela | I-RTL-09098 | -20 ℃ | |
50 × 20μl Ukusabela | I-RTL-09099 | |||
I-RT-PCR Series | 100 rxn | RT-02011 | -20 ℃ | |
500 rxn | RT-02012 | |||
100 rxn | RT-02021 | -20 ℃ | ||
500 rxn | RT-02022 | |||
Uchungechunge lwe-RT-qPCR | 100 rxn | RT-02111 | -20 ℃ | |
500 rxn | RT-02112 | |||
100 rxn | RT-02131 | -20 ℃ | ||
500 rxn | RT-02132 | |||
I-DNA Marker | 50 Preps | I-DL-20011 | 4℃ | |
200 Preps | I-DL-20012 | |||
50 Preps | I-DL-30011 | 4℃ | ||
200 Preps | I-DL-30012 |