I-Viral RNA Isolation Kit Yokuhlanzwa kwe-Viral RNA ku-Plasma, Serum, Nakwamanye Amasampuli
Incazelo yekhithi:
I-Cat.No.RE-02011/02014
Ukuze kuhlanzwe i-RNA yegciwane ku-plasma, i-serum, uketshezi lomzimba olungenamaseli, ama-cell-culture supernatants.
Khipha futhi uhlanze i-RNA yegciwane ngokushesha kumasampula afana ne-plasma, i-serum, uketshezi lomzimba olunganamaseli kanye nezinto ezingaphezu kwamandla amaseli.
-Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.Yonke ikhithi i-RNase-Free
-Kulula—yonke imisebenzi iqedwa ezingeni lokushisa legumbi
-Fast-ukusebenza kungaqedwa ngemizuzu engama-20
-Isivuno esiphezulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo
-Kuphephile—akukho mshini wokwenza izinto eziphilayo osetshenzisiwe
-Umthamo omkhulu wokucubungula isampula - amasampula angafika ku-200μl angacutshungulwa isikhathi ngasinye.
-Ikhwalithi ephezulu—i-RNA ecwengiwe imsulwa kakhulu, ayinaprotheyini nokunye ukungcola, futhi ingahlangabezana nezinhlelo zokusebenza zokuhlola ezehla nomfula.
Izincazelo
Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA yegciwane lesandulela ngculaza esezingeni eliphezulu kumasampula afana ne-plasma, i-serum, uketshezi lomzimba olunganaseli, kanye ne-cell culture supernatant.Ikhithi yengeza ngokuqondile i-Linear Acrylamide, engathwebula kalula amanani amancane e-RNA kumasampuli.Ikholomu Ye-RNA Kuphela ingabopha i-RNA ngokuphumelelayo.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.
Yonke ikhithi ayinayo i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe.I-Buffer viRW1 kanye ne-Buffer viRW2 ingaqinisekisa ukuthi i-viral nucleic acid etholwe ayinawo amaprotheni, i-nuclease noma okunye ukungcola, engasetshenziswa ngokuqondile ekuhloleni kwebhayoloji yamangqamuzana angezansi.
Imininingwane
50 Preps, 200 Preps
Izingxenye zekhithi
| Umugqa we-Acrylamide |
| I-Buffer viRL |
| I-Buffer viRW1, Buffer viRW2 |
| I-ddH yamahhala ye-RNase2O |
| Ikholomu Ye-RNA Kuphela |
| Iziyalezo |
Izici&izinzuzo
-Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.Yonke ikhithi i-RNase-Free
-Kulula—yonke imisebenzi iqedwa ezingeni lokushisa legumbi
-Fast-ukusebenza kungaqedwa ngemizuzu engama-20
-Isivuno esiphezulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo
-Kuphephile—akukho mshini wokwenza izinto eziphilayo osetshenzisiwe
-Umthamo omkhulu wokucubungula isampula - amasampula angafika ku-200μl angacutshungulwa isikhathi ngasinye.
-Ikhwalithi ephezulu—i-RNA ecwengiwe imsulwa kakhulu, ayinaprotheyini nokunye ukungcola, futhi ingahlangabezana nezinhlelo zokusebenza zokuhlola ezehla nomfula.
Imingcele yekhithi
Isicelo sekhithi:
Ilungele ukukhishwa nokuhlanzwa kwe-RNA yegciwane kumasampula afana ne-plasma, i-serum, uketshezi lomzimba olungenamaseli kanye ne-cell culture supernatant.
Ukugeleza komsebenzi
Izimo zokugcina
- Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25℃), noma 2-8℃ isikhathi eside.
Isixazululo se-Linear Acrylamide singagcinwa ekamelweni lokushisa izinsuku ezingu-7.Ngemva kokuthola ikhithi, sicela ukhiphe isisombululo se-Linear Acrylamide bese usigcina ku--20℃.
-Ngemva kokwengeza i-Linear Acrylamide ku-Buffer viRL, ingagcinwa ku-2-8℃ kuze kufike ku-48h.Sicela usebenzise isixazululo esisha esilungisiwe.
Imihlahlandlela yokuhlaziya izinkinga
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Ayikho i-RNA engakhishwa noma isivuno se-nucleic acid siphansi
Ngokuvamile kuba nezinto eziningi ezithinta ukusebenza kahle kokululama, njengalezi: isampula yokuqukethwe kwe-RNA, indlela yokusebenza, ivolumu ye-elution, njll..
Ukuhlaziywa kwezimbangela ezivamile:
1.Ibhavu yeqhwa noma izinga lokushisa eliphansi (4 ° C) i-centrifugation ngesikhathi sokusebenza.
Ukusikisela: Izinga lokushisa legumbi (15-25 ° C), ungalokothi ugeze eqhweni kanye nezinga lokushisa eliphansi le-centrifuge.
2. Ukugcinwa kwesampula okungalungile noma isampula yokugcina isikhathi eside kakhulu.
Ukusikisela: Gcina amasampula ku- -80 ° C noma ufrize ku-nitrogen ewuketshezi, futhi ugweme ukusetshenziswa kokuqhwaza okuphindaphindiwe;zama ukusebenzisa amasampula asanda kuqoqwa ukuze kukhishwe i-RNA.
3.Isampula yesampula enganele
Okutuswayo: Sicela uqinisekise ukuthi isampula kanye nesixazululo sokusebenza (i-Linear Acrylamide) kuxutshwe kahle futhi kufakwe ekufukameleni imizuzu eyi-10 ekamelweni lokushisa (15-25 ° C)
4.I-eluent yengezwe ngokungalungile
Okutuswayo: Qiniseka ukuthi i-ddH2O ye-RNase-Free yengezwa phakathi nolwelwesi lwekholomu yokuhlanza.
