I-PCR Easyᵀᴹ (enedayi)
Incazelo yekhithi:
Iqhawe le-2 × PCRTM Isistimu ye-Mix inokubekezelela okuphezulu kuma-PCR inhibitors kunesistimu evamile ye-PCR Mix, futhi ingabhekana kalula nokukhulisa i-PCR yezifanekiso eziyinkimbinkimbi ezihlukahlukene.Isistimu yokusabela eyingqayizivele kanye nokusebenza kahle okuphezulu kwe-Taq Hero kwenza ukusabela kwe-PCR kube nokusebenza kahle kokukhulisa ukuphakama, ukucacisa nokuzwela.
Izincazelo
Lo mkhiqizo uhlanganisaI-ForegeneIsizukulwane esisha esiyingqayizivele sika-Taq——Forregene Taq Hero, optimized dNTPs, MgCl2, isilondolozi sokusabela, isithuthukisi sokusabela kwe-PCR, isikhuthazi kanye nesiqinisi.Iqhawe le-2 × PCRTM Isistimu ye-Mix inokubekezelela okuphezulu kuma-PCR inhibitors kunesistimu evamile ye-PCR Mix, futhi ingabhekana kalula nokukhulisa i-PCR yezifanekiso eziyinkimbinkimbi ezihlukahlukene.Isistimu yokusabela eyingqayizivele kanye nokusebenza kahle okuphezulu kwe-Taq Hero kwenza ukusabela kwe-PCR kube nokusebenza kahle kokukhulisa ukuphakama, ukucacisa nokuzwela.
Umkhiqizo uxutshwe ngaphambili ne-Electrophoresis Loading Buffer, futhi ngemva kokuba i-PCR isiqediwe, isampula ingalayishwa ngokuqondile nge-electrophoresis;ukusebenza kulula futhi Kalula nakakhulu.
Imininingwane
Ikhithi Series
DP-20032-B:2× PCR EasyTMIngxube(udayi) 5ml(1.7ml × 3)
DP-20033-B:2× PCR EasyTMIngxube(udayi) 10ml(1.7ml × 6)
DP-20033-B:2× PCR EasyTMIngxube(udayi) 40ml(1.7ml × 24)
Izingxenye zekhithi
2× PCR KululaTMImiksi(udayi)hlanganisa:0.1U Foregene Taq DNA Polymerase/μl,400 μM dNTPs,20 mM Tris-HCl(pH8.8),100 mM KCl, 3 mM MgCl2, amanye ama-stabilizers kanye nezithuthukisi,I-DNA Loading Buffer.
Izici&izinzuzo
Iqhawe le-2 × PCRTM Isistimu ye-Mix inokubekezelela okuphezulu kuma-PCR inhibitors kunesistimu evamile ye-PCR Mix, futhi ingabhekana kalula nokukhulisa i-PCR yezifanekiso eziyinkimbinkimbi ezihlukahlukene.Isistimu yokusabela eyingqayizivele kanye nokusebenza kahle okuphezulu kwe-Taq Hero kwenza ukusabela kwe-PCR kube nokusebenza kahle kokukhulisa ukuphakama, ukucacisa nokuzwela.
Imingcele yekhithi
Isicelo sekhithi
-I-PCR yokukhulisa izingcezu ze-DNA
- Ukulebula kwe-DNA
- Ukulandelana kwe-DNA
-PCR plus A umsila
Izimo zokugcina
Ingagcinwa isikhathi eside ku -20°C;ukuze isetshenziswe njalo, ingagcinwa ku-4°C isikhathi esifushane.
