I-General Plasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits
Incazelo yekhithi:
Hlanza ngokushesha i-plasmid DNA yekhwalithi ephezulu kubhaktheriya eguquliwe ukuze uthole ukuhlolwa okujwayelekile kwebhayoloji yamangqamuzana njengokuguqulwa nokugaya kwe-enzyme.
◮Susa i-RNA ngaphandle kokwengeza i-RNase
◮Elula-i-centrifugation yenziwa ekamelweni lokushisa
◮Ngokushesha-ukuhlinzwa kungaqedwa ngemizuzu engama-20
◮Kuphephile-akukho reagent ephilayo esetshenzisiwe
◮Ukuhlanzeka okuphezulu—OD260/280≈1.7-1.9
Izincazelo zekhithi
Imikhiqizo yochungechunge lwe-FOREGENE'S nucleic acid isizinda kanye nokuhlanzwa ingakhishwa futhi ihlanzwe kuma-nucleic acid ekhwalithi ephezulu kusuka emithonjeni ehlukahlukene kanye namasampula ezingxenye eziyinkimbinkimbi.Imikhiqizo yochungechunge lokukhishwa kwe-DNA isekelwe kumakholomu okuhlanza ulwelwesi lwe-silica ye-DNA Kuphela kanye nama-reagents efomula ahlukile angahlanza i-DNA yekhwalithi ephezulu kusukela kumasampuli ahlukahlukene.
I-General Plasmid Mini Kit isebenzisa ubuchwepheshe bekholomu yokuhlanza ye-DNA-Only kanye nefomula ye-SDS lysis esebenzayo ukuze kutholwe i-plasmid DNA yekhwalithi ephezulu kubhaktheriya phakathi nemizuzu engu-20.I-DNA-Kuphela ulwelwesi lwejeli ye-silica esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele ye-FOREGENE, engakhanga i-DNA kahle futhi ngokuqondile, futhi isuse i-RNA, amaprotheni angcolile, ama-ion nezinye izinhlanganisela eziphilayo kumaseli ngezinga elikhulu kakhulu.Umthamo omkhulu wokubopha we-plasmid DNA yekholomu yokuhlanza ye-DNA Kuphela ngu-60μg we-DNA.Inani le-plasmid DNA ekhishwe empeleni lihlobene nomthamo wesisombululo sebhaktheriya, inani lamakhophi e-plasmid (ikhophi ephezulu, ikhophi ephansi), izinkinga, izimo zesiko kanye nezinye izici.
Imininingwane
| I-General Plasmid Mini Kit | |||
| Okuqukethwe kwekhithi | DE-01001 | DE-01002 | DE-01003 |
| 50T | 100T | 250T | |
| Isilondolozi S1 | 10ml | 20ml | 50ml |
| Isivimbeli S2 | 10ml | 20ml | 50ml |
| Isivimbeli S3 | 25ml | 50ml | 125ml |
| Isilondolozi se-PW | 25ml | 50ml | 125ml |
| Isilondolozi se-WB2 | 15ml | 30ml | 75ml |
| Isilondolozi EB | 10ml | 20ml | 50ml |
| Ikholomu Ye-DNA Kuphela | 50 | 100 | 250 |
| Imanuwali | 1 | 1 | 1 |
Izici&izinzuzo
- Akukho ukungcoliswa kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-plasmid DNA ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukugwema ukungcoliswa kwendawo yokucwaninga nge-RNase yangaphandle.
- Isivinini esisheshayo: Ukukhishwa kwe-Plasmid DNA kungaqedwa kungakapheli imizuzu engama-20.
- Kuphephile: Akukho kukhishwa kwe-organic reagent edingekayo.
- Ikhwalithi ephezulu: I-plasmid etholwe ngokukhipha ayinayo i-RNA, ayikho i-RNase kanye nokuqukethwe kwe-ion ephansi kakhulu, engahlangabezana nezidingo zokuhlola okuhlukahlukene.
Isicelo sekhithi
Ukukhishwa kwe-Plasmid DNA kanye nokuhlanzwa kwamabhaktheriya e-Gram-negative.
Ukugeleza komsebenzi
Umdwebo
Isitoreji nempilo yeshelufu
Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25℃);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2–8℃.
Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37℃ imizuzu engu-10 ukuze uncibilikise imvula, futhi uyixube ngaphambi kokuyisebenzisa.
-
I-Animal Tissue DNA Isolation Kit DNA Extraction ...
-
I-EndoFree Maxi Plasmid Kit (Ikholomu ye-Spin)
-
Ikhithi ye-FFPE DNA Isolation Kit DNA Extraction Purifica...
-
Ikhithi ye-Plant DNA Isolation Kit Genomic Plant DNA Purif...
-
I-Blood DNA Midi Kit (1-5ml) I-Blood Isolation Midi...
-
I-Buccal Swab/FTA Card DNA Isolation Kit Genomic ...
