1kb DNA Ladder
Incazelo yekhithi:
◮I-1Kb DNA Ladder yakhiwe ngemicu ephindwe kabili engu-13 ye-DNA, futhi ukugxiliswa okuphindwe kabili kwe-1kb no-5kb kuyasiza ukuhlukanisa amabhendi ahlukene.Ngaphansi kuka-1kb, amabhendi amathathu we-250bp, 500bp, kanye no-750bp ayengeziwe, futhi usayizi wesiqephu esifushane bungabalwa cishe ngokusekelwe kulokhu.Ukugxila kwamabhendi amane we-6kb, 7kb, 8kb, kanye no-10kb kuhliswe ngohhafu, okuzuzisayo ukuthola umphumela wokuhlukanisa ongcono.
Izincazelo
I-1Kb DNA Ladder yakhiwe ngemicu ephindwe kabili engu-13 ye-DNA, futhi ukugxiliswa okuphindwe kabili kwe-1kb no-5kb kuyasiza ukuhlukanisa amabhendi ahlukene.Ngaphansi kuka-1kb, amabhendi amathathu we-250bp, 500bp, kanye no-750bp ayengeziwe, futhi usayizi wesiqephu esifushane bungabalwa cishe ngokusekelwe kulokhu.Ukugxila kwamabhendi amane we-6kb, 7kb, 8kb, kanye no-10kb kuhliswe ngohhafu, okuzuzisayo ukuthola umphumela wokuhlukanisa ongcono.
Lo mkhiqizo usuvele uqukethe i-1× Iyalayisha Ibhafa.Ungathatha i-2-6μl ye-electrophoresis eqondile ngokuya ngezidingo zokuhlolwa.Isithombe se-electrophoresis sicacile, sifanelekile futhi siyasebenza.
Amabhendi angu-13 alo mkhiqizo kusukela kwabancane kuye kwabakhulu yilawa: 250bp, 500bp, 750bp, 1kb, 1.5kb, 2kb, 3kb, 4kb, 5kb, 6kb, 7kb, 8kb, 10kb.Uma ivolumu yokulayisha ingu-5μl, amabhendi angu-1kb no-5kb angu-100ng;6kb, 7kb, 8kb, kanye namabhendi angu-10kb angu-25ng;futhi amabhande asele angama-50ng.
Imininingwane
| I-DL-30011 | 50 Preps (250μl) |
| I-DL-30012 | 200 Preps (1ml) |
Izingxenye zekhithi
Ulwazi loketshezi lwesitoreji
10mM Tris-HCl(pH 8.4) 10mM EDTA
0.02% BPB
5% glycerin
Ukugxila komkhiqizo
Ukugxila komkhiqizo cishe ku-130μg/ml
Electropherogram
Izimo zokugcina
Sicela ugcine ngo-4℃(sicela ugcine ku -20℃okokugcina isikhathi eside)
