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Ikhithi yokukhipha ijeli ye-DNA Gel Extraction Kit

Incazelo yekhithi:

Ngokushesha nangempumelelo thola izingcezu ze-DNA ezingama-20bp-10kb kujeli ye-agarose.

Uhlu olubanzi lokutholwa kwe-DNA:Izingcezu ze-DNA ezimfishane njengo-30bp nezinkulu njengo-10kb zingatholwa.

Ukusebenza kahle kokutakula:Ukuphumelela okuphezulu kakhulu kokululama kungafinyelela ngaphezu kwama-80%.

Ukukhishwa kwesistimu encane:okungenani isixazululo esingu-30μl elution singasetshenziselwa ukuveza, okungase kwandise ngempumelelo ukugcwala kwezingcezu ze-DNA ezitholiwe.

Isivinini esisheshayo:Kulula ukusebenza, ukutakula kwe-DNA fragment kungaqedwa phakathi nemizuzu eyi-15.

Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.

Ikhwalithi ephezulu:Izingcezu ze-DNA ezitholiwe zimsulwa kakhulu, ezingahlangabezana nokuhlolwa okuhlukahlukene okulandelayo.amandla angaphambili


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo

Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe inkampani yethu, ekwazi ukubuyisela kahle izingcezwana ze-DNA ezihlanzeke kakhulu kujeli ye-agarose.Ikhithi ingabuyisa izingcezu ze-DNA ebangeni elibanzi.Ngaphansi kwezimo ezijwayelekile, izingcezu ze-DNA ze-30bp-10kb zingatholwa.Ubuncane obungama-30μl we-eluent bungasetshenziswa ukwandisa ukugxila kwe-DNA etholiwe.

Ikhithi kulula ukuyisebenzisa futhi inezinyathelo ezimbalwa.Idinga kuphela i-centrifugation embalwa ukuze icubungule amasampula amaningi ngesikhathi esisodwa, futhi izingcezu ze-DNA ezitholwe ngobumsulwa obuphezulu zingatholwa emizuzwini eyi-15.

Imininingwane

50 Preps

I-Buffer GE: Chaza ibhulokhi yejeli ku-60°C ukuze ukhulule izingcezu ze-DNA kujeli.

I-Buffer WB1: Susa ama-ion kasawoti asele ku-DNA.

I-Buffer EB: susa i-DNA kulwelwesi lwekholomu yokuhlanza.

Ikholomu Ye-DNA Kuphela: Ukukhangisa okuqondile kwezingcezu ze-DNA kujeli.

Izingxenye zekhithi

Isivimbeli GE*

Isilondolozi se-WB1
Isilondolozi EB
Ikholomu Ye-DNA Kuphela

Iziyalezo

Izici&izinzuzo

-Uhlu olubanzi lokutholwa kwe-DNA: Izingcezu ze-DNA ezimfishane njengo-30bp futhi ezinkulu njengo-10kb zingatholwa.

-Ukuphumelela okuphezulu kokululama: Ukusebenza kahle kakhulu kokululama kungafinyelela ngaphezu kwama-80%.

-Small uhlelo elution: okungenani 30μl elution isixazululo ingasetshenziswa elution, okungase ngempumelelo ukwandisa lokuhlushwa walulama DNA izingcezwana.

-Isivinini esisheshayo: Kulula ukusebenza, ukutakula kwe-DNA fragment kungaqedwa kungakapheli imizuzu eyi-15.

-Ukuphepha: Akukho kukhishwa kwe-organic reagent edingekayo.

-Ikhwalithi ephezulu: Izingcezwana ze-DNA ezitholiwe zihlanzekile kakhulu, ezingahlangabezana nokuhlolwa okuhlukahlukene okulandelayo.

Isicelo sekhithi

Le khithi ilungele ukubuyisela izingcezu ze-DNA (30bp-10kb) kumajeli e-agarose.

Ukuhamba komsebenzi

Ikhithi Yokukhipha Ijeli

Isitoreji kanye Nempilo yeshelufu

Le kit ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25°C), uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.

Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.


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