I-Blood DNA Midi Kit (1-5ml) I-Blood Isolation Midi Kits ye-DNA Egazini
Incazelo yekhithi:
Hlanza ngokushesha i-genomic DNA yekhwalithi ephezulu egazini elinqanyuliwe (1-5ml).
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
Incazelo
Isampula yegazi iqukethe inani elikhulu lamaseli abomvu egazi namaseli e-platelet, okwenza ukucubungula kwegazi kube nzima kakhulu.Kodwa-ke, i-DNA ye-genomic yegazi ekhishwe ngendlela evamile ayinakho ukuhlanzeka okuphezulu futhi okuqukethwe kuphansi kakhulu.Le kit isebenzisa i-Foregene Protease Plus entsha sha kanye ne-BL1 kanye ne-BL2 buffer systems ehlukile, ekwazi ukugayeka ngokuphelele igazi elinqandekayo futhii-coagulationamasampula ngesikhathi esifushane kakhulu, ngaleyo ndlela kugwenywe ukucekelwa phansi kwe-DNA ngezinga elikhulu kakhulu futhi kutholwe inani elikhulu kakhulu le-genomic DNA.Uhlelo olusheshayo lokucutshungulwa kwegazi kanye nokusebenza okulula kwekholomu ye-spin kwenza kube lula kakhulu ukukhishwa kwe-genome yegazi, ukuze i-DNA ye-genomic yekhwalithi ephezulu, emsulwa ingatholakala kungakapheli imizuzu engama-40.
Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu yokuphonswa kwaleli kit iyinto entsha eyingqayizivele yenkampani, ekhangisa kahle i-DNA, futhi ingasusa i-RNA, amaprotheni angcolile, ama-ion nezinye izinhlanganisela eziphilayo kumaseli ngezinga elikhulu kakhulu.Izingcezu ze-DNA ezitholiwe zinkulu, ezinobumsulwa obuphezulu, ikhwalithi ezinzile futhi enokwethenjelwa, futhi umthamo omkhulu wokuthwala wekholomu ye-spin yi-80 μg DNA.I-DNA etholiwe ingasetshenziselwa ukuhlolwa kwebhayoloji yamangqamuzana njengokuvinjelwa kokugaya, i-PCR, i-Southern hybridization, ukwakhiwa komtapo wolwazi nokunye.Ikhithi ingakwazi ukucubungula igazi elifika ku-5ml ngesikhathi ukuze lithole inani elanele le-DNA ye-genomic ephezulu.
Imininingwane
50 Preps, 100 Preps, 250 Preps
Izingxenye zekhithi
| Isilondolozi BL1 |
| Isilondolozi BL2* |
| I-Buffer DC |
| Isilondolozi PW* |
| Isilondolozi se-WB1 |
| Isilondolozi EB |
| I-Foregene Protease Plus |
| Ikholomu Ye-DNA Kuphela |
| Iziyalezo |
Izici&izinzuzo
-Akukho ukungcola kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukugwema ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
-Isivinini esisheshayo: I-Foregene Protease Plus inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi nemizuzu engama-40.
-Kulula: I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-4°I-C yezinga lokushisa eliphansi le-centrifugation noma imvula ye-ethanol ye-DNA.
-Ukuphepha: Akukho kukhishwa kwe-organic reagent edingekayo.
-Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, engahlangabezana nezidingo zokuhlola okuhlukahlukene.
Isicelo sekhithi
Ilungele ukukhishwa kanye nokuhlanzwa kwe-genomic DNA egazini elisha noma eliqandisiwe elilwa nokuqunjelwa.
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
-Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25℃);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8℃.
Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.
-Isixazululo se-Forgene Protease Plus sinefomula eyingqayizivele, esebenzayo uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezi-3), umsebenzi wayo nokuzinza kuzoba ngcono uma igcinwe ku-4 ° C, ngakho-ke kunconywa ukuyigcina ku-4 ° C, khumbula ukuthi ungayigcini ku -20℃.
