I-China Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits Kumkhiqizi Nomhlinzeki Wegundane |I-Foregene

I-Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits from Mouse Tail

I-Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits kusukela ku-Mouse Tail Isithombe Esifakiwe

Incazelo yekhithi:

Ikhithi eshesha kakhulu emhlabeni yokukhipha i-genomic DNA yomsila wegundane, engakhipha i-DNA yekhwalithi ephezulu emsileni wegundane lingakapheli ihora elingu-1.

◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.

◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.

◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.

◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.

◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo

Ngenxa yesakhiwo kanye nezakhiwo zomsila wegundane ngokwayo, ukukhishwa komsila wegundane i-DNA ye-genomic ihlale ikhathaza abacwaningi: enye iwukuthi isikhathi sokucubungula umsila wegundane side futhi i-DNA ye-genomic iyancipha kalula, enye inzima ukuyiphatha futhi ukuhlanzeka kwe-DNA ye-genomic ekhishwe iphansi.

Le khithi isebenzisa Ikholomu Ye-DNA Kuphela engahlanganisa ngokukhethekile i-DNA, i-Foregene Protease entsha sha kanye nesistimu ye-buffer eyingqayizivele, engagaya amasampula omsila wegundane phakathi nemizuzu engu-45, ngaleyo ndlela inciphise ukuwohloka kwe-DNA ye-genomic futhi ifinyeze isikhathi sokucubungula isampula.Ikhithi ingakhipha u-10-20μg wekhwalithi ephezulu, i-high-purity genomic DNA kusuka ku-0.5-1cm umsila wegundane wezingane noma omdala phakathi kuka-60-90min.

I-DNA-kuphela kwe-silica gel matrix material esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele yenkampani, engakhangiswa kahle futhi ngokuqondile i-DNA.Umthamo omkhulu we-adsorption we-DNA ngu-80μg.I-buffer nesistimu ye-elution eyingqayizivele ingakhuphula ukukhishwa kwe-RNA, amaprotheni angcolile, ama-ion kanye nezinye izinhlanganisela eziphilayo kuseli.Ucezu lwe-DNA ekhishiwe likhulu, liphezulu ngobumsulwa, lizinzile futhi linokwethenjelwa ngekhwalithi, futhi usayizi wocezu lwe-DNA uzinzile cishe ku-23kb.

Ikhithi ingase futhi isetshenziselwe ukukhipha ezinye izicubu zegundane ngaphandle komsila wegundane, okuhlanganisa izindlebe zegundane, imisipha yegundane, isibindi segundane nezinye izicubu, ukuze ikhithi eyodwa isetshenziselwe izinjongo eziningi futhi ihlangabezane nezidingo zabasebenzisi zokuhlola okuhlukile.

Imininingwane

50 Preps, 100 Preps, 250 Preps

Izingxenye zekhithi

I-Buffer TL1

I-Buffer TL2*

Isilondolozi PW*

Isilondolozi se-WB

Isilondolozi EB

I-Foregene Protease Plus

Ikholomu Ye-DNA Kuphela

Iziyalezo

Izici&izinzuzo

-Akukho ukungcola kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukugwema ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

-Isivinini esisheshayo: I-Foregene Protease Plus inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi nemizuzu engama-40.

-Kulula: I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.

-Ukuphepha: Akukho kukhishwa kwe-organic reagent edingekayo.

-Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, engahlangabezana nezidingo zokuhlola okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-DNA ye-genomic kusuka emisileni emisha noma efriziwe emikhulu neyegundane (iphinde isetshenziselwe ukuhlanza igundane noma umsipha wegundane kanye ne-DNA yendlebe yegundane).

Ukuhamba komsebenzi

Isitoreji kanye Nempilo yeshelufu

-Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.

Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.

-Isixazululo se-Forgene Protease Plus sinefomula eyingqayizivele, iyasebenza uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezi-3);igcinwe ku-4 ° C, umsebenzi nokuzinza kuzoba ngcono, ngakho-ke kunconywa ukuyigcina ku-4 ° C, khumbula ukuthi ungayigcini ku -20 ° C.

Okwedlule: I-RT Easyᵀᴹ II Master Premix ye-cDNA yomucu wokuqala we-Real Time PCR

Olandelayo: I-RT-PCR Easyᵀᴹ II(Izinyathelo ezimbili)

Bhala umyalezo wakho lapha futhi usithumelele wona