I-DAPI Counterstain
Incazelo yekhithi:
Ingxenye eyinhloko yesisombululo se-DAPI sokubuyisela kabusha i-4-phenylindole, engangena ku-membrane yeseli futhi ibophe ngokuqinile ku-DNA.
Incazelo
Ingxenye eyinhloko yesisombululo se-DAPI sokubuyisela kabusha i-4-phenylindole, engangena ku-membrane yeseli futhi ibophe ngokuqinile ku-DNA.I-nucleus ye-fluorescent eluhlaza okwesibhakabhaka ingabonakala ngaphansi kwenjabulo yokukhanya kwe-ultraviolet ngaphansi kwesibonakhulu se-fluorescence.
Izingxenye zekhithi
| Isakhi | Imininingwane | ||
| 5Izivivinyo | 10Izivivinyo | 20Izivivinyo | |
| I-DAPICounterstain | 50μl | 200μl | 500μl |
Isicelo sekhithi
Isetshenziselwa ukungcolisa ama-nuclei.
Izidingo zesifanekiso
Uhlobo lwesifanekiso esisebenzayo: izicubu noma amasampula eseli atholwe yi-in situ hybridization.
Ukulinganiselwa
Le ndlela isebenza kuphela ekungcolisweni kwamasampula ezicubu noma amasampula eseli atholwe yi-in situ hybridization, futhi ayisebenzi ekungcolisweni kwamasampuli atholwe ngezinye izindlela.
Isitoreji kanye Nempilo yeshelufu
Igcinwe ku -20℃±5℃ ebumnyameni, isebenza izinyanga eziyi-12.Ezokuthutha ngaphansi kuka-8 ℃.
