Ikhithi Yokuhlukanisa I-FFPE DNA Ikhithi Yokuhlanjululwa Kwe-DNA evela kumasampuli e-FFPE
Incazelo yekhithi:
Hlanza ngokushesha i-genomic DNA yeqophelo eliphezulu kusukela kumathishu ashumekwe upharafini njengezingxenye zamapharafini namabhulokhi epharafini.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
Incazelo
Le khithi inikezelwe ekukhipheni nasekuhlanzeni i-DNA ye-genomic yekhwalithi ephezulu esiculweni esigxilile se-formaldehyde noma izicubu ezishunyekiwe zikapharafini.
I-genomic DNA yesampula eshumekwe upharafini izokwehliswa ngenxa yesikhathi sokugcina noma indawo ezungezile, okwenza kube nzima ukukhipha i-genomic DNA.Le khithi isebenzisa ifomula elungiselelwe eyingqayizivele, elungele ukukhipha i-genomic DNA kumasampuli ashumekwe upharafini anenani eliphezulu lempumelelo yokukhipha.Yonke inqubo ayidingi ukusebenzisa i-phenol enobuthi, i-chloroform noma imvula ye-isopropanol.
Le khithi isebenzisa ikholomu ezungezayo engahlanganisa ngokuqondile i-DNA, i-Foregene Protease Plus entsha sha, kanye nesistimu ye-buffer eyingqayizivele, engakhipha i-DNA yezinga eliphezulu yezicubu ezishunyekiwe zikapharafini phakathi namahora angu-2-5.
Imininingwane
50 Preps, 100 Preps, 250 Preps
Izingxenye zekhithi
| Isilondolozi FL1 |
| Isilondolozi FL2* |
| Isilondolozi PW* |
| Isilondolozi se-WB |
| Isilondolozi EB |
| I-Foregene Protease Plus |
| Ikholomu Ye-DNA Kuphela |
| Iziyalezo |
Izici&izinzuzo
-Akukho ukungcola kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukugwema ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
-Isivinini esisheshayo: I-Foregene Protease Plus inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi namahora angu-2-5.
-Okulula: I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-4°I-C yezinga lokushisa eliphansi le-centrifugation noma imvula ye-ethanol ye-DNA.
-Ukuphepha: akukho kukhishwa kwe-organic reagent edingekayo.
-Ikhwalithi ephezulu: Izingcezu ze-genomic DNA ezikhishiwe zinkulu, azikho i-RNA, azinayo i-RNase, nokuqukethwe kwe-ion okuphansi kakhulu, okungahlangabezana nezidingo zokuhlolwa okuhlukahlukene.
Isicelo sekhithi
Ilungele ukukhishwa nokuhlanzwa kwe-genomic DNA kumasampula agxilile e-formaldehyde kanye namasampula ezicubu ezishunyekiwe zikapharafini.
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
-Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.
Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeke ikhambi kukhithi endaweni yokushisa yasekamelweni isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.
-Isixazululo se-Forgene Protease Plus sinefomula eyingqayizivele esebenzayo uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezi-3);isebenza kakhulu futhi izinzile uma igcinwe ku-4 ° C, ngakho-ke kunconywa ukuyigcina ku-4 ° C, khumbula ukuthi ungayigcini ku -20 ° C.
