Ikhithi ye-Bacterial DNA Isolation Kit I-Bacterial Genomic DNA Extraction Purification Kits
Incazelo yekhithi:
Hlanza ngokushesha i-DNA ye-genomic yekhwalithi ephezulu kubhaktheriya esigabeni sokukhula se-logarithmic.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
Incazelo
Le kit inikeza indlela esheshayo nelula yokukhipha i-DNA ye-genomic kumasiko amagciwane (i-Gram-negative ne-Gram-positive) emithonjeni ehlukahlukene;ingacubungula ngaphansi kwe-3ml yamasiko ebhaktheriya esigabeni sokukhula se-logarithmic (1 × 109 amagciwane).Ikhithi ifaniswe ne-Foregene Protease esebenza kahle kakhulu, evumela u-15-50μg wekhwalithi ephezulu ye-genomic DNA ukuthi ikhishwe lingakapheli ihora elingu-1.Ngaphezu kwalokho, ikhithi ingaphinda ikhiphe izinto zofuzo ngaphandle kwe-genome, njenge-plasmids, i-Cosmid, i-BAC, njll.
I-DNA-kuphela kwe-silica gel matrix material esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele yenkampani, engakhangiswa kahle futhi ngokuqondile i-DNA.Umthamo omkhulu we-adsorption we-DNA ngu-80μg.I-buffer nesistimu ye-elution eyingqayizivele ingakhuphula ukukhishwa kwe-RNA, amaprotheni angcolile, ama-ion kanye nezinye izinhlanganisela eziphilayo kumaseli.Ucezu olukhishiwe lwe-DNA ye-genomic sikhulu, ukuhlanzeka okuphezulu, izinga elizinzile futhi elithembekile, futhi usayizi wesiqeshana se-DNA uzinzile cishe ku-23kb.
Imininingwane
50 Preps, 100 Preps, 250 Preps
Izingxenye zekhithi
| Isilondolozi ML1 |
| Isilondolozi se-ML2 |
| Isilondolozi se-PW |
| Isilondolozi se-WB |
| Isilondolozi EB |
| I-Foregene Protease |
| I-Lysozyme |
| Isilondolozi TE |
| Ikholomu Ye-DNA Kuphela |
| Iziyalezo |
Izici&izinzuzo
-Akukho ukungcoliswa kwe-RNase: Asikho isidingo sokwengeza i-RNase ukuze kukhishwe i-RNA ku-DNA ye-genomic, ukugwema ilabhorethri ukuthi ingangcoliswa yi-RNase yangaphandle.
-Isivinini esisheshayo: I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo futhi ingagaya amasampula ebhaktheriya aphukile ngesikhathi esifushane.
-Okulula: I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
-Ukuphepha: akukho kukhishwa kwe-organic reagent edingekayo.
-Ikhwalithi ephezulu: Izingcezu ze-genomic DNA ezikhishiwe zinkulu, azikho i-RNA, azinayo i-RNase, nokuqukethwe kwe-ion okuphansi kakhulu, okungahlangabezana nezidingo zokuhlolwa okuhlukahlukene.
Isicelo sekhithi
Le khithi ifanele ukuhlanza i-DNA ye-genomic yamasampuli alandelayo: Amagciwane e-Gram-negative kanye namagciwane e-Gram-positive esigabeni sokukhula se-logarithmic.
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
-Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.
Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeke ikhambi kukhithi endaweni yokushisa yasekamelweni isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.
-Isixazululo se-Forgene Protease sinefomula eyingqayizivele, esebenzayo uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezingu-3);uma igcinwe ku-4 ° C, umsebenzi wayo nokuzinza kuyoba ngcono, ngakho-ke kunconywa ukuyigcina ku-4 ° C, khumbula ukuthi ungayigcini ku -20 ° C.
-I-Lysozyme iyimpuphu eyomile, esebenzayo uma igcinwe ku-2-8 ° C isikhathi eside (izinyanga ezingu-3).Ukuze uthole isitoreji eside, sicela usigcine ku -20°C.
