I-PCR Purification Kit PCR Clean-up System Ye-Ultra-pure DNA
Incazelo yekhithi:
Hlanza ngokushesha futhi uthole izingcezu ze-DNA zekhwalithi ephezulu ohlelweni lwe-PCR;uma ufuna ukuhlukanisa futhi uthole izingcezu ezithile ze-DNA, sicela ukhethe ikhithi yokutakula ijeli.
◮ Uhlu olubanzi lokutholwa kwe-DNA: Izingcezu ze-DNA ezimfishane njengo-60bp futhi ezinkulu njengo-10kb zingatholwa.
◮ Ukusebenza kahle kokutakula: Ukusebenza kahle kokutakula ngokuvamile kungaphezu kwama-80%, futhi okuphezulu kakhulu kungafinyelela ngaphezu kwama-95%.
◮ Isivinini esisheshayo:Kulula ukusebenza, ukutakula kwe-DNA fragment kungaqedwa kungakapheli imizuzu eyi-15 futhi i-primer dimer ingasuswa ngokuqondile ngaphandle kokutholwa kwejeli.
◮ Safety:Akukho kukhishwa kwe-organic reagent edingekayo.
◮ Ikhwalithi ephezulu: Izingcezu ze-DNA ezitholiwe zimsulwa kakhulu, ezingahlangabezana nokuhlolwa okuhlukahlukene okulandelayo.
Incazelo
Le khithi isebenzisa ikholomu yokuphonswa kanye nefomula eyakhiwe inkampani yethu, ekwazi ukubuyisela kahle izingcezwana ze-DNA ezihlanzeke kakhulu ohlelweni lwe-PCR.Ikholomu yokuhlanza ingabopha kahle i-DNA, futhi ifaniswe ne-reagent yefomula eyingqayizivele ukuze isuse ngokuqondile i-primer dimer ngaphandle kokuthola ijeli.
Ikhithi ithola inqwaba yezingcezu ze-DNA.Ngaphansi kwezimo ezijwayelekile, izingcezu ze-DNA ezimfishane njengo-60bp zingatholwa.Ubuncane obungama-30μl we-eluent bungasetshenziswa ukwandisa ukugxila kwe-DNA etholiwe.
Ikhithi kulula ukuyisebenzisa futhi inezinyathelo ezimbalwa.Idinga kuphela i-centrifugation embalwa ukuze icubungule amasampula amaningi ngesikhathi esisodwa, futhi izingcezu ze-DNA ezitholwe ngobumsulwa obuphezulu zingatholwa emizuzwini eyi-15.
Imininingwane
50 Preps, 100Preps, 250Preps
Izingxenye zekhithi
| I-Buffer BD* |
| Isilondolozi BD-S |
| Isilondolozi se-WB1 |
| Isilondolozi EB |
| Ikholomu Ye-DNA Kuphela |
| Iziyalezo |
Izici&izinzuzo
-Uhlu olubanzi lokutholwa kwe-DNA: Izingcezu ze-DNA ezimfishane njengo-60bp futhi ezinkulu njengo-10kb zingatholwa.
-Ukuphumelela okuphezulu kokululama: Ukusebenza kahle kokululama kuvame ukudlula ama-80%, kanti okuphezulu kakhulu kungafinyelela ngaphezu kwama-95%.
-Isivinini esisheshayo: Kulula ukusebenza, ukutakula kwe-DNA fragment kungaqedwa kungakapheli imizuzu eyi-15 futhi i-primer dimer ingasuswa ngokuqondile ngaphandle kokutholwa kwejeli.
-Ukuphepha: Akukho kukhishwa kwe-organic reagent edingekayo.
-Ikhwalithi ephezulu: Izingcezwana ze-DNA ezitholiwe zihlanzekile kakhulu, ezingahlangabezana nokuhlolwa okuhlukahlukene okulandelayo.
Isicelo sekhithi
Le khithi ilungele ukubuyisela izingcezwana ze-DNA (60bp-10kb) emikhiqizweni ye-PCR.
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.
Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.
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