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Ikhithi ye-ForeDirect RT-qPCR
◮Ukuze uthole i-RNA eqondile yokukhulisa isikhathi sangempela kusuka kumaqoqo we-swab ngaphandle kwezinqubo zokuhlanza i-RNA.Le khithi iqeda imijikelezo ye-qRT-PCR phakathi nehora elilodwa.Ingxube ye-enzyme iyinhlanganisela elungiselelwe ye-Reverse Transcriptase, i-Hot-Start Taq DNA polymerase, i-RNase inhibitor.I-Reaction Buffer iqukethe zonke izingxenye ezidingekayo, okuhlanganisa izingxenye zebhafa ezithuthukisiwe, uMg2+, i-dUTP, nama-dNTP.
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Ikhithi Yokuhlukanisa I-FFPE DNA Ikhithi Yokuhlanjululwa Kwe-DNA evela kumasampuli e-FFPE
Hlanza ngokushesha i-genomic DNA yeqophelo eliphezulu kusukela kumathishu ashumekwe upharafini njengezingxenye zamapharafini namabhulokhi epharafini.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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I-PCR Purification Kit PCR Clean-up System Ye-Ultra-pure DNA
Hlanza ngokushesha futhi uthole izingcezu ze-DNA zekhwalithi ephezulu ohlelweni lwe-PCR;uma ufuna ukuhlukanisa futhi uthole izingcezu ezithile ze-DNA, sicela ukhethe ikhithi yokutakula ijeli.
◮ Uhlu olubanzi lokutholwa kwe-DNA: Izingcezu ze-DNA ezimfishane njengo-60bp futhi ezinkulu njengo-10kb zingatholwa.
◮ Ukusebenza kahle kokutakula: Ukusebenza kahle kokutakula ngokuvamile kungaphezu kwama-80%, futhi okuphezulu kakhulu kungafinyelela ngaphezu kwama-95%.
◮ Isivinini esisheshayo:Kulula ukusebenza, ukutakula kwe-DNA fragment kungaqedwa kungakapheli imizuzu eyi-15 futhi i-primer dimer ingasuswa ngokuqondile ngaphandle kokutholwa kwejeli.
◮ Safety:Akukho kukhishwa kwe-organic reagent edingekayo.
◮ Ikhwalithi ephezulu: Izingcezu ze-DNA ezitholiwe zimsulwa kakhulu, ezingahlangabezana nokuhlolwa okuhlukahlukene okulandelayo.
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Umenzeli Wokukhishwa Kwesampula
◮Kulula ukusebenza:isampula lysis ngenkathi isampula iqoqo
◮Kushesha futhi kulula:Asikho isidingo sokukhishwa kwe-nucleic acid, okufanele ukutholwa ngezinga elikhulu
◮Ububanzi bohlelo lokusebenza:I-PCR ngokuqondile ngemva kokuqoqwa kwesampula
◮Iphephile futhi inokwethenjelwa:Yenza igciwane lingasebenzi ngokushesha, qinisekisa ukuphepha ngezinga elikhulu kakhulu
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I-RT-PCR Easyᵀᴹ I(Isinyathelo esisodwa)
◮Ikhithi yesinyathelo esisodwa yenza ukuba ukuloba okuhlanekezelwe kanye ne-PCR kwenziwe ngeshubhu efanayo.Idinga kuphela ukwengeza isifanekiso se-RNA, iziqalisi ze-PCR ezithile kanye ne-ddH Yamahhala ye-RNase2O.
◮Ukuhlaziywa komthamo wesikhathi sangempela se-RNA kungenziwa ngokushesha nangokunembile.
◮Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
◮Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela kokutholwa okuphezulu, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.
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I-RT-PCR Easyᵀᴹ II(Izinyathelo ezimbili)
◮Ukuhlaziywa komthamo wesikhathi sangempela se-RNA kungenziwa ngokushesha nangokunembile.
◮Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
◮ Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela kokutholwa okuphezulu, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.
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I-Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits from Mouse Tail
Ikhithi eshesha kakhulu emhlabeni yokukhipha i-genomic DNA yomsila wegundane, engakhipha i-DNA yekhwalithi ephezulu emsileni wegundane lingakapheli ihora elingu-1.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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Ikhithi ye-Bacterial DNA Isolation Kit I-Bacterial Genomic DNA Extraction Purification Kits
Hlanza ngokushesha i-DNA ye-genomic yekhwalithi ephezulu kubhaktheriya esigabeni sokukhula se-logarithmic.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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I-CCND1/IGH I-Dual Color Fusion Probe
Ngokomgomo wokumatanisa okuhambisanayo kwesisekelo se-DNA, i-CCND1 i-orange-red probe kanye ne-IGH green probe yasetshenziswa ukuze kuhlanganiswe nokulandelana okuqondiwe kwe-DNA ku-nucleus, futhi ulwazi lwesimo sofuzo ku-nucleus lwabonwa futhi lwahlaziywa ngaphansi kwesibonakhulu se-fluorescence.
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I-RB1/1q21 I-Dual Color Probe
I-Fluorescence in situ hybridization (FISH) isuselwe kumgomo wokumataniswa okuhambisanayo kwezisekelo ze-DNA, kanye nokubonwa kwamasiginali we-hybridization we-DNA probes enelebuli ye-fluorescence nokulandelana kwayo okuqondiwe kwe-DNA ku-nucleus yeseli ngaphansi kwesibonakhulu se-fluorescence.
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I-HER2/CSP17 I-Dual Color Probe
I-Fluorescence in situ hybridization (FISH) isuselwe kumgomo wokumataniswa okuhambisanayo kwezisekelo ze-DNA, kanye nokubonwa kwamasiginali we-hybridization we-DNA probes enelebuli ye-fluorescence nokulandelana kwayo okuqondiwe kwe-DNA ku-nucleus yeseli ngaphansi kwesibonakhulu se-fluorescence.
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I-ETV6 Dual Color Break Apart Probe
Ngokomgomo we-DNA base-complementary pairing, i-ETV6 i-orange-red probe kanye ne-ETV6 eluhlaza okotshani kwasetshenziswa ukuze kuhlanganiswe nokulandelana kwethagethi ye-DNA ku-nucleus, futhi ulwazi lwesimo sofuzo ku-nucleus lwabonwa futhi lwahlaziywa ngaphansi kwesibonakhulu se-fluorescence.
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