I-RT-PCR Easyᵀᴹ II(Izinyathelo ezimbili)
Incazelo yekhithi:
◮Ukuhlaziywa komthamo wesikhathi sangempela se-RNA kungenziwa ngokushesha nangokunembile.
◮Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
◮ Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela kokutholwa okuphezulu, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.
Imininingwane
I-RT-PCR KululaTM II(Izinyathelo ezimbili) iqukethe wonke ama-reagents ezinyathelo ezimbili ze-RT-PCR, ehlanganisa ukusabela kwe-RT kanye nokusabela kwe-PCR kukhithi efanayo, ihlinzeka nge-RT Mix ne-PCR Mix.I-RT Mix eyingqayizivele ingahlanganisa i-cDNA yomucu wokuqala wekhwalithi ephezulu kalula nangempumelelo. 2× I-PCRKululaTM HlanganisaNgaphezu kwalokho ine-amplification ephezulu kanye ne-amplification ethize.Inhlanganisela ye-organic yalokhu kokubili yenza ukutholwa kofuzo oluqondiwe lube bucayi kakhulu.
Izingxenye zekhithi
| I-RT-PCRKululaTM II(Izinyathelo ezimbili) | ||
| Okuqukethwe kwekhithi | I-RT-02021 | I-RT-02022 |
| 100T(20μluhlelo) | 500T(20μluhlelo) | |
| 2× RT KululaTM Hlanganisa | 1ml | 1.7mlx3 |
| 2× I-PCRKululaTM HlanganisaNgaphezu kwalokho | 1ml | 1.7mlx3 |
| I-Primer engahleliwe (50μM) | 200μl | 1ml |
| I-Oligo(dT)18 Primer (50μM) | 200μl | 1ml |
| I-ddH yamahhala ye-RNase2O | 1.7ml | 1.7ml |
| Incwadi Yeziqondiso | 1ucezu | 1ucezu |
Izici&izinzuzo
-Ukuhlaziywa komthamo wesikhathi sangempela se-RNA kungenziwa ngokushesha nangokunembile.
-Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
-Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela kokutholwa okuphezulu, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
1.Izimo zokuhamba
Yonke inqubo yokuthutha kwebhokisi leqhwa elisezingeni eliphansi, ukuqinisekisa ukuthi ikhithi isesimweni esingu-<4 °C.
2. Izimo zokugcina
Ikhithi igcinwa ku -20 ° C.Gcina umkhiqizo esiqandisini esingaguquki sezinga lokushisa elingu-20°C ngokushesha ngemva kokuwuthola.Uma izimo zokugcina zifanelekile,tumkhiqizo wakhe ngeke wehlise noma yikuphi ukusebenza phakathi nesikhathi sokuqinisekisa sonyaka ongu-1.
