I-RT-PCR Easyᵀᴹ I(Isinyathelo esisodwa)
Incazelo yekhithi:
◮Ikhithi yesinyathelo esisodwa yenza ukuba ukuloba okuhlanekezelwe kanye ne-PCR kwenziwe ngeshubhu efanayo.Idinga kuphela ukwengeza i-RNA yesifanekiso, ama-primers e-PCR athile kanye ne-RNase-Free ddH2O.
◮Ukuhlaziywa komthamo wesikhathi sangempela se-RNA kungenziwa ngokushesha nangokunembile.
◮Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
◮Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela kokutholwa okuphezulu, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.
Imininingwane
I-Foregene One-Step RT-PCR EasyTM imikhiqizo yochungechunge ibona ukusabela okuhlanganisiwe kusuka ku-RNA kuya ku-DNA enemicu ephindwe kabili, okungukuthi, ukuloba okuhlanekezelwe kanye nokukhulisa i-PCR kuqedwa ngeshubhu le-centrifuge lokusabela elifanayo kanye nesistimu yokusabela efanayo, eyenza izinyathelo zokuhlola zibe lula., ilungiselelatyena uhlelo lokuhlola, futhikuyathuthuka ukuhlolwa ukusebenza kahle.
I-RT-PCR KululaTM I(Isinyathelo esisodwa) iyisinyathelo esisodwa se-RT-PCR kit eyenziwe ngokukhethekile yi-Foregene, eqaphela ukusebenza okuqhubekayo ukusuka ku-RT kuya ku-PCR kushubhu efanayo.Ukusebenza kulula futhi kuyashesha, okunganciphisa ngempumelelo ukungcola ngesikhathi sokuhlolwa.2×I-RT-PCR KululaTM Imiksi inokubekezelela okuqinile, ukuzinza kwe-thermal, ukusebenza kahle okuphezulu nokuqondileity for ukukhuliswa.
Izingxenye zekhithi
| I-RT-PCR KululaTMI(Isinyathelo esisodwa) | ||
| Okuqukethwe kwekhithi | RT-02011 | RT-02012 |
| 100T (20μl uhlelo) | 500T (20μl uhlelo) | |
| 2× RT-PCR EasyTM Hlanganisa | 1ml | 1.7ml×3 |
| I-ddH yamahhala ye-RNase2O | 1.7ml | 1.7ml×3 |
| Incwadi Yeziqondiso | 1 isiqephu | 1 ucezu |
Izici&izinzuzo
- Ikhithi yesinyathelo esisodwa yenza ukuloba okuhlanekezelwe kanye ne-PCR kwenziwe ngeshubhu efanayo.Idinga kuphela ukwengeza i-RNA yesifanekiso, ama-primers e-PCR athile kanye ne-RNase-Free ddH2O.
- Ukuhlaziywa kwamanani esikhathi sangempela se-RNA kungenziwa ngokushesha nangokunembile.
- Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
- Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela okuphezulu kokutholwa, ukuqina okushisayo okuqinile, nokubekezelelana okungcono.
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
1.Izimo zokuhamba
Yonke inqubo yokuthutha kwebhokisi leqhwa elisezingeni eliphansi, ukuqinisekisa ukuthi ikhithi isesimweni esingu-<4 °C.
2. Izimo zokugcina
Ikhithi igcinwa ku -20 ° C.Gcina umkhiqizo esiqandisini esingaguquki sezinga lokushisa elingu-20°C ngokushesha ngemva kokuwuthola.Uma izimo zokugcina zifanelekile,tumkhiqizo wakhe ngeke wehlise noma yikuphi ukusebenza phakathi nesikhathi sokuqinisekisa sonyaka ongu-1.
