Ikhithi yokukhipha i-DNA/RNA (Ubuhlalu obuzibuthe)
Incazelo yekhithi:
- Yonke inqubo yokuzihlukanisa iphephile futhi ifanelekile
- I-DNA yamahhala yeseli ekhishiwe inesivuno esikhulu, ukuhlanzeka okuphezulu kanye nekhwalithi ethembekile
Incazelo
Iklanyelwe ukuhlukaniswa okusheshayo nokuthembekile kwekhwalithi ephezulu ye-DNA yamaseli ku-serum ne-plasma.
Ubuchwepheshe obuyingqayizivele bezinhlayiya kazibuthe buhlinzeka ngekhwalithi ephezulu yokukhishwa kwe-DNA ngokushintsha ukuhambisana kwe
kazibutheizinhlayiya.
Iphrothokholi
| Uhlobo | M107 | I-Y107-G50 |
| Kuyasebenzaindlela | Ukusebenza Mathupha | Ukuhlanzwa okuzenzakalelayo(ye-P24) |
Izingxenye zekhithi
| Umkhiqizo Izingxenye | M107 (2ml×50 preps） |
| Isilondolozi se-GHP | 160 ml |
| Isilondolozi se-RBP | 55 ml |
| Amaprotheni K | 12 ml |
| Izinhlayiya ze-FineMag K | 1.8 ml |
| Isilondolozi EB | 15 ml |
Izici&izinzuzo
- Yonke inqubo yokuzihlukanisa iphephile futhi ifanelekile
- I-DNA yamahhala yeseli ekhishiwe inesivuno esikhulu, ukuhlanzeka okuphezulu kanye nekhwalithi ethembekile
Isicelo sekhithi
Ilungele ukuhlukanisa nokuhlanza i-DNA yamahhala yekhwalithi ephezulu ku-plasma.
Isitoreji kanye Nempilo yeshelufu
Wonke ama-Reagents angagcinwa ekamelweni lokushisa (15-25°C) izinyanga ezingu-12.
