Ikhithi Yokuhlukanisa I-DNA Yesitulo I-DNA Extraction Amakhithi Okuhlanza Esitokisini
Incazelo yekhithi:
Hlanza ngokushesha futhi uthole i-DNA ye-genomic yekhwalithi ephezulu kumasampula ezihlalo ahlukahlukene.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
Incazelo
Le kit inikeza indlela esheshayo nelula yokukhipha i-DNA ye-genomic emithonjeni ehlukahlukene yenhlabathi.Kunenani elikhulu lama-inhibitor afana ne-humic acid nama-ion ensimbi kumasampula enhlabathi.Ngisho noma lezi zinto zikhona ngamanani amancane ku-DNA ehlanzekile, zizoba nomthelela ekuphenduleni komfula, njenge-PCR kanye nokuvinjelwa kokugaya kwe-enzyme.Ngakho-ke, isihluthulelo sokuhlanza i-DNA yenhlabathi yindlela yokukhipha ngempumelelo ama-inhibitors emhlabathini.
Le khithi isebenzisa ikholomu ye-DNA-Only engabophezela ku-DNA, i-Foregene Protease entsha sha kanye nesistimu ye-buffer eyingqayizivele, engasusa ngempumelelo ama-inhibitor ahlukahlukene emhlabathini ngaphandle kokukhipha i-organic solvent noma i-ethanol ne-isopropanol.Ukukhishwa kwe-DNA kumasampula enhlabathi kungaqedwa ngemizuzu engama-90.
Imininingwane
25 Preps, 50 Preps, 100 Preps, 250 Preps
Izingxenye zekhithi
| I-Buffer SL1 |
| I-Buffer SL2 |
| I-Buffer SL3 |
| Isilondolozi se-PW |
| Isilondolozi se-WB |
| Isilondolozi EB |
| Isilondolozi TE |
| I-Foregene Protease |
| I-Lysozyme |
| Ikholomu Ye-DNA Kuphela |
| Iziyalezo |
Izici&izinzuzo
-Akukho ukungcola kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
-Isivinini esisheshayo: Ukusebenza kulula, futhi ukusebenza kwe-fecal genomic DNA extraction kungaqedwa kungakapheli imizuzu engama-40.
-Kulula: I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
-Ukuphepha: Akukho kukhishwa kwe-organic reagent edingekayo.
-Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, engahlangabezana nezidingo zokuhlola okuhlukahlukene.
Isicelo sekhithi
Ilungele ukuhlanzwa kwe-DNA ye-genomic kusuka kumasampuli alandelayo: umuntu omusha noma oqandisiwe, inkomo, inkukhu, igundane namanye amasampula endle.
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
-Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.
Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.
-Isixazululo se-Forgene Protease sinefomula eyingqayizivele, esebenzayo uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezingu-3), umsebenzi wayo nokuzinza kuyoba ngcono uma igcinwe ku-4 ° C, ngakho-ke kunconywa ukuyigcina ku-4 ° C, khumbula ukuthi ungayigcini ku -20 ° C.
-Dry Lysozyme powder igcinwa ku -20 ° C, isisombululo se-Lysozyme esilungisiwe sihlukaniswe izingxenye ezincane futhi sigcinwe ku -20 ° C.
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