I-China EndoFree Maxi Plasmid Kit (Ikholomu Ye-Spin) Umkhiqizi Nomhlinzeki |I-Foregene

I-EndoFree Maxi Plasmid Kit (Ikholomu ye-Spin)

Incazelo yekhithi:

Yonke inqubo yokukhipha ithatha ihora elingu-1 kuphela, iqinisekisa ukusebenza okulula nokusheshayo.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo

I-EndoFree Maxi Plasmid Kit isebenzisa indlela yokukhangisa ye-silica membrane eyingqayizivele ukuze ibophe ngempumelelo nangokuqondile i-plasmid DNA.Ngokuhlanganisa i-Endotoxin Removal Buffer P4 ekhethekile kanye ne-EndoFree Maxi Filtration Columns, i-endotoxin, amaprotheni nokunye ukungcola kungasuswa ngempumelelo.Yonke inqubo yokukhipha ithatha ihora elingu-1 kuphela, iqinisekisa ukusebenza okulula nokusheshayo.

Inani lamabhaktheriya anconyiwe ngomkhuba ngamunye: Nge-plasmid enekhophi ephezulu, kunconywa ukusebenzisa u-100 ml wemidiya ye-bacterium culture, kanye nesivuno lapho ngokuvamile silinganiselwa ku-500-1500 μg.Nge-plasmid enekhophi ephansi, imidiya yesiko lamagciwane angama-200 ml iyanconywa ukuthi ikhiqize cishe ama-plasmids angama-200-600 μg.

Izingxenye zekhithi

Umkhiqizo Izingxenye	I-D909-E(10 preps)
Isilondolozi BL	30 ml
Isilondolozi se-P1	100 ml
Isilondolozi se-P2	100 ml
Isilondolozi se-P4	100 ml
Isilondolozi se-DW2	44 ml
Isilondolozi EB	30 ml
I-RNase A(10 mg/ml)	1 ml
Amakholomu e-DNAPure Maxi Spin	10 ama-pcs
Isirinji Sesihlungi se-FinePure Maxi	10 ama-pcs
50 ml Amashubhu wokuqoqa	10 ama-pcs

Ukutholwa kwePlasmid DNA Concentration and Purity

Ukuhlanzeka nokugxila kwe-plasmid DNA ekhishiwe kungatholwa nge-agarose gel electrophoresis kanye ne-ultraviolet spectrophotometer.Inani le-OD260 lika-1 lihambisana cishe ne-50 μg/ml ye-DNA enemicu ekabili.Inani le-OD260/OD280 le-plasmid DNA ehlanzekile livamise ukuba kububanzi obungu-1.7-1.9.I-plasmid DNA ehlanzekile ingasetshenziswa ngokuqondile ekuhlolweni okunezidingo zokuhlanzeka okuphezulu, njengokudluliswa kweseli noma ukuhlola kwe-vivo.

Isicelo sekhithi

I-plasmid DNA ekhishwe yile khithi ingasetshenziswa emisebenzini ehlukahlukene yejwayelo, okuhlanganisa ukugaya kwe-enzymatic, i-PCR, ukulandelana, ukuhlanganisa, ukuguqulwa kanye nokudluliswa kwezinhlobo zamaseli ezihlukahlukene.

Isitoreji kanye Nempilo yeshelufu

I-EndoFree Maxi Plasmid Kit ingagcinwa ekamelweni lokushisa (15-25°C) ngaphansi kwezimo ezomile kuze kube yizinyanga eziyi-12.Engeza i-RNase A ku-Buffer P1 ngaphambi kokusetshenziswa kokuqala, hlanganisa kahle futhi ugcine ku-2-8°C.Ingxube ingagcinwa ngokuzinzile isikhathi esingaphezu kwezinyanga eziyisi-6.

Okwedlule: Ikhithi yokukhipha i-DNA/RNA (Ubuhlalu obuzibuthe)

Olandelayo: I-DAPI Counterstain

Bhala umyalezo wakho lapha futhi usithumelele wona