5.Ivolumu engafanele ye-ethanol engenamanzi ku-Buffer viRW2
Ukusikisela: Sicela ulandele imiyalelo, wengeze umthamo olungile we-ethanol enganamanzi ku-Buffer viRW2 bese uyixuba kahle ngaphambi kokusebenzisa ikhithi.
6.Ukusetshenziswa kwesampula okungalungile.
Isiphakamiso: 200µl wesampula ngo-500μl ngamunye we-Buffer viRL.Ivolumu yesampula eyeqile izoholela ekwehliseni izinga lokukhishwa kwe-RNA.
7.Ivolumu ye-elution engafanele noma ukungezwani okuphelele.
Isiphakamiso: Umthamo ongacacile wekholomu yokuhlanza ngu-30-50μl;uma umphumela we-elution ungagculisi, kuyanconywa ukuthi wengeze i-ddH eshisiwe ngaphambilini ye-RNase-Free2O futhi wandise isikhathi obeka ekamelweni lokushisa, ezifana 5-10min
8.Ikholomu yokuhlanza inezinsalela ze-ethanol ngemva kokuyihlanza ku-Buffer viRW2.
Isiphakamiso: Uma i-ethanol isasele ngemva kokugeza ku-Buffer viRW2 kanye ne-empty-tube centrifugation imizuzu engu-2, ikholomu yokuhlanza ingashiywa ekamelweni lokushisa imizuzu emi-5 ngemva kokuhlanganisa ishubhu engenalutho ukuze ikhiphe ngokuphelele i-ethanol esele.
Ukuwohloka kwama-molecule e-RNA ahlanzekile
Ikhwalithi ye-RNA ehlanziwe ihlobene nezici ezifana nokugcinwa kwesampula, ukungcoliswa kwe-RNase, nokusebenza.
Ukuhlaziywa kwezimbangela ezivamile:
1.Amasampuli aqoqiwe awalondolozwanga ngesikhathi.
Isiphakamiso: Uma isampula lingasetshenziswa ngesikhathi ngemuva kokuqoqwa, sicela uyigcine ku- -80 ℃ noma i-nitrogen ewuketshezi ngokushesha.Ukuze ukhiphe ama-molecule e-RNA, zama ukusebenzisa amasampula asanda kuqoqwa noma nini lapho kungenzeka.
2.Amasampula aqoqiwe abeqhwa futhi ancibilika ngokuphindaphindiwe.
Isiphakamiso: Gwema ukuqhwaza okuphindaphindiwe nokuncibilika (okungaphezu kokukodwa) phakathi nokuqoqwa nokugcinwa kwesampula, ngaphandle kwalokho isivuno se-nucleic acid sizokwehla.
3.I-RNase yethulwa egunjini lokuhlinzela noma kwakungekho amagilavu alahlwayo, imaski, njll.
Isiphakamiso: Ukukhishwa kokuhlolwa kwama-molecule e-RNA kwenziwa kangcono ekamelweni lokusebenza elihlukile le-RNA, futhi ithebula lokuhlola liyahlanzwa ngaphambi kokuhlolwa.Gqoka amagilavu alahlwayo kanye nemaski ngesikhathi sokuhlolwa ukuze ugweme ukucekelwa phansi kwe-RNA okubangelwa ukwethulwa kwe-RNase.
4.I-reagent ingcoliswe yi-RNase ngesikhathi isetshenziswa.
Isiphakamiso: Faka esikhundleni se-Viral RNA Isolation Kit entsha ukuze uthole ukuhlolwa okuhlobene.
5.Ukungcoliswa kwe-RNase kwamashubhu e-centrifuge, amathiphu e-pipette, njll. Isiphakamiso: Qiniseka ukuthi amashubhu e-centrifuge, amathiphu e-pipette, namapayipi konke Akuna-RNAse.
Ama-molecule e-RNA ahlanziwe athinte ukuhlolwa okungaphansi komfula
Ama-molecule e-RNA ahlanzwe ikholomu yokuhlanza azothinta ukuhlolwa okuya phansi komfula uma kukhona ama-ion kasawoti amaningi noma amaprotheni, afana nalokhu: ukuloba okuhlanekezelwe, i-Northern Blot, njll..
1.Kukhona ama-ion kasawoti asele kuma-athomu e-RNA e-luted.
Okutuswayo: Qiniseka ukuthi ivolumu elungile ye-ethanol enganamanzi yengezwe ku-Buffer viRW2, futhi ugeze ikholomu yokuhlanza kabili ngokwejubane elifanele le-centrifugation emiyalweni yokusebenza;Uma kusekhona ama-ion kasawoti asele, ungakwazi ukwengeza i-Buffer viRW2 kukholamu yokuhlanza, futhi uyishiye ekamelweni lokushisa imizuzu emi-5.Bese wenza i-centrifugation ukuze ususe ukungcoliswa kwama-ion kasawoti ngezinga elikhulu kakhulu
2.Kune-ethanol esele kuma-athomu e-RNA akhishwe
Isiphakamiso: uma usuqinisekisa ukuthi amakholomu okuhlanza ahlanjululwe yi-Buffer viRW2, yenza i- empty-tube centrifugation ngokwesivinini se-centrifugal emiyalweni yokusebenza.Uma isekhona i-ethanol esele, ingashiywa imizuzu emi-5 ekamelweni lokushisa ngemva kwe-centrifugation yeshubhu engenalutho ukuze kukhishwe i-ethanol esele ngezinga elikhulu kakhulu.
Amamanuwali wemiyalo:
I-Viral RNA Isolation Kit Manual
