I-China Genomic DNA I-Micro Kit I-Micro Genomic DNA Mini Kit Umkhiqizi Nomhlinzeki |I-Foregene

I-Genomic DNA Micro Kit Ikhithi encane yeGenomic DNA

Incazelo yekhithi:

Hlanza ngokushesha amasampula amancane ukuze uthole i-genomic DNA yekhwalithi ephezulu.

◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.

◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.

◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.

◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.

◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo

Le khithi isebenzisa Ikholomu Ye-DNA Kuphela engahlanganisa ngokuqondile i-DNA, i-Foregene Protease entsha sha kanye nesistimu ye-buffer eyingqayizivele, engakhipha ngempumelelo amasampula emvelo ahlukahlukene, njengamanani egazi, amaseli, nezilwane phakathi nemizuzu engama-20-80.Izicubu, amabala egazi omisiwe, ama-swabs kakotini asemtholampilo, izimpande zezinwele, njll., ziyahlanzwa ukuze kutholwe i-DNA ye-genomic ephezulu, yekhwalithi ephezulu.

Imininingwane

50 Preps

Izingxenye zekhithi

Isilondolozi se-ST1

Isigcinalwazi ST2*

Isilondolozi PW*

Isilondolozi se-WB

Isilondolozi EB

I-Foregene Protease

Ikholomu Ye-DNA Kuphela

Iziyalezo

Izici&izinzuzo

-Akukho ukungcola kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

-Isivinini esisheshayo: I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.

-Kulula: I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.

-Ukuphepha: Akukho kukhishwa kwe-organic reagent edingekayo.

-Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, engahlangabezana nezidingo zokuhlola okuhlukahlukene.

-Isistimu ye-Micro-elution: Ingakhuphula ukuhlangana kwe-DNA ye-genomic, elungele ukutholwa noma ukuhlolwa komfula.

Isicelo sekhithi

Ifanele ukuhlanzwa kwamasampula alandelayo e-DNA ye-genomic: izicubu zezilwane ezintsha noma eziqandisiwe, amanani amasha noma anqande ukucoagulated cryopreserved egazi lonke, amachashaza egazi omisiwe, amaseli akhulisiwe, izicubu ze-microsection, izimpande zezinwele, okokugeza umlomo, njll.

Ukuhamba komsebenzi

Isitoreji kanye Nempilo yeshelufu

-Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.

Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.

-Isixazululo se-Forgene Protease sinefomula eyingqayizivele, esebenzayo uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezingu-3);umsebenzi wayo nokuzinza kuzoba ngcono uma igcinwe ku-4°C, ngakho-ke kunconywa ukuthi ugcine ku4°C, khumbula ukuthi ungayigcini ku-20°C.

Okwedlule: I-Buccal Swab/FTA Card DNA Isolation Kit Genomic DNA Extraction noma Ikhithi Yokuhlanzwa ku-Buccal Swabs

Olandelayo: 10ul Pipette Amathiphu

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