I-RT Easyᵀᴹ II Master Premix ye-cDNA yomucu wokuqala we-Real Time PCR
Incazelo yekhithi:
- Uhlelo olusebenzayo lokuhlehla okulotshiweyo, kuthatha imizuzu engu-20 kuphela ukuqedela ukuhlanganiswa kwe-cDNA yomucu wokuqala.
- High-sensitivity reverse transcription system, izifanekiso zezinga le-pg nazo zingathola i-cDNA yekhwalithi ephezulu.
- Isistimu yokubhala ngokuhlehlayo inokuqina okuphezulu kwe-thermal, izinga lokushisa lokusabela lesistimu lingaba phezulu ngokufika ku-50 ℃, futhi linombhalo omuhle wokuhlehla wokusebenza.
- Ngisho namasampula e-RNA angcolile (utshwala obufika ku-60%, usawoti we-guanidine ofika ku-750mM) angaba ngaphansi kokusabela okuhlanekezelwe okulotshiweyo.
- 2× RT NOMA-KululaTM I-Mix yengeze ama-primer okulotshiweyo athuthukisiwe (Random Primer, Oligo(dT)18 Isiqalo).
Izincazelo zekhithi
I-Foregene's 2× RT NOMA-EasyTMImiksi ingahlanganisa i-cDNA yomucu wokuqala ezingeni lokushisa elifinyelela ku-50°C, elihambisana nokusabela kokuhlehla kokulotshwa kwezifanekiso zesakhiwo sesibili se-RNA.
I-RNA ehlanjululwe elabhorethri ngokuvamile iqukethe izinsalela zotshwala kanye ne-guanidine, ezinezici eziqinile zokuvinjelwa kwama-transcriptases amaningi ahlanekezela, okuholela emiphumeleni yokubhala ehlehlayo engagculisi noma ukusebenza kahle okuphansi kokuhlehla kokuloba.I-Foregene 2× RT Mix ikhombisa ukubekezelela okuphezulu kakhulu utshwala nosawoti we-guanidine.Ukubekezelela okuphezulu kakhulu utshwala kumasampula e-RNA ngu-60%, futhi ukubekezelela okuphezulu kakhulu kusawoti we-guanidine ngu-750mM.Ngisho ne-RNA engahlanzekile ingasetshenziselwa ukuhlehla kokubhala okubhaliwe nge-Foregene 2× RT NOMA-EasyTMHlanganisa.
2× RT NOMA-KululaTMI-Mix iyi-reagent esheshayo yokuhlehla yokubhala ethuthukiswe ngokukhethekile i-Real Time PCR.Le sistimu inekhono eliphezulu lokuloba okuhlanekezelwe futhi ayidingi ukwengeza noma yiziphi iziqalo, futhi ingenza ukusabela okuhle kokuhlehla kokulotshiweyo enanini elincane lezifanekiso ze-RNA.Isistimu yokusabela ehlukile yenza ukusabela kwe-RT kube lula, kusheshe futhi kusebenze kahle.I-strand cDNA synthesis yokuqala ingaqedwa ngemizuzu engama-20.Lo mkhiqizo ungasetshenziswa ngokuhambisana nomkhiqizo we-Foregene's fluorescent quantitative Real Time PCR EasyTMukuthola imiphumela yokuhlola yekhwalithi ephezulu.
Imininingwane
| I-RT EasyTMII I-Master Premix ye-first-strand cDNA synthesis ye-Real Time PCR | |||
| Izingxenye zekhithi | I-RT-01021 | I-RT-01022 | RT-01023 |
| 50 T (10μl uhlelo) | 200 T(10μl uhlelo) | 800 T (10μl uhlelo) | |
| 2× RT NOMA-KululaTMHlanganisa | 0.25ml | 1ml | 1ml 4 |
| I-ddH yamahhala ye-RNase2O | 1.7ml | 1.7ml | 1.7ml × 3 |
| Incwadi Yeziqondiso | 1 isiqephu | 1 isiqephu | 1 isiqephu |
Izici&izinzuzo
- Uhlelo olusebenzayo lokuhlehla okulotshiweyo, kuthatha imizuzu engu-20 kuphela ukuqedela ukuhlanganiswa kwe-cDNA yomucu wokuqala.
- High-sensitivity reverse transcription system, izifanekiso zezinga le-pg nazo zingathola i-cDNA yekhwalithi ephezulu.
- Isistimu yokubhala ngokuhlehlayo inokuqina okuphezulu kwe-thermal, izinga lokushisa lokusabela lesistimu lingaba phezulu ngokufika ku-50 ℃, futhi linombhalo omuhle wokuhlehla wokusebenza.
- Ngisho namasampula e-RNA angcolile (utshwala obufika ku-60%, usawoti we-guanidine ofika ku-750mM) angaba ngaphansi kokusabela okuhlanekezelwe okulotshiweyo.
- 2× RT NOMA-KululaTMI-Mix yengeze ama-primer okulotshiweyo athuthukisiwe (Random Primer, Oligo(dT)18Isiqalo).
Isicelo sekhithi
- Isetshenziswa ngokuqondile ku-Real Time PCR ukuze kuhlaziywe ngokwezibalo ukusho kofuzo
- Ingakwazi ukuhlaziya ngokushesha nangokunembile amanani amancane e-RNA njengamagciwane e-RNA
- Ukubhalwa kabusha okulotshiweyo kwezifanekiso ze-RNA ezinokuqukethwe okuphezulu kwe-GC noma ukwakheka kwesibili okuyinkimbinkimbi
Ukugeleza komsebenzi
Isitoreji nempilo yeshelufu
1. Izimo zokuhamba
Yonke inqubo yokuthutha kwebhokisi leqhwa elinezinga lokushisa eliphansi, ukuqinisekisa ukuthi ikhithi isesimweni esingu-<4°C.
2. Izimo zokugcina
I-RT EasyTMII igcinwa ku -20°C.Gcina umkhiqizo esiqandisini esingaguquki sezinga lokushisa elingu-20°C ngokushesha ngemva kokuwuthola.Uma izimo zesitoreji zifanelekile, umkhiqizo ngeke wehlise noma yikuphi ukusebenza phakathi nesikhathi sokuqinisekisa sonyaka ongu-1.
