I-RT Easyᵀᴹ I Master Premix yokuhlanganiswa kwe-cDNA yomucu wokuqala
Incazelo yekhithi:
- Uhlelo olusebenzayo lokuhlehla okulotshiweyo, kuthatha imizuzu engama-25 kuphela ukuqedela ukuhlanganiswa kwe-cDNA yomucu wokuqala.
- High-sensitivity reverse transcription system, izifanekiso zezinga le-pg nazo zingathola i-cDNA yekhwalithi ephezulu.
- Isistimu yokubhala okuphambene inokuqina okuphezulu kokushisa, izinga lokushisa lokusabela lesistimu lingafika ku-50 ℃,futhi inokusebenza okuhle kokuhlehla kokuloba.
- Ngisho namasampula e-RNA angcolile (utshwala obufika ku-45%, usawoti we-guanidine ofika ku-750mM) angaba ngaphansi kokusabela okuhlanekezelwe okulotshiweyo.
Izincazelo zekhithi
FOREGENE sika 2× RT EasyTMImiksi ingahlanganisa i-cDNA yomucu wokuqala ezingeni lokushisa elifinyelela ku-50°C, elihambisana nokusabela kokuhlehla kokulotshwa kwezifanekiso zesakhiwo sesibili se-RNA.
I-RNA ehlanjululwe elabhorethri ngokuvamile iqukethe izinsalela zotshwala kanye ne-guanidine, ezinezici eziqinile zokuvinjelwa kwama-transcriptases amaningi ahlanekezela, okuholela emiphumeleni yokubhala ehlehlayo engagculisi noma ukusebenza kahle okuphansi kokuhlehla kokuloba.FOREGENE 2× RT EasyTMImiksi ibonisa ukubekezelela okuphakeme kakhulu kotshwala nosawoti we-guanidine, ukubekezelelwa okuphezulu kakhulu kotshwala kumasampula e-RNA ngu-45%, futhi ukubekezelela okuphezulu kakhulu kusawoti we-guanidine ngu-750mM.Ngisho noma i-RNA imsulwa, ukusabela okulotshiwe okuphambene kungenziwa nge-FOREGENE 2×RT Mix.Isistimu yokusabela eyingqayizivele yenza ukusabela kwe-RT kube lula, kusheshe futhi kusebenze kahle, futhi ukuhlanganiswa kwe-cDNA yomucu wokuqala kungaqedwa emizuzwini engama-25.
Lo mkhiqizo ungasetshenziswa ngokuhambisana nomkhiqizo we-Foregene's fluorescent quantitative Real Time PCR EasyTMukuthola imiphumela yokuhlola yekhwalithi ephezulu.
Imininingwane
| I-RT EasyTMI I-Master Premix ye-first-strand cDNA synthesis | ||
| Okuqukethwe kwekhithi | I-RT-01011 | I-RT-01012 |
| Izikhathi ezingama-25 (uhlelo lwe-20μl) | Izikhathi eziyi-100 (20μl system) | |
| 2× RT EasyTM Mix | 0.25ml | 1ml |
| I-Primer engahleliwe (50μM) | 50μl | 200μl |
| I-Oligo(dT)18 Primer (50μM) | 50μl | 200μl |
| RNase-Free ddH2O | 1.7ml | 1.7ml |
| Imanuwali Yeziqondiso | 1 isiqephu | 1 isiqephu |
Izici&izinzuzo
- Uhlelo olusebenzayo lokuhlehla okulotshiweyo, kuthatha imizuzu engama-25 kuphela ukuqedela ukuhlanganiswa kwe-cDNA yomucu wokuqala.
- High-sensitivity reverse transcription system, izifanekiso zezinga le-pg nazo zingathola i-cDNA yekhwalithi ephezulu.
- Isistimu yokubhala ngokuhlehlayo inokuqina okuphezulu kwe-thermal, izinga lokushisa lokusabela lesistimu lingaba phezulu ngokufika ku-50 ℃, futhi linombhalo omuhle wokuhlehla wokusebenza.
- Ngisho namasampula e-RNA angcolile (utshwala obufika ku-45%, usawoti we-guanidine ofika ku-750mM) angaba ngaphansi kokusabela okuhlanekezelwe okulotshiweyo.
Isicelo sekhithi
- I-RT-PCR evamile
- Ukuhlanganiswa kwe-cDNA yocwaningo lwe-cloning kanye nenkulumo
- Ukuhlaziywa kwendlela yokwandiswa kwe-primer yesayithi yokuqalisa ukuloba
- RACE (ukukhulisa ngokushesha iziphetho ze-cDNA)
- Linear RNA amplification
- I-chip mark
Ukugeleza komsebenzi
Isitoreji nempilo yeshelufu
1.Izimo zokuhamba
Yonke inqubo yokuthutha kwebhokisi leqhwa elinezinga lokushisa eliphansi, ukuqinisekisa ukuthi ikhithi isesimweni esingu-<4 ℃.
2. Izimo zokugcina
I-RT EasyTMNgigcinwa ku -20°C.Gcina umkhiqizo esiqandisini esingaguquki sezinga lokushisa elingu-20°C ngokushesha ngemva kokuwuthola.Uma izimo zesitoreji zifanelekile, umkhiqizo ngeke wehlise noma yikuphi ukusebenza phakathi nesikhathi sokuqinisekisa sonyaka ongu-1.
