Ikhithi yokukhipha ijeli ye-DNA Gel Extraction Kit
Incazelo
Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe inkampani yethu, ekwazi ukubuyisela kahle izingcezwana ze-DNA ezihlanzeke kakhulu kujeli ye-agarose.Ikhithi ingabuyisa izingcezu ze-DNA ebangeni elibanzi.Ngaphansi kwezimo ezijwayelekile, izingcezu ze-DNA ze-30bp-10kb zingatholwa.Ubuncane obungama-30μl we-eluent bungasetshenziswa ukwandisa ukugxila kwe-DNA etholiwe.
Ikhithi kulula ukuyisebenzisa futhi inezinyathelo ezimbalwa.Idinga kuphela i-centrifugation embalwa ukuze icubungule amasampula amaningi ngesikhathi esisodwa, futhi izingcezu ze-DNA ezitholwe ngobumsulwa obuphezulu zingatholwa emizuzwini eyi-15.
Imininingwane
50 Preps
I-Buffer GE: Chaza ibhulokhi yejeli ku-60°C ukuze ukhulule izingcezu ze-DNA kujeli.
I-Buffer WB1: Susa ama-ion kasawoti asele ku-DNA.
I-Buffer EB: susa i-DNA kulwelwesi lwekholomu yokuhlanza.
Ikholomu Ye-DNA Kuphela: Ukukhangisa okuqondile kwezingcezu ze-DNA kujeli.
Izingxenye zekhithi
Isivimbeli GE* |
Isilondolozi se-WB1 |
Isilondolozi EB |
Ikholomu Ye-DNA Kuphela |
Iziyalezo |
Izici&izinzuzo
-Uhlu olubanzi lokutholwa kwe-DNA: Izingcezu ze-DNA ezimfishane njengo-30bp futhi ezinkulu njengo-10kb zingatholwa.
-Ukuphumelela okuphezulu kokululama: Ukusebenza kahle kakhulu kokululama kungafinyelela ngaphezu kwama-80%.
-Small uhlelo elution: okungenani 30μl elution isixazululo ingasetshenziswa elution, okungase ngempumelelo ukwandisa lokuhlushwa walulama DNA izingcezwana.
-Isivinini esisheshayo: Kulula ukusebenza, ukutakula kwe-DNA fragment kungaqedwa kungakapheli imizuzu eyi-15.
-Ukuphepha: Akukho kukhishwa kwe-organic reagent edingekayo.
-Ikhwalithi ephezulu: Izingcezwana ze-DNA ezitholiwe zihlanzekile kakhulu, ezingahlangabezana nokuhlolwa okuhlukahlukene okulandelayo.
Isicelo sekhithi
Le khithi ilungele ukubuyisela izingcezu ze-DNA (30bp-10kb) kumajeli e-agarose.
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
Le kit ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25°C), uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.
Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.