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1000ul Pipette Amathiphu
◮ 100% i-polypropylene ehlanzekile (PP) impahla eluhlaza
◮ Ukungena kahle, ukufakwa uphawu okushelelayo nokuhle, okuqinisekisa ukunemba kwamapayipi
◮ Izinhlobo eziningi ezitholakalayo
◮ Iguquguquka futhi ijwayelana namapayipi amaningi asekhaya nawamazwe ngamazwe aziwayo emakethe.
◮ Ukuvalwa inzalo nge-gamma irradiation
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1250ul Pipette Amathiphu
◮ 100% i-polypropylene ehlanzekile (PP) impahla eluhlaza
◮ Ukungena kahle, ukufakwa uphawu okushelelayo nokuhle, okuqinisekisa ukunemba kwamapayipi
◮ Izinhlobo eziningi ezitholakalayo
◮ Iguquguquka futhi ijwayelana namapayipi amaningi asekhaya nawamazwe ngamazwe aziwayo emakethe.
◮ Ukuvalwa inzalo nge-gamma irradiation
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10ul Pipette Amathiphu
◮ 100% i-polypropylene ehlanzekile (PP) impahla eluhlaza
◮ Ukungena kahle, ukufakwa uphawu okushelelayo nokuhle, okuqinisekisa ukunemba kwamapayipi
◮ Izinhlobo eziningi ezitholakalayo
◮ Iguquguquka futhi ijwayelana namapayipi amaningi asekhaya nawamazwe ngamazwe aziwayo emakethe.
◮ Ukuvalwa inzalo nge-gamma irradiation
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Ikhithi Yokuhlukanisa I-DNA Yamanzi I-DNA Isizinda kanye Nekhithi Yokuhlanza Yamanzi
Hlanza ngokushesha i-DNA ye-genomic yekhwalithi ephezulu kuma-microorganisms kumasampula amanzi ahlukahlukene.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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Imbewu Yezitshalo (enkulu nephakathi nendawo) I-PCR Plus Kit II-UNG eqondile (ngaphandle kwamathuluzi okusampula)
Sebenzisa ngokuqondile i-polysaccharide polyphenol seed lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
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Imbewu Yezitshalo(Polysaccharide Polyphenol rich Small Small) Direct PCR Plus Kit I (ngaphandle kwamathuluzi okusampula)
Sebenzisa ngokuqondile i-polysaccharide polyphenol seed lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
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Imbewu Yezitshalo(i-Polysaccharide Polyphenol ecebile, enkulu futhi enosayizi omaphakathi) I-Direct PCR Plus Kit II-UNG(ngaphandle Kwamathuluzi Okusampula)
Sebenzisa ngokuqondile i-polysaccharide polyphenol seed lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
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Imbewu Yezitshalo(Enkulu) Eqondile ye-PCR Kit II-UNG(ngaphandle kwamathuluzi okusampula)
Ukusetshenziswa okuqondile kwembewu ye-lysate njengesifanekiso sokukhulisa i-PCR, akukho kukhishwa okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
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Imbewu Yezitshalo(Encane Naphakathi) I-PCR Kit I-UNG eqondile (ngaphandle kwamathuluzi okusampula)
Ukusetshenziswa okuqondile kwembewu ye-lysate njengesifanekiso sokukhulisa i-PCR, akukho kukhishwa okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okunamandla kuma-PCR reaction inhibitors.
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Imbewu Yezitshalo(i-Polysaccharide Polyphenol ecebile, encane) I-Direct PCR Plus Kit I-UNG(ngaphandle Kwamathuluzi Okusampula)
Sebenzisa ngokuqondile i-polysaccharide polyphenol seed lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.
- Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo.
- Isidingo sesampula sincane, thatha nje imbewu eyodwa.
- Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukusebenza kulula.
- Uhlelo lwe-PCR oluthuthukisiwe lwenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.
- Uhlelo lwe-PCR olulwa nokungcola 2× Imbewu ye-PCR EasyTM Mix (UNG), engaqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi iqinisekise ukunemba nokunemba kokukhulisa.
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I-Soil DNA Isolation Kit Extraction Kits & Reagents ye-Soil DNA
Hlanza ngokushesha futhi uthole i-genomic DNA yeqophelo eliphezulu kusuka kumasampula amancane aphilayo enhlabathi.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
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Ikhithi ye-ForeDirect RT-qPCR
◮Ukuze uthole i-RNA eqondile yokukhulisa isikhathi sangempela kusuka kumaqoqo we-swab ngaphandle kwezinqubo zokuhlanza i-RNA.Le khithi iqeda imijikelezo ye-qRT-PCR phakathi nehora elilodwa.Ingxube ye-enzyme iyinhlanganisela elungiselelwe ye-Reverse Transcriptase, i-Hot-Start Taq DNA polymerase, i-RNase inhibitor.I-Reaction Buffer iqukethe zonke izingxenye ezidingekayo, okuhlanganisa izingxenye zebhafa ezithuthukisiwe, uMg2+, i-dUTP, nama-dNTP.
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