Umkhiqizi Nomhlinzeki Wekhithi Yokuzihlukanisa Ye-DNA Yase-China |I-Foregene

Ikhithi yokuhlukanisa i-DNA yamahhala

Ikhithi Yokuzihlukanisa Ye-DNA Yamahhala Efakiwe Isithombe

Incazelo yekhithi:

Ukuhlukaniswa kwe-cfDNA kumasampula e-plasma

Inombolo yekhathalogi TQ01BT0050, TQ01BT0100

Thumela i-imeyili kithi Landa njenge-PDF

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Incazelo

I-DNA engenamangqamuzana (cfDNA) iwucezu lwe-cfDNA olukhishwe kumaseli luye emgudwini wegazi olungathwala ulwazi lwebhayoloji lusuka.izimila, amagciwane kanye ne-fetus.Ukuhlaziywa kwe-cfDNA kunikeza izimpawu ezibalulekile zokuxilongwa, ukutholwa kanye nokuqapha ukuqhubeka kwesifoumbono.Njengamanje, lobu buchwepheshe busetshenziswe kabanzi emikhakheni yokutholwa kokuguqulwa kwesimila, ukuqondiswa kwezidakamizwa okuhlosiwe kanye nokubikezela,ukutholwa kwe-pathogen kanye nokuhlolwa kokubeletha okungahlaseli.

Ikhwalithi ephezulu ye-nucleic acid iyisiqinisekiso socwaningo lwe-genomics.I-Foregene isebenzisa ubuchwepheshe bokuhlanza ubuhlalu be-superparamagneticukuhlinzeka abasebenzisi ngesisombululo esibucayi kakhulu sokukhipha i-nucleic acid esifinyelela kalula ukukhishwa kwe-nucleic acid ephansiukugxilisa ingqondo.Njengoba kuqinisekiswa izinkulungwane zamasampula omtholampilo, lo mkhiqizo ungakhipha i-cfDNA yekhwalithi ephezulu oketshezini lomzimba olungenamaselinjenge-plasma entsha noma eqandisiwe, i-serum, uketshezi lwe-pleural, ama-ascites noketshezi lwe-cerebrospinal ocwaningweni lwesayensi kanye nokuxilongwa kwe-in-vitro yomtholampilosebenzisa.

I-Foregene Cell-free DNA Isolation Kit yakhelwe ukuhlukanisa i-DNA ejikelezayo yamaseli (cfDNA) kuplasma yomuntu, i-serum, uketshezi lomzimba, noma amasampula omchamo.Ikhithi ingakwazi ukuhlukanisa ngokushesha futhi kalula i-cfDNA ngezinyathelo ezintathu: i-lysis/ ukubopha, ukuwashwa kanye nokwelula.Isebenzisa ubuhlalu be-superparamagnetic obuklanywe ngokukhethekile nge-silica-coated superparamagnetic enohlelo oluhlukile lwe-lysis/ lokubopha ukuze kubuyiselwe ama-nucleic acid ajikelezayo nge-hydrogen bonding namandla kagesi, ngaphandle kokukhangisa amaprotheni nokunye ukungcola.Ilungele iziteshi zokusebenza zokukhipha okuzenzakalelayo ezisebenza ngokuzenzakalelayo ukulungiselela noma yikuphi ukuhlaziywa komfula kusetshenziswa i-PCR noma i-NGS.

Okuqukethwe kwekhithi kanye Nesitoreji

Ithebula 1 Foregene Cell-free DNA Isolation Kit

Cizinto	Rozimisele	Inani(25 T)	Inani(50 T)	Inani(100 T)	Sukubekezela
Ibhokisi1	Isivimbeli AL	42 mLx1 ibhodlela	89 mLx1 ibhodlela	189 mLx1 ibhodlela	1 0- 30°C
	Isilondolozi se-AW1	38 mLx1 ibhodlela	83 mLx1 ibhodlela	165 mLx1 ibhodlela	1 0- 30°C
	Isilondolozi se-AW2	15 mLx1 ibhodlela	33 mLx1 ibhodlela	66 mLx1 ibhodlela	1 0- 30°C
	I-Elution	3 mLx1 ibhodlela	6 mLx1 ibhodlela	12 mLx1 ibhodlela	1 0- 30°C
Ibhokisi2	Ubuhlalu obuzibuthe A	3 .2 mLx1 ibhodlela	6 .5 mLx1 ibhodlela	13 mLx1 ibhodlela	2 - 8 ° C
Ibhokisi2	Amaprotheni K	5 mLx1 ibhodlela	10 .5 mLx1 ibhodlela	21 mLx1 ibhodlela	2 - 8 ° C

Amanothi:

[1] Ungaxubi izingxenye zamaqoqo ahlukene ekhithi.

[2] Ibhafa AL ingase yakhe imvula, engathinti umsebenzi wayo.Uma imvula ibonakala, sicela ubeke ibhodlela le-reagent kubhavu wamanzi ongu-56 °C imizuzu engu-1 0 - 20 kuze kuncibilike imvula.Bese uxuba kahle ngaphambi kokusetshenziswa.

[3] Ungabuvimbi ubuhlalu obuzibuthe A.

Inqubo

Izici&izinzuzo

-Isizinda Esisebenzayo Isivuno esiphezulu sokukhishwa kwe-DNA, ukuhlanzeka okuhle, ukususwa okusebenzayo kokungcola kanye nama-PCR inhibitors angezansi;

I-Flexible Sample IncorporationUkukhishwa okulula kwe-cfDNA kusuka ku-0.5mL kuya ku-4mL amasampula anevolumu ephansi njenge-20uL

Izicelo EzihlukahlukeneKuqinisekiswe amasampula omtholampilo, aklanyelwe isimila, ukutheleleka kanye nokuxilongwa ngaphambi kokubeletha nge-liquid biopsy

Ukusekelwa okubanzi kwe-Automated Platform Ihambisana nezinqubo zokukhipha ezizenzakalelayo zemanuwali, isekela i-Tecan, i-Hamilton, i-PE nezinye izindawo zokusebenza zokukhipha i-nucleic acid.

Okwedlule: Isampula Yekhwalithi Enhle Yokukhishwa I-Reagent Nucleic Acid Extraction-Free Vtm Kit Isinyathelo esisodwa Indlela Yokuhlola ye-PCR

Olandelayo: Ikhithi Yesitshalo Se-Dnasecure Yentengo Ephansi Kakhulu Ye-DNA Extraction Nucleic Acid Purification

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