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I-Foregene DNA Identification System 38Y-C (Ikesi)

I-Foregene DNA Identification System 38Y-C (Ikesi) Isithombe Esifakiwe
  • I-Foregene DNA Identification System 38Y-C (Ikesi)
  • I-Foregene DNA Identification System 38Y-C (Ikesi)
  • I-Foregene DNA Identification System 38Y-C (Ikesi)
  • I-Foregene DNA Identification System 38Y-C (Ikesi)

Incazelo yekhithi:

I-Foregene Uhlelo lokuhlonza i-DNA 38Y-U-C usebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ye-fluorescent ukuze akhulise 38 Y chromosome STR loci , 3 Y-Indel locifuthiizindawo ezimbili zokulawula ikhwalithi yangaphakathi ngesikhathi esisodwaukuze kukhuliswe Amasampula ekesi akhishwe kuzifanekiso ze-DNA ezihlanzekile.

amandla angaphambili


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Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo

I-Foregene Uhlelo lokuhlonza i-DNA 38Y-U-C usebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ye-fluorescent ukuze akhulise 38 Y chromosome STR loci , 3 Y-Indel locifuthiizindawo ezimbili zokulawula ikhwalithi yangaphakathi ngesikhathi esisodwaukuze kukhuliswe Amasampula ekesi akhishwe kuzifanekiso ze-DNA ezihlanzekile.

Okuqukethwe kwekhithi

Ukulungiswa kwesistimu yokukhulisa

Iukulungiselela i-composition of Okujwayelekileukukhishwakanye nesistimu ye-amplification

 I-Master Mix

Cabaphikisayo

10μl uhlelo (μl)

2.5×PCR Master Mix C I

4.0

5 x 38Y-C I-Primer Mix

2.0

Isifanekiso se-DNA

1- 4

Amanzi enziwe deionized

Gcwalisa kufika kumthamo wokusabela we10 ml

Ukuhlaziywa Kwedatha

Igrafu enamathiselwe 1:Imephu ye-Allelic Ladder Typing

Igrafu enamathiselwe 2:Ukuthayipha kwe-DNA okujwayelekile okungu-9948imephu

I-Reagent Storage

1. Sicela uwagcine ngaphansi kokungu-20°C (uma engasetshenziswanga okwesikhashana), ngemva kokuthola amakhithi eqhwa eqhweni elomile noma emaphaketheni eqhwa ejeli.

2. Sicela usule ingxenyana eyinhloko ye-PCR yokusabela kwangaphambili iku--20°C, ngemva kokuba ikhithi ikhishiwe ukuze isetshenziswe futhi ama-reagents asele asabela ngaphambilini agcinwa ku-4°C ukugwema ukuqhwa okuphindaphindiwe nokuncibilika.Uma umthamo owodwa umncane, kunconywa ukuthi ugcinwe ku -20 ° C ngemuva kokukhipha i-aliquoting.

3. Gcina izingxenye ngemva kokusabela ku-4°C, gwema ukuqhwa okuphindaphindiwe nokuncibilika, futhi ungathinti ama-reagents ngaphambi kokusabela ukuze ugweme ukungcoliswa.

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