I-China Animal Tissue DNA Isolation Kit I-DNA Extraction Kits from Animal Tissue Samples Umkhiqizi Nomhlinzeki |I-Foregene

Ikhithi Yezicubu Zenyama Yezilwane I-DNA Isolation Kit DNA Extraction Kits from Animal Tissue Samples

Ikhithi Yezicubu Zezilwane I-DNA Isolation Kit DNA Extraction Kits from Animal Tissue Amasampula Isithombe Esifakiwe

Incazelo yekhithi:

Ukukhishwa okusheshayo nokuhlanzwa kwe-genomic DNA emithonjeni eminingi, njengezicubu zezilwane, amaseli, njll.

◮Isivinini esisheshayo:umsebenzi ophezulu kunama-protease afanayo futhi igaye amasampula ezicubu ngokushesha;

◮Kulula:ingaqedwa ngemizuzu engama-50.

◮Kulula:Kwenziwa ezingeni lokushisa legumbi.

◮Ukuphepha:akukho reagent ephilayo esetshenziswayo.

Thumela i-imeyili kithi Landa njenge-PDF

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Izincazelo zekhithi

Le khithi isebenzisa ikholomu ye-DNA kuphela engabopha ngokuqondile i-DNA, i-Foregene protease kanye nesistimu ye-buffer eyingqayizivele.I-DNA yezinga eliphezulu ye-genomic ingakhishwa kumaseli akhulisiwe ahlukahlukene kanye nezicubu zezilwane kungakapheli imizuzu engama-30 kuye kwengama-50.

Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele ye-Foregene, engabopha ngempumelelo nangokukhethekile ku-DNA, futhi ikhulise ukukhishwa kwe-RNA, amaprotheni angcolile, ama-ion nezinye izinhlanganisela eziphilayo kumaseli.I-5-80μg yekhwalithi ephezulu ye-genomic DNA ingahlanzwa kusuka ku-10-50mg izicubu.

Imininingwane

Ikhithi yokuhlukanisa i-DNA yezicubu zezilwane
Okuqukethwe kwekhithi	DE-05011	DE-05012	DE-05013
Okuqukethwe kwekhithi	50T	100T	250T
Isigcinalwazi L1	20ml	40ml	100ml
Ibhafa L2*	20ml	40ml	100ml
Isilondolozi PW*	25ml	50ml	125ml
Isilondolozi se-WB	25ml	50ml	125ml
Isilondolozi EB	10ml	20ml	50ml
I-Foregene Protease	1.25ml	2.5ml	6.5ml
Ikholomu Ye-DNA Kuphela	50	100	250
Imanuwali	1	1	1

*: I-Buffer L2 kanye ne-Buffer PW iqukethe usawoti omuncu ocikayo.Sicela ugqoke amagilavu futhi uthathe izinyathelo zokuzivikela ezifanele lapho usebenza.

Izici&izinzuzo

■ Akukho ukungcoliswa kwe-RNase: Ikholomu Ye-DNA Kuphela enikezwe kukhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kwe-RNase eyengeziwe phakathi nokuhlolwa, ngaleyo ndlela ivimbele ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

■ Isivinini esisheshayo: I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo futhi igaya amasampula ezicubu ngokushesha.

■ Okulula : ukusebenza kwe-genomic DNA extraction kungaqedwa ngemizuzu engama-50.

■ Ilula: I-centrifugation yenziwa ekamelweni lokushisa, akukho 4℃ lokushisa eliphansi centrifugation noma ethanol precipitation of DNA.

■ Ukuphepha: asikho i-reagent ephilayo edingekayo.

■ Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, kungahlangabezana nezidingo zokuhlola okuhlukahlukene